Restriction map for
pFN21AE4405
(Linear) (Six-base) MAPSORT of: KIEE4405.seq Check: 7589 from: 1 to: 1257
KIEE4405.orf
With 182 enzymes: SgfI *
March 22, 2012 17:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 138 1257
Size: 138 1119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 881 1257
Size: 675 206 376
Fragments arranged by size:
675 376 206
AlwNI CAG_nnn'CTG
Cuts at: 0 129 1257
Size: 129 1128
ApaI G_GGCC'C
Cuts at: 0 159 1257
Size: 159 1098
ApoI r'AATT_y
Cuts at: 0 519 530 612 1107 1171 1257
Size: 519 11 82 495 64 86
Fragments arranged by size:
519 495 86 82 64 11
AvaI C'yCGr_G
Cuts at: 0 27 133 160 1257
Size: 27 106 27 1097
Fragments arranged by size:
1097 106 27 27
BanII G_rGCy'C
Cuts at: 0 34 159 1257
Size: 34 125 1098
Fragments arranged by size:
1098 125 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 1106 1257
Size: 1106 151
BbvCI CC'TCA_GC
Cuts at: 0 18 1257
Size: 18 1239
BciVI GTATCCnnnnn_n'
Cuts at: 0 867 1257
Size: 867 390
BglI GCCn_nnn'nGGC
Cuts at: 0 38 1257
Size: 38 1219
BglII A'GATC_T
Cuts at: 0 1114 1257
Size: 1114 143
Bme1580I G_kGCm'C
Cuts at: 0 159 1257
Size: 159 1098
Bpu10I CC'TnA_GC
Cuts at: 0 18 1257
Size: 18 1239
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 1257
Size: 806 451
BsaI GGTCTCn'nnnn_
Cuts at: 0 893 1257
Size: 893 364
BsaWI w'CCGG_w
Cuts at: 0 1181 1257
Size: 1181 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 1257
Size: 265 30 962
Fragments arranged by size:
962 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 121 178 181 184 187 190 193
Size: 88 33 57 3 3 3 3 3
Cuts at: 193 196 962 1257
Size: 3 766 295
Fragments arranged by size:
766 295 88 57 33 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 929 1257
Size: 929 328
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1257
Size: 166 1091
BsiEI CG_ry'CG
Cuts at: 0 279 1257
Size: 279 978
BsmI GAATG_Cn'
Cuts at: 0 124 1257
Size: 124 1133
Bsp1286I G_dGCh'C
Cuts at: 0 34 159 1257
Size: 34 125 1098
Fragments arranged by size:
1098 125 34
BspHI T'CATG_A
Cuts at: 0 504 1257
Size: 504 753
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 138 1257
Size: 138 1119
BsrBI CCG'CTC
Cuts at: 0 281 1257
Size: 281 976
BsrFI r'CCGG_y
Cuts at: 0 223 1044 1257
Size: 223 821 213
Fragments arranged by size:
821 223 213
BstBI TT'CG_AA
Cuts at: 0 116 1257
Size: 116 1141
BstYI r'GATC_y
Cuts at: 0 1114 1257
Size: 1114 143
BtgI C'CryG_G
Cuts at: 0 34 215 1257
Size: 34 181 1042
Fragments arranged by size:
1042 181 34
DraI TTT'AAA
Cuts at: 0 570 1257
Size: 570 687
DrdI GACnn_nn'nnGTC
Cuts at: 0 338 1257
Size: 338 919
EaeI y'GGCC_r
Cuts at: 0 200 276 1257
Size: 200 76 981
Fragments arranged by size:
981 200 76
EagI C'GGCC_G
Cuts at: 0 276 1257
Size: 276 981
EarI CTCTTCn'nnn_
Cuts at: 0 954 999 1257
Size: 954 45 258
Fragments arranged by size:
954 258 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1257
Size: 19 1238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 881 1257
Size: 675 206 376
Fragments arranged by size:
675 376 206
EcoO109I rG'GnC_Cy
Cuts at: 0 155 156 299 1257
Size: 155 1 143 958
Fragments arranged by size:
958 155 143 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 265 297 639 671 1257
Size: 1 32 232 32 342 32 586
Fragments arranged by size:
586 342 232 32 32 32 1
MfeI C'AATT_G
Cuts at: 0 479 1257
Size: 479 778
MspA1I CmG'CkG
Cuts at: 0 22 36 778 1257
Size: 22 14 742 479
Fragments arranged by size:
742 479 22 14
NaeI GCC'GGC
Cuts at: 0 225 1257
Size: 225 1032
NdeI CA'TA_TG
Cuts at: 0 373 620 1257
Size: 373 247 637
Fragments arranged by size:
637 373 247
NgoMIV G'CCGG_C
Cuts at: 0 223 1257
Size: 223 1034
PacI TTA_AT'TAA
Cuts at: 0 527 1257
Size: 527 730
PflMI CCAn_nnn'nTGG
Cuts at: 0 708 1257
Size: 708 549
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 1257
Size: 97 32 1128
Fragments arranged by size:
1128 97 32
PpuMI rG'GwC_Cy
Cuts at: 0 299 1257
Size: 299 958
PshAI GACnn'nnGTC
Cuts at: 0 1189 1257
Size: 1189 68
PsiI TTA'TAA
Cuts at: 0 1169 1257
Size: 1169 88
PspOMI G'GGCC_C
Cuts at: 0 155 1257
Size: 155 1102
PspXI vC'TCGA_Gb
Cuts at: 0 133 1257
Size: 133 1124
SacII CC_GC'GG
Cuts at: 0 37 1257
Size: 37 1220
SapI GCTCTTCn'nnn_
Cuts at: 0 999 1257
Size: 999 258
SfcI C'TryA_G
Cuts at: 0 539 1257
Size: 539 718
SmlI C'TyrA_G
Cuts at: 0 133 821 1257
Size: 133 688 436
Fragments arranged by size:
688 436 133
SspI AAT'ATT
Cuts at: 0 599 1257
Size: 599 658
StyI C'CwwG_G
Cuts at: 0 449 1257
Size: 449 808
TatI w'GTAC_w
Cuts at: 0 979 1257
Size: 979 278
XhoI C'TCGA_G
Cuts at: 0 133 1257
Size: 133 1124
XmnI GAAnn'nnTTC
Cuts at: 0 901 1257
Size: 901 356
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApoI AvaI BanII BbsI
BbvCI BciVI BglI BglII Bme1580I Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmI Bsp1286I
BspHI BspMI BsrBI BsrFI BstBI BstYI BtgI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I Hin4I
MfeI MspA1I NaeI NdeI NgoMIV PacI PflMI PpiI
PpuMI PshAI PsiI PspOMI PspXI SacII SapI SfcI
SmlI SspI StyI TatI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BcgI BclI BfrBI
BlpI BmgBI BmrI BmtI BplI BpmI BsaAI BsaBI
BsaHI BsiHKAI BsiWI BsmBI BspEI BsrDI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraIII EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI NarI NcoI NheI
NotI NruI NsiI NspI PasI PciI PfoI PmeI
PmlI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XmaI ZraI