Restriction map for
pFN21AE4420
(Linear) (Six-base) MAPSORT of: KIEE4420.seq Check: 8341 from: 1 to: 1305
KIEE4420.orf
With 182 enzymes: SgfI *
March 22, 2012 17:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 20 1305
Size: 20 1285
AfeI AGC'GCT
Cuts at: 0 448 1305
Size: 448 857
AleI CACnn'nnGTG
Cuts at: 0 1281 1305
Size: 1281 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 757 1305
Size: 757 548
ApaI G_GGCC'C
Cuts at: 0 73 1037 1065 1190 1305
Size: 73 964 28 125 115
Fragments arranged by size:
964 125 115 73 28
BanI G'GyrC_C
Cuts at: 0 125 238 398 553 1204 1275 1305
Size: 125 113 160 155 651 71 30
Fragments arranged by size:
651 160 155 125 113 71 30
BanII G_rGCy'C
Cuts at: 0 73 1037 1065 1190 1305
Size: 73 964 28 125 115
Fragments arranged by size:
964 125 115 73 28
BbeI G_GCGC'C
Cuts at: 0 402 1305
Size: 402 903
BbsI GAAGACnn'nnnn_
Cuts at: 0 156 1305
Size: 156 1149
BglI GCCn_nnn'nGGC
Cuts at: 0 316 547 596 668 940 1305
Size: 316 231 49 72 272 365
Fragments arranged by size:
365 316 272 231 72 49
BglII A'GATC_T
Cuts at: 0 863 1305
Size: 863 442
Bme1580I G_kGCm'C
Cuts at: 0 73 241 459 576 664 681 723 763
Size: 73 168 218 117 88 17 42 40
Cuts at: 763 1037 1065 1190 1209 1305
Size: 274 28 125 19 96
Fragments arranged by size:
274 218 168 125 117 96 88 73
42 40 28 19 17
BmgBI CAC'GTC
Cuts at: 0 150 1305
Size: 150 1155
BmrI ACTGGGnnnn_n'
Cuts at: 0 114 272 396 429 1039 1305
Size: 114 158 124 33 610 266
Fragments arranged by size:
610 266 158 124 114 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 973 1305
Size: 973 332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 544 775 928 968 1305
Size: 151 393 231 153 40 337
Fragments arranged by size:
393 337 231 153 151 40
Bpu10I CC'TnA_GC
Cuts at: 0 1192 1305
Size: 1192 113
BsaHI Gr'CG_yC
Cuts at: 0 399 1305
Size: 399 906
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 1305
Size: 259 30 1016
Fragments arranged by size:
1016 259 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 482 1126 1129 1305
Size: 482 644 3 176
Fragments arranged by size:
644 482 176 3
BseYI C'CCAG_C
Cuts at: 0 230 234 407 564 730 968 1305
Size: 230 4 173 157 166 238 337
Fragments arranged by size:
337 238 230 173 166 157 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 1305
Size: 518 787
BsiHKAI G_wGCw'C
Cuts at: 0 914 959 1090 1305
Size: 914 45 131 215
Fragments arranged by size:
914 215 131 45
BsmI GAATG_Cn'
Cuts at: 0 114 300 805 1305
Size: 114 186 505 500
Fragments arranged by size:
505 500 186 114
Bsp1286I G_dGCh'C
Cuts at: 0 73 241 459 576 664 681 723 763
Size: 73 168 218 117 88 17 42 40
Cuts at: 763 914 959 1037 1065 1090 1190 1209 1305
Size: 151 45 78 28 25 100 19 96
Fragments arranged by size:
218 168 151 117 100 96 88 78
73 45 42 40 28 25 19 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 20 360 1305
Size: 20 340 945
Fragments arranged by size:
945 340 20
BsrBI CCG'CTC
Cuts at: 0 1290 1305
Size: 1290 15
BsrFI r'CCGG_y
Cuts at: 0 86 440 652 843 1305
Size: 86 354 212 191 462
Fragments arranged by size:
462 354 212 191 86
BstEII G'GTnAC_C
Cuts at: 0 829 1305
Size: 829 476
BstYI r'GATC_y
Cuts at: 0 863 1305
Size: 863 442
Bsu36I CC'TnA_GG
Cuts at: 0 494 1305
Size: 494 811
BtgI C'CryG_G
Cuts at: 0 198 1095 1279 1305
Size: 198 897 184 26
Fragments arranged by size:
897 198 184 26
BtsI GCAGTG_nn'
Cuts at: 0 574 755 1305
Size: 574 181 550
Fragments arranged by size:
574 550 181
DraIII CAC_nnn'GTG
Cuts at: 0 122 144 795 1305
Size: 122 22 651 510
Fragments arranged by size:
651 510 122 22
DrdI GACnn_nn'nnGTC
Cuts at: 0 154 1305
Size: 154 1151
EaeI y'GGCC_r
Cuts at: 0 484 654 797 1092 1098 1305
Size: 484 170 143 295 6 207
Fragments arranged by size:
484 295 207 170 143 6
EarI CTCTTCn'nnn_
Cuts at: 0 37 1288 1305
Size: 37 1251 17
Fragments arranged by size:
1251 37 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1099 1305
Size: 1099 206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 544 775 928 968 1305
Size: 151 393 231 153 40 337
Fragments arranged by size:
393 337 231 153 151 40
EcoO109I rG'GnC_Cy
Cuts at: 0 69 70 1033 1034 1061 1062 1187 1305
Size: 69 1 963 1 27 1 125 118
Fragments arranged by size:
963 125 118 69 27 1 1 1
HaeII r_GCGC'y
Cuts at: 0 402 450 1305
Size: 402 48 855
Fragments arranged by size:
855 402 48
KasI G'GCGC_C
Cuts at: 0 398 1305
Size: 398 907
MscI TGG'CCA
Cuts at: 0 486 1305
Size: 486 819
MslI CAynn'nnrTG
Cuts at: 0 739 1281 1305
Size: 739 542 24
Fragments arranged by size:
739 542 24
MspA1I CmG'CkG
Cuts at: 0 200 234 564 946 1103 1305
Size: 200 34 330 382 157 202
Fragments arranged by size:
382 330 202 200 157 34
NarI GG'CG_CC
Cuts at: 0 399 1305
Size: 399 906
NcoI C'CATG_G
Cuts at: 0 1095 1305
Size: 1095 210
NspI r_CATG'y
Cuts at: 0 744 1302 1305
Size: 744 558 3
Fragments arranged by size:
744 558 3
PasI CC'CwG_GG
Cuts at: 0 184 936 1305
Size: 184 752 369
Fragments arranged by size:
752 369 184
PflMI CCAn_nnn'nTGG
Cuts at: 0 104 1305
Size: 104 1201
PfoI T'CCnGG_A
Cuts at: 0 329 1305
Size: 329 976
PspOMI G'GGCC_C
Cuts at: 0 69 1033 1061 1186 1305
Size: 69 964 28 125 119
Fragments arranged by size:
964 125 119 69 28
PstI C_TGCA'G
Cuts at: 0 1075 1305
Size: 1075 230
PvuII CAG'CTG
Cuts at: 0 234 564 946 1305
Size: 234 330 382 359
Fragments arranged by size:
382 359 330 234
SacII CC_GC'GG
Cuts at: 0 201 1305
Size: 201 1104
SapI GCTCTTCn'nnn_
Cuts at: 0 1288 1305
Size: 1288 17
SfcI C'TryA_G
Cuts at: 0 222 1071 1305
Size: 222 849 234
Fragments arranged by size:
849 234 222
SfoI GGC'GCC
Cuts at: 0 400 1305
Size: 400 905
StyI C'CwwG_G
Cuts at: 0 57 428 986 1095 1305
Size: 57 371 558 109 210
Fragments arranged by size:
558 371 210 109 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 951 1305
Size: 133 818 354
Fragments arranged by size:
818 354 133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 943 1174 1305
Size: 943 231 131
Fragments arranged by size:
943 231 131
Enzymes that do cut:
AarI AfeI AleI AlfI ApaI BanI BanII BbeI
BbsI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrFI BstEII BstYI Bsu36I BtgI
BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoO109I
HaeII KasI MscI MslI MspA1I NarI NcoI NspI
PasI PflMI PfoI PspOMI PstI PvuII SacII SapI
SfcI SfoI StyI TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BlpI BmtI BpuEI BsaI BsaAI BsaBI BsaWI
BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI
DraI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI