Restriction map for
pFN21AE4414
(Linear) (Six-base) MAPSORT of: KIEE4414.seq Check: 8591 from: 1 to: 1281
KIEE4414.orf
With 182 enzymes: SgfI *
February 2, 2012 14:09 ..
AccI GT'mk_AC
Cuts at: 0 919 1281
Size: 919 362
AfeI AGC'GCT
Cuts at: 0 49 449 1281
Size: 49 400 832
Fragments arranged by size:
832 400 49
AflIII A'CryG_T
Cuts at: 0 1046 1281
Size: 1046 235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 853 1281
Size: 853 428
AlwNI CAG_nnn'CTG
Cuts at: 0 103 1152 1281
Size: 103 1049 129
Fragments arranged by size:
1049 129 103
ApaI G_GGCC'C
Cuts at: 0 373 1173 1281
Size: 373 800 108
Fragments arranged by size:
800 373 108
ApaLI G'TGCA_C
Cuts at: 0 804 892 1281
Size: 804 88 389
Fragments arranged by size:
804 389 88
AvaI C'yCGr_G
Cuts at: 0 947 1281
Size: 947 334
BanI G'GyrC_C
Cuts at: 0 357 396 486 704 768 775 1281
Size: 357 39 90 218 64 7 506
Fragments arranged by size:
506 357 218 90 64 39 7
BanII G_rGCy'C
Cuts at: 0 373 1173 1281
Size: 373 800 108
Fragments arranged by size:
800 373 108
BbeI G_GCGC'C
Cuts at: 0 361 400 1281
Size: 361 39 881
Fragments arranged by size:
881 361 39
BglI GCCn_nnn'nGGC
Cuts at: 0 82 853 1281
Size: 82 771 428
Fragments arranged by size:
771 428 82
BlpI GC'TnA_GC
Cuts at: 0 1228 1281
Size: 1228 53
Bme1580I G_kGCm'C
Cuts at: 0 87 373 491 707 778 808 896 1173
Size: 87 286 118 216 71 30 88 277
Cuts at: 1173 1281
Size: 108
Fragments arranged by size:
286 277 216 118 108 88 87 71
30
BmgBI CAC'GTC
Cuts at: 0 765 1281
Size: 765 516
BmrI ACTGGGnnnn_n'
Cuts at: 0 710 1281
Size: 710 571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 349 830 886 1195 1281
Size: 76 273 481 56 309 86
Fragments arranged by size:
481 309 273 86 76 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 1281
Size: 54 1227
BsaAI yAC'GTr
Cuts at: 0 891 1281
Size: 891 390
BsaBI GATnn'nnATC
Cuts at: 0 733 910 1281
Size: 733 177 371
Fragments arranged by size:
733 371 177
BsaHI Gr'CG_yC
Cuts at: 0 39 358 397 515 1281
Size: 39 319 39 118 766
Fragments arranged by size:
766 319 118 39 39
BsaWI w'CCGG_w
Cuts at: 0 1073 1281
Size: 1073 208
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 1281
Size: 205 1076
BseYI C'CCAG_C
Cuts at: 0 80 1281
Size: 80 1201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 403 813 1281
Size: 334 69 410 468
Fragments arranged by size:
468 410 334 69
BsiHKAI G_wGCw'C
Cuts at: 0 157 503 808 896 1281
Size: 157 346 305 88 385
Fragments arranged by size:
385 346 305 157 88
BsmBI CGTCTCn'nnnn_
Cuts at: 0 30 481 1281
Size: 30 451 800
Fragments arranged by size:
800 451 30
Bsp1286I G_dGCh'C
Cuts at: 0 87 157 373 491 503 707 778 808
Size: 87 70 216 118 12 204 71 30
Cuts at: 808 896 1173 1281
Size: 88 277 108
Fragments arranged by size:
277 216 204 118 108 88 87 71
70 30 12
BspHI T'CATG_A
Cuts at: 0 260 902 1281
Size: 260 642 379
Fragments arranged by size:
642 379 260
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1148 1281
Size: 1148 133
BsrFI r'CCGG_y
Cuts at: 0 76 1281
Size: 76 1205
BssSI C'ACGA_G
Cuts at: 0 156 1281
Size: 156 1125
BstAPI GCAn_nnn'nTGC
Cuts at: 0 324 1281
Size: 324 957
BstYI r'GATC_y
Cuts at: 0 9 1281
Size: 9 1272
BstZ17I GTA'TAC
Cuts at: 0 920 1281
Size: 920 361
BtgI C'CryG_G
Cuts at: 0 671 848 1100 1281
Size: 671 177 252 181
Fragments arranged by size:
671 252 181 177
BtsI GCAGTG_nn'
Cuts at: 0 1232 1281
Size: 1232 49
DraIII CAC_nnn'GTG
Cuts at: 0 782 1281
Size: 782 499
EaeI y'GGCC_r
Cuts at: 0 74 421 461 584 612 674 845 1034
Size: 74 347 40 123 28 62 171 189
Cuts at: 1034 1281
Size: 247
Fragments arranged by size:
347 247 189 171 123 74 62 40
28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 559 1042 1281
Size: 559 483 239
Fragments arranged by size:
559 483 239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 349 830 886 1195 1281
Size: 76 273 481 56 309 86
Fragments arranged by size:
481 309 273 86 76 56
EcoO109I rG'GnC_Cy
Cuts at: 0 29 369 1170 1281
Size: 29 340 801 111
Fragments arranged by size:
801 340 111 29
EcoRV GAT'ATC
Cuts at: 0 912 1281
Size: 912 369
FspI TGC'GCA
Cuts at: 0 508 1281
Size: 508 773
HaeII r_GCGC'y
Cuts at: 0 51 166 298 361 400 451 551 1281
Size: 51 115 132 63 39 51 100 730
Fragments arranged by size:
730 132 115 100 63 51 51 39
HindIII A'AGCT_T
Cuts at: 0 589 1281
Size: 589 692
KasI G'GCGC_C
Cuts at: 0 357 396 1281
Size: 357 39 885
Fragments arranged by size:
885 357 39
MluI A'CGCG_T
Cuts at: 0 1046 1281
Size: 1046 235
MscI TGG'CCA
Cuts at: 0 463 586 1281
Size: 463 123 695
Fragments arranged by size:
695 463 123
MspA1I CmG'CkG
Cuts at: 0 255 402 512 943 1281
Size: 255 147 110 431 338
Fragments arranged by size:
431 338 255 147 110
NaeI GCC'GGC
Cuts at: 0 78 1281
Size: 78 1203
NarI GG'CG_CC
Cuts at: 0 358 397 1281
Size: 358 39 884
Fragments arranged by size:
884 358 39
NcoI C'CATG_G
Cuts at: 0 1100 1281
Size: 1100 181
NgoMIV G'CCGG_C
Cuts at: 0 76 1281
Size: 76 1205
NspI r_CATG'y
Cuts at: 0 130 1281
Size: 130 1151
PasI CC'CwG_GG
Cuts at: 0 1239 1281
Size: 1239 42
PflMI CCAn_nnn'nTGG
Cuts at: 0 659 1281
Size: 659 622
PfoI T'CCnGG_A
Cuts at: 0 1082 1281
Size: 1082 199
PmlI CAC'GTG
Cuts at: 0 891 1281
Size: 891 390
PspOMI G'GGCC_C
Cuts at: 0 369 1169 1281
Size: 369 800 112
Fragments arranged by size:
800 369 112
PstI C_TGCA'G
Cuts at: 0 1145 1281
Size: 1145 136
PvuII CAG'CTG
Cuts at: 0 255 1281
Size: 255 1026
SexAI A'CCwGG_T
Cuts at: 0 482 1281
Size: 482 799
SfcI C'TryA_G
Cuts at: 0 1141 1281
Size: 1141 140
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 853 1281
Size: 853 428
SfoI GGC'GCC
Cuts at: 0 359 398 1281
Size: 359 39 883
Fragments arranged by size:
883 359 39
SmlI C'TyrA_G
Cuts at: 0 33 1281
Size: 33 1248
SphI G_CATG'C
Cuts at: 0 130 1281
Size: 130 1151
StyI C'CwwG_G
Cuts at: 0 438 1100 1281
Size: 438 662 181
Fragments arranged by size:
662 438 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 280 651 808 1281
Size: 280 371 157 473
Fragments arranged by size:
473 371 280 157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 104 136 1281
Size: 104 32 1145
Fragments arranged by size:
1145 104 32
Enzymes that do cut:
AccI AfeI AflIII AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BglI BlpI Bme1580I BmgBI BmrI
BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI BssSI
BstAPI BstYI BstZ17I BtgI BtsI DraIII EaeI EciI
Eco57MI EcoO109I EcoRV FspI HaeII HindIII KasI MluI
MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PfoI PmlI PspOMI PstI PvuII SexAI SfcI
SfiI SfoI SmlI SphI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BmtI BplI Bpu10I BsaI BsaXI BsiEI BsiWI BsmI
BspEI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstXI
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI
EcoICRI EcoNI EcoRI FalI FseI FspAI Hin4I HincII
HpaI KpnI MfeI MmeI MslI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI