(Linear) (Six-base) MAPSORT of: KIEE4406.seq  Check: 6373  from: 1  to: 1263



KIEE4406.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:55  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     190     487     499    1263 
   Size:        190     297      12     764

  Fragments arranged by size: 

                 764     297     190      12
 
ApoI r'AATT_y

Cuts at:      0     139     160     375     689    1214    1263 
   Size:        139      21     215     314     525      49

  Fragments arranged by size: 

                 525     314     215     139      49      21
 
AseI AT'TA_AT

Cuts at:      0     554    1263 
   Size:        554     709
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     744    1263 
   Size:        744     519
 
BclI T'GATC_A

Cuts at:      0     414    1263 
   Size:        414     849
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     178    1263 
   Size:        178    1085
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     509    1103    1263 
   Size:        509     594     160

  Fragments arranged by size: 

                 594     509     160
 
BsaBI GATnn'nnATC

Cuts at:      0     626    1263 
   Size:        626     637
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     466    1263 
   Size:        466     797
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1138    1263 
   Size:       1138     125
 
BsiHKAI G_wGCw'C

Cuts at:      0     947    1263 
   Size:        947     316
 
Bsp1286I G_dGCh'C

Cuts at:      0     947    1263 
   Size:        947     316
 
BsrDI GCAATG_nn'

Cuts at:      0     824     986    1140    1263 
   Size:        824     162     154     123

  Fragments arranged by size: 

                 824     162     154     123
 
BsrGI T'GTAC_A

Cuts at:      0     346    1263 
   Size:        346     917
 
BstBI TT'CG_AA

Cuts at:      0     755    1263 
   Size:        755     508
 
BstYI r'GATC_y

Cuts at:      0     627    1263 
   Size:        627     636
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      33     741    1263 
   Size:         33     708     522

  Fragments arranged by size: 

                 708     522      33
 
EarI CTCTTCn'nnn_

Cuts at:      0     231    1263 
   Size:        231    1032
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     509    1103    1263 
   Size:        509     594     160

  Fragments arranged by size: 

                 594     509     160
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     491    1263 
   Size:        491     772
 
EcoO109I rG'GnC_Cy

Cuts at:      0      30    1263 
   Size:         30    1233
 
EcoRI G'AATT_C

Cuts at:      0     689    1263 
   Size:        689     574
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     938    1263 
   Size:        938     325
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     161     193     734     766    1263 
   Size:        161      32     541      32     497

  Fragments arranged by size: 

                 541     497     161      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     846    1263 
   Size:        846     417
 
MslI CAynn'nnrTG

Cuts at:      0     184     462    1263 
   Size:        184     278     801

  Fragments arranged by size: 

                 801     278     184
 
MspA1I CmG'CkG

Cuts at:      0       7    1263 
   Size:          7    1256
 
PpuMI rG'GwC_Cy

Cuts at:      0      30    1263 
   Size:         30    1233
 
PstI C_TGCA'G

Cuts at:      0      28     195    1263 
   Size:         28     167    1068

  Fragments arranged by size: 

                1068     167      28
 
SapI GCTCTTCn'nnn_

Cuts at:      0     231    1263 
   Size:        231    1032
 
SfcI C'TryA_G

Cuts at:      0      24     191     845    1157    1263 
   Size:         24     167     654     312     106

  Fragments arranged by size: 

                 654     312     167     106      24
 
TatI w'GTAC_w

Cuts at:      0     346    1253    1263 
   Size:        346     907      10

  Fragments arranged by size: 

                 907     346      10
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     255     287     653     685    1263 
   Size:        255      32     366      32     578

  Fragments arranged by size: 

                 578     366     255      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     906    1263 
   Size:        906     357
 
XmnI GAAnn'nnTTC

Cuts at:      0      42     922    1263 
   Size:         42     880     341

  Fragments arranged by size: 

                 880     341      42
 

 Enzymes that do cut:

    AlwNI     ApoI     AseI     BbsI     BclI     BmrI     BpmI    BsaBI
    BseRI     BsgI  BsiHKAI Bsp1286I    BsrDI    BsrGI    BstBI    BstYI
    BtgZI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I
     MmeI     MslI   MspA1I    PpuMI     PstI     SapI     SfcI     TatI
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI
     ZraI