Restriction map for
pFN21AE4417
(Linear) (Six-base) MAPSORT of: KIEE4417.seq Check: 777 from: 1 to: 1290
KIEE4417.orf
With 182 enzymes: SgfI *
March 22, 2012 17:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 489 1290
Size: 164 325 801
Fragments arranged by size:
801 325 164
AflII C'TTAA_G
Cuts at: 0 411 1290
Size: 411 879
AleI CACnn'nnGTG
Cuts at: 0 652 1290
Size: 652 638
AlwNI CAG_nnn'CTG
Cuts at: 0 92 580 1290
Size: 92 488 710
Fragments arranged by size:
710 488 92
ApaI G_GGCC'C
Cuts at: 0 972 1290
Size: 972 318
ApoI r'AATT_y
Cuts at: 0 270 310 1290
Size: 270 40 980
Fragments arranged by size:
980 270 40
AseI AT'TA_AT
Cuts at: 0 806 1290
Size: 806 484
AvaI C'yCGr_G
Cuts at: 0 31 54 1290
Size: 31 23 1236
Fragments arranged by size:
1236 31 23
BanI G'GyrC_C
Cuts at: 0 193 1290
Size: 193 1097
BanII G_rGCy'C
Cuts at: 0 972 1290
Size: 972 318
BclI T'GATC_A
Cuts at: 0 1181 1290
Size: 1181 109
BglI GCCn_nnn'nGGC
Cuts at: 0 70 1290
Size: 70 1220
BglII A'GATC_T
Cuts at: 0 926 1290
Size: 926 364
Bme1580I G_kGCm'C
Cuts at: 0 972 1290
Size: 972 318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 425 1227 1290
Size: 104 321 802 63
Fragments arranged by size:
802 321 104 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1069 1290
Size: 1069 221
BsaI GGTCTCn'nnnn_
Cuts at: 0 1080 1290
Size: 1080 210
BsaBI GATnn'nnATC
Cuts at: 0 931 1290
Size: 931 359
BsaWI w'CCGG_w
Cuts at: 0 666 1290
Size: 666 624
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 1290
Size: 406 884
BsiEI CG_ry'CG
Cuts at: 0 21 1290
Size: 21 1269
Bsp1286I G_dGCh'C
Cuts at: 0 972 1290
Size: 972 318
BspEI T'CCGG_A
Cuts at: 0 666 1290
Size: 666 624
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 296 1290
Size: 296 994
BsrBI CCG'CTC
Cuts at: 0 1176 1290
Size: 1176 114
BsrFI r'CCGG_y
Cuts at: 0 371 1290
Size: 371 919
BstEII G'GTnAC_C
Cuts at: 0 1236 1290
Size: 1236 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 654 840 1251 1290
Size: 654 186 411 39
Fragments arranged by size:
654 411 186 39
BstYI r'GATC_y
Cuts at: 0 123 926 1290
Size: 123 803 364
Fragments arranged by size:
803 364 123
BtgI C'CryG_G
Cuts at: 0 68 647 1290
Size: 68 579 643
Fragments arranged by size:
643 579 68
EaeI y'GGCC_r
Cuts at: 0 18 369 1215 1290
Size: 18 351 846 75
Fragments arranged by size:
846 351 75 18
EagI C'GGCC_G
Cuts at: 0 18 1290
Size: 18 1272
EarI CTCTTCn'nnn_
Cuts at: 0 358 1290
Size: 358 932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 164 425 489 1227 1290
Size: 104 60 261 64 738 63
Fragments arranged by size:
738 261 104 64 63 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 153 1087 1290
Size: 153 934 203
Fragments arranged by size:
934 203 153
EcoO109I rG'GnC_Cy
Cuts at: 0 115 968 1290
Size: 115 853 322
Fragments arranged by size:
853 322 115
HaeII r_GCGC'y
Cuts at: 0 581 1290
Size: 581 709
MfeI C'AATT_G
Cuts at: 0 1107 1290
Size: 1107 183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1103 1290
Size: 974 129 187
Fragments arranged by size:
974 187 129
MslI CAynn'nnrTG
Cuts at: 0 652 1057 1201 1225 1290
Size: 652 405 144 24 65
Fragments arranged by size:
652 405 144 65 24
MspA1I CmG'CkG
Cuts at: 0 70 1290
Size: 70 1220
NdeI CA'TA_TG
Cuts at: 0 828 1290
Size: 828 462
NspI r_CATG'y
Cuts at: 0 522 631 1062 1140 1290
Size: 522 109 431 78 150
Fragments arranged by size:
522 431 150 109 78
PasI CC'CwG_GG
Cuts at: 0 339 1290
Size: 339 951
PflMI CCAn_nnn'nTGG
Cuts at: 0 92 1290
Size: 92 1198
PpuMI rG'GwC_Cy
Cuts at: 0 115 1290
Size: 115 1175
PshAI GACnn'nnGTC
Cuts at: 0 662 1290
Size: 662 628
PspOMI G'GGCC_C
Cuts at: 0 968 1290
Size: 968 322
PstI C_TGCA'G
Cuts at: 0 330 1290
Size: 330 960
SacII CC_GC'GG
Cuts at: 0 71 1290
Size: 71 1219
SfcI C'TryA_G
Cuts at: 0 326 1290
Size: 326 964
SmaI CCC'GGG
Cuts at: 0 33 56 1290
Size: 33 23 1234
Fragments arranged by size:
1234 33 23
SmlI C'TyrA_G
Cuts at: 0 411 1084 1290
Size: 411 673 206
Fragments arranged by size:
673 411 206
SphI G_CATG'C
Cuts at: 0 522 631 1062 1290
Size: 522 109 431 228
Fragments arranged by size:
522 431 228 109
StyI C'CwwG_G
Cuts at: 0 738 1290
Size: 738 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 331 513 1117 1290
Size: 331 182 604 173
Fragments arranged by size:
604 331 182 173
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 300 1290
Size: 300 990
XmaI C'CCGG_G
Cuts at: 0 31 54 1290
Size: 31 23 1236
Fragments arranged by size:
1236 31 23
Enzymes that do cut:
AcuI AflII AleI AlwNI ApaI ApoI AseI AvaI
BanI BanII BclI BglI BglII Bme1580I BpmI BpuEI
BsaI BsaBI BsaWI BsgI BsiEI Bsp1286I BspEI BspMI
BsrBI BsrFI BstEII BstXI BstYI BtgI EaeI EagI
EarI Eco57MI EcoNI EcoO109I HaeII MfeI MmeI MslI
MspA1I NdeI NspI PasI PflMI PpuMI PshAI PspOMI
PstI SacII SfcI SmaI SmlI SphI StyI TaqII
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AlfI AloI ApaLI AscI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BfrBI BlpI
BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaHI BsaXI
BseRI BseYI BsiHKAI BsiWI BsmI BsmBI BspHI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MluI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI
XmnI ZraI