Restriction map for
pFN21AE4409
(Linear) (Six-base) MAPSORT of: KIEE4409.seq Check: 2794 from: 1 to: 1269
KIEE4409.orf
With 182 enzymes: SgfI *
March 22, 2012 17:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1093 1269
Size: 1093 176
Acc65I G'GTAC_C
Cuts at: 0 648 1269
Size: 648 621
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 703 1269
Size: 592 111 566
Fragments arranged by size:
592 566 111
AleI CACnn'nnGTG
Cuts at: 0 1020 1269
Size: 1020 249
AlwNI CAG_nnn'CTG
Cuts at: 0 174 186 681 736 1203 1269
Size: 174 12 495 55 467 66
Fragments arranged by size:
495 467 174 66 55 12
ApaI G_GGCC'C
Cuts at: 0 1155 1210 1269
Size: 1155 55 59
Fragments arranged by size:
1155 59 55
AvaI C'yCGr_G
Cuts at: 0 611 1269
Size: 611 658
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 632 665 1269
Size: 632 33 604
Fragments arranged by size:
632 604 33
BanI G'GyrC_C
Cuts at: 0 92 648 796 1269
Size: 92 556 148 473
Fragments arranged by size:
556 473 148 92
BanII G_rGCy'C
Cuts at: 0 628 1155 1166 1210 1269
Size: 628 527 11 44 59
Fragments arranged by size:
628 527 59 44 11
BbeI G_GCGC'C
Cuts at: 0 96 1269
Size: 96 1173
BbsI GAAGACnn'nnnn_
Cuts at: 0 243 557 1269
Size: 243 314 712
Fragments arranged by size:
712 314 243
BbvCI CC'TCA_GC
Cuts at: 0 1217 1269
Size: 1217 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 878 912 1269
Size: 878 34 357
Fragments arranged by size:
878 357 34
BclI T'GATC_A
Cuts at: 0 810 1269
Size: 810 459
BglI GCCn_nnn'nGGC
Cuts at: 0 826 1269
Size: 826 443
BlpI GC'TnA_GC
Cuts at: 0 1160 1269
Size: 1160 109
Bme1580I G_kGCm'C
Cuts at: 0 1155 1210 1269
Size: 1155 55 59
Fragments arranged by size:
1155 59 55
BmgBI CAC'GTC
Cuts at: 0 513 1269
Size: 513 756
BmrI ACTGGGnnnn_n'
Cuts at: 0 474 1073 1269
Size: 474 599 196
Fragments arranged by size:
599 474 196
BmtI G_CTAG'C
Cuts at: 0 983 1269
Size: 983 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 1058 1150 1269
Size: 160 898 92 119
Fragments arranged by size:
898 160 119 92
Bpu10I CC'TnA_GC
Cuts at: 0 953 1217 1269
Size: 953 264 52
Fragments arranged by size:
953 264 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 1269
Size: 84 1185
BsaI GGTCTCn'nnnn_
Cuts at: 0 524 1269
Size: 524 745
BsaAI yAC'GTr
Cuts at: 0 717 1125 1269
Size: 717 408 144
Fragments arranged by size:
717 408 144
BsaBI GATnn'nnATC
Cuts at: 0 599 1269
Size: 599 670
BsaHI Gr'CG_yC
Cuts at: 0 93 1269
Size: 93 1176
BsaWI w'CCGG_w
Cuts at: 0 218 1269
Size: 218 1051
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 133 163 1269
Size: 133 30 1106
Fragments arranged by size:
1106 133 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 1055 1269
Size: 157 898 214
Fragments arranged by size:
898 214 157
BseYI C'CCAG_C
Cuts at: 0 47 201 281 868 936 1269
Size: 47 154 80 587 68 333
Fragments arranged by size:
587 333 154 80 68 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1269
Size: 191 1078
BsiEI CG_ry'CG
Cuts at: 0 84 1269
Size: 84 1185
BsiHKAI G_wGCw'C
Cuts at: 0 368 389 1166 1269
Size: 368 21 777 103
Fragments arranged by size:
777 368 103 21
BsmI GAATG_Cn'
Cuts at: 0 53 1108 1269
Size: 53 1055 161
Fragments arranged by size:
1055 161 53
Bsp1286I G_dGCh'C
Cuts at: 0 368 389 628 1155 1166 1210 1269
Size: 368 21 239 527 11 44 59
Fragments arranged by size:
527 368 239 59 44 21 11
BspEI T'CCGG_A
Cuts at: 0 218 1269
Size: 218 1051
BspHI T'CATG_A
Cuts at: 0 591 813 1269
Size: 591 222 456
Fragments arranged by size:
591 456 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1093 1269
Size: 1093 176
BsrBI CCG'CTC
Cuts at: 0 224 1269
Size: 224 1045
BstXI CCAn_nnnn'nTGG
Cuts at: 0 376 1269
Size: 376 893
BstYI r'GATC_y
Cuts at: 0 29 1200 1269
Size: 29 1171 69
Fragments arranged by size:
1171 69 29
BtgI C'CryG_G
Cuts at: 0 879 1269
Size: 879 390
BtsI GCAGTG_nn'
Cuts at: 0 243 1015 1269
Size: 243 772 254
Fragments arranged by size:
772 254 243
DraIII CAC_nnn'GTG
Cuts at: 0 454 1269
Size: 454 815
EarI CTCTTCn'nnn_
Cuts at: 0 1123 1269
Size: 1123 146
EcoICRI GAG'CTC
Cuts at: 0 1164 1269
Size: 1164 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 592 703 1058 1150 1269
Size: 160 432 111 355 92 119
Fragments arranged by size:
432 355 160 119 111 92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 571 1269
Size: 571 698
EcoO109I rG'GnC_Cy
Cuts at: 0 1151 1269
Size: 1151 118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 27 1269
Size: 27 1242
HaeII r_GCGC'y
Cuts at: 0 96 1269
Size: 96 1173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 14 46 816 848 1269
Size: 14 32 770 32 421
Fragments arranged by size:
770 421 32 32 14
HindIII A'AGCT_T
Cuts at: 0 464 1269
Size: 464 805
KasI G'GCGC_C
Cuts at: 0 92 1269
Size: 92 1177
KpnI G_GTAC'C
Cuts at: 0 652 1269
Size: 652 617
MslI CAynn'nnrTG
Cuts at: 0 326 374 410 1020 1269
Size: 326 48 36 610 249
Fragments arranged by size:
610 326 249 48 36
MspA1I CmG'CkG
Cuts at: 0 205 1269
Size: 205 1064
NarI GG'CG_CC
Cuts at: 0 93 1269
Size: 93 1176
NheI G'CTAG_C
Cuts at: 0 979 1269
Size: 979 290
NspI r_CATG'y
Cuts at: 0 543 1174 1269
Size: 543 631 95
Fragments arranged by size:
631 543 95
PflMI CCAn_nnn'nTGG
Cuts at: 0 1203 1269
Size: 1203 66
PfoI T'CCnGG_A
Cuts at: 0 269 1269
Size: 269 1000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 856 888 1269
Size: 856 32 381
Fragments arranged by size:
856 381 32
PspOMI G'GGCC_C
Cuts at: 0 1151 1206 1269
Size: 1151 55 63
Fragments arranged by size:
1151 63 55
PvuII CAG'CTG
Cuts at: 0 205 1269
Size: 205 1064
SacI G_AGCT'C
Cuts at: 0 1166 1269
Size: 1166 103
SfcI C'TryA_G
Cuts at: 0 773 1269
Size: 773 496
SfoI GGC'GCC
Cuts at: 0 94 1269
Size: 94 1175
SmlI C'TyrA_G
Cuts at: 0 63 1269
Size: 63 1206
StuI AGG'CCT
Cuts at: 0 925 1269
Size: 925 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 560 1269
Size: 560 709
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 133 165 200 232 481 513 1065 1097
Size: 133 32 35 32 249 32 552 32
Cuts at: 1097 1269
Size: 172
Fragments arranged by size:
552 249 172 133 35 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1086 1269
Size: 1086 183
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI ApaI AvaI BaeI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BstXI BstYI BtgI BtsI DraIII EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KasI
KpnI MslI MspA1I NarI NheI NspI PflMI PfoI
PpiI PspOMI PvuII SacI SfcI SfoI SmlI StuI
TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BciVI BfrBI BglII BplI BsiWI BsmBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EaeI EagI
EciI EcoRI EcoRV FseI FspI FspAI HincII HpaI
MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StyI SwaI TatI Tth111I XbaI XhoI XmaI XmnI
ZraI