Restriction map for
pFN21AE4404
(Linear) (Six-base) MAPSORT of: KIEE4404.seq Check: 1165 from: 1 to: 1257
KIEE4404.orf
With 182 enzymes: SgfI *
March 22, 2012 17:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 812 1257
Size: 812 445
AatII G_ACGT'C
Cuts at: 0 614 1257
Size: 614 643
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 235 909 1066 1257
Size: 49 186 674 157 191
Fragments arranged by size:
674 191 186 157 49
AflIII A'CryG_T
Cuts at: 0 1146 1257
Size: 1146 111
AleI CACnn'nnGTG
Cuts at: 0 230 511 1257
Size: 230 281 746
Fragments arranged by size:
746 281 230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 772 1257
Size: 772 485
AlwNI CAG_nnn'CTG
Cuts at: 0 588 752 831 1257
Size: 588 164 79 426
Fragments arranged by size:
588 426 164 79
ApaI G_GGCC'C
Cuts at: 0 692 1257
Size: 692 565
ApoI r'AATT_y
Cuts at: 0 286 564 619 1257
Size: 286 278 55 638
Fragments arranged by size:
638 286 278 55
AvaI C'yCGr_G
Cuts at: 0 164 461 1029 1257
Size: 164 297 568 228
Fragments arranged by size:
568 297 228 164
AvrII C'CTAG_G
Cuts at: 0 909 1257
Size: 909 348
BanI G'GyrC_C
Cuts at: 0 131 1116 1257
Size: 131 985 141
Fragments arranged by size:
985 141 131
BanII G_rGCy'C
Cuts at: 0 520 692 1257
Size: 520 172 565
Fragments arranged by size:
565 520 172
BbeI G_GCGC'C
Cuts at: 0 135 1257
Size: 135 1122
BbsI GAAGACnn'nnnn_
Cuts at: 0 499 1257
Size: 499 758
BclI T'GATC_A
Cuts at: 0 143 1257
Size: 143 1114
BlpI GC'TnA_GC
Cuts at: 0 190 760 808 1257
Size: 190 570 48 449
Fragments arranged by size:
570 449 190 48
Bme1580I G_kGCm'C
Cuts at: 0 79 424 692 1257
Size: 79 345 268 565
Fragments arranged by size:
565 345 268 79
BmgBI CAC'GTC
Cuts at: 0 670 1257
Size: 670 587
BmrI ACTGGGnnnn_n'
Cuts at: 0 121 886 1257
Size: 121 765 371
Fragments arranged by size:
765 371 121
BmtI G_CTAG'C
Cuts at: 0 472 1257
Size: 472 785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 759 760 862 1257
Size: 472 287 1 102 395
Fragments arranged by size:
472 395 287 102 1
Bpu10I CC'TnA_GC
Cuts at: 0 1125 1257
Size: 1125 132
BsaAI yAC'GTr
Cuts at: 0 1056 1257
Size: 1056 201
BsaHI Gr'CG_yC
Cuts at: 0 132 528 611 1257
Size: 132 396 83 646
Fragments arranged by size:
646 396 132 83
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 372 402 1257
Size: 372 30 855
Fragments arranged by size:
855 372 30
BseYI C'CCAG_C
Cuts at: 0 295 730 1257
Size: 295 435 527
Fragments arranged by size:
527 435 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1207 1257
Size: 1207 50
BsiHKAI G_wGCw'C
Cuts at: 0 746 1257
Size: 746 511
BsmI GAATG_Cn'
Cuts at: 0 124 656 1257
Size: 124 532 601
Fragments arranged by size:
601 532 124
BsmBI CGTCTCn'nnnn_
Cuts at: 0 602 1257
Size: 602 655
Bsp1286I G_dGCh'C
Cuts at: 0 79 424 520 692 746 1257
Size: 79 345 96 172 54 511
Fragments arranged by size:
511 345 172 96 79 54
BspHI T'CATG_A
Cuts at: 0 983 1257
Size: 983 274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 686 812 1257
Size: 686 126 445
Fragments arranged by size:
686 445 126
BsrFI r'CCGG_y
Cuts at: 0 551 1257
Size: 551 706
BsrGI T'GTAC_A
Cuts at: 0 503 1257
Size: 503 754
ClaI AT'CG_AT
Cuts at: 0 626 1257
Size: 626 631
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 264 299 1257
Size: 264 35 958
Fragments arranged by size:
958 264 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 601 1257
Size: 601 656
EaeI y'GGCC_r
Cuts at: 0 264 1257
Size: 264 993
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 235 472 759 760 862 909 1066
Size: 49 186 237 287 1 102 47 157
Cuts at: 1066 1257
Size: 191
Fragments arranged by size:
287 237 191 186 157 102 49 47
1
EcoO109I rG'GnC_Cy
Cuts at: 0 689 1257
Size: 689 568
EcoRI G'AATT_C
Cuts at: 0 564 619 1257
Size: 564 55 638
Fragments arranged by size:
638 564 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 456 1257
Size: 456 801
HaeII r_GCGC'y
Cuts at: 0 135 1257
Size: 135 1122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 372 404 1089 1121 1257
Size: 372 32 685 32 136
Fragments arranged by size:
685 372 136 32 32
HincII GTy'rAC
Cuts at: 0 1002 1257
Size: 1002 255
KasI G'GCGC_C
Cuts at: 0 131 1257
Size: 131 1126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1257
Size: 132 1125
MslI CAynn'nnrTG
Cuts at: 0 230 260 511 1257
Size: 230 30 251 746
Fragments arranged by size:
746 251 230 30
MspA1I CmG'CkG
Cuts at: 0 511 1080 1257
Size: 511 569 177
Fragments arranged by size:
569 511 177
NaeI GCC'GGC
Cuts at: 0 553 1257
Size: 553 704
NarI GG'CG_CC
Cuts at: 0 132 1257
Size: 132 1125
NgoMIV G'CCGG_C
Cuts at: 0 551 1257
Size: 551 706
NheI G'CTAG_C
Cuts at: 0 468 1257
Size: 468 789
NspI r_CATG'y
Cuts at: 0 1150 1257
Size: 1150 107
PasI CC'CwG_GG
Cuts at: 0 417 1257
Size: 417 840
PciI A'CATG_T
Cuts at: 0 1146 1257
Size: 1146 111
PfoI T'CCnGG_A
Cuts at: 0 449 614 839 1257
Size: 449 165 225 418
Fragments arranged by size:
449 418 225 165
PspOMI G'GGCC_C
Cuts at: 0 688 1257
Size: 688 569
PvuII CAG'CTG
Cuts at: 0 511 1080 1257
Size: 511 569 177
Fragments arranged by size:
569 511 177
SfcI C'TryA_G
Cuts at: 0 1220 1257
Size: 1220 37
SfoI GGC'GCC
Cuts at: 0 133 1257
Size: 133 1124
SgrAI Cr'CCGG_yG
Cuts at: 0 551 1257
Size: 551 706
SmaI CCC'GGG
Cuts at: 0 1031 1257
Size: 1031 226
SmlI C'TyrA_G
Cuts at: 0 164 461 1257
Size: 164 297 796
Fragments arranged by size:
796 297 164
StuI AGG'CCT
Cuts at: 0 908 1257
Size: 908 349
StyI C'CwwG_G
Cuts at: 0 391 903 909 944 1257
Size: 391 512 6 35 313
Fragments arranged by size:
512 391 313 35 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 58 443 611 806 1257
Size: 58 385 168 195 451
Fragments arranged by size:
451 385 195 168 58
TatI w'GTAC_w
Cuts at: 0 503 1257
Size: 503 754
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 140 172 1257
Size: 140 32 1085
Fragments arranged by size:
1085 140 32
XhoI C'TCGA_G
Cuts at: 0 164 461 1257
Size: 164 297 796
Fragments arranged by size:
796 297 164
XmaI C'CCGG_G
Cuts at: 0 1029 1257
Size: 1029 228
ZraI GAC'GTC
Cuts at: 0 612 1257
Size: 612 645
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI AvrII BanI BanII BbeI BbsI BclI
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaAI
BsaHI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrFI BsrGI ClaI CspCI DrdI EaeI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
MmeI MslI MspA1I NaeI NarI NgoMIV NheI NspI
PasI PciI PfoI PspOMI PvuII SfcI SfoI SgrAI
SmaI SmlI StuI StyI TaqII TatI TstI XhoI
XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI BaeI BamHI BbvCI BcgI
BciVI BfrBI BglI BglII BplI BpuEI BsaI BsaBI
BsaWI BseRI BsiEI BsiWI BspEI BsrBI BsrDI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII EagI EarI EciI
EcoICRI EcoNI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MscI NcoI NdeI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XcmI XmnI