Restriction map for
pFN21AE4403
(Linear) (Six-base) MAPSORT of: KIEE4403.seq Check: 1576 from: 1 to: 1257
KIEE4403.orf
With 182 enzymes: SgfI *
March 22, 2012 17:55 ..
Acc65I G'GTAC_C
Cuts at: 0 86 1257
Size: 86 1171
AflIII A'CryG_T
Cuts at: 0 709 1257
Size: 709 548
AhdI GACnn_n'nnGTC
Cuts at: 0 339 1257
Size: 339 918
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 875 907 1257
Size: 875 32 350
Fragments arranged by size:
875 350 32
AvaI C'yCGr_G
Cuts at: 0 488 542 1257
Size: 488 54 715
Fragments arranged by size:
715 488 54
AvrII C'CTAG_G
Cuts at: 0 133 930 1257
Size: 133 797 327
Fragments arranged by size:
797 327 133
BamHI G'GATC_C
Cuts at: 0 995 1257
Size: 995 262
BanI G'GyrC_C
Cuts at: 0 64 86 1224 1257
Size: 64 22 1138 33
Fragments arranged by size:
1138 64 33 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 476 1257
Size: 476 781
BciVI GTATCCnnnnn_n'
Cuts at: 0 928 1257
Size: 928 329
BfrBI ATG'CAT
Cuts at: 0 439 1257
Size: 439 818
BlpI GC'TnA_GC
Cuts at: 0 520 1006 1257
Size: 520 486 251
Fragments arranged by size:
520 486 251
Bme1580I G_kGCm'C
Cuts at: 0 67 1257
Size: 67 1190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 545 1257
Size: 545 712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 515 607 1036 1257
Size: 299 216 92 429 221
Fragments arranged by size:
429 299 221 216 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1257
Size: 580 677
BsaAI yAC'GTr
Cuts at: 0 477 1257
Size: 477 780
BsaBI GATnn'nnATC
Cuts at: 0 1159 1257
Size: 1159 98
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 901 931 1257
Size: 901 30 326
Fragments arranged by size:
901 326 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 352 808 1257
Size: 352 456 449
Fragments arranged by size:
456 449 352
BseYI C'CCAG_C
Cuts at: 0 715 905 1257
Size: 715 190 352
Fragments arranged by size:
715 352 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 1257
Size: 522 735
BsiEI CG_ry'CG
Cuts at: 0 1091 1257
Size: 1091 166
Bsp1286I G_dGCh'C
Cuts at: 0 67 1257
Size: 67 1190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 98 176 1257
Size: 98 78 1081
Fragments arranged by size:
1081 98 78
BsrDI GCAATG_nn'
Cuts at: 0 42 1257
Size: 42 1215
BsrFI r'CCGG_y
Cuts at: 0 98 1257
Size: 98 1159
BstAPI GCAn_nnn'nTGC
Cuts at: 0 477 1257
Size: 477 780
BstBI TT'CG_AA
Cuts at: 0 955 1257
Size: 955 302
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1060 1257
Size: 1060 197
BstYI r'GATC_y
Cuts at: 0 429 995 1031 1257
Size: 429 566 36 226
Fragments arranged by size:
566 429 226 36
BtgI C'CryG_G
Cuts at: 0 59 513 1085 1257
Size: 59 454 572 172
Fragments arranged by size:
572 454 172 59
BtsI GCAGTG_nn'
Cuts at: 0 578 1079 1257
Size: 578 501 178
Fragments arranged by size:
578 501 178
ClaI AT'CG_AT
Cuts at: 0 665 1163 1257
Size: 665 498 94
Fragments arranged by size:
665 498 94
EaeI y'GGCC_r
Cuts at: 0 2 30 191 294 501 572 612 733
Size: 2 28 161 103 207 71 40 121
Cuts at: 733 1257
Size: 524
Fragments arranged by size:
524 207 161 121 103 71 40 28
2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 515 607 1036 1257
Size: 299 216 92 429 221
Fragments arranged by size:
429 299 221 216 92
EcoO109I rG'GnC_Cy
Cuts at: 0 591 603 1014 1257
Size: 591 12 411 243
Fragments arranged by size:
591 411 243 12
FspI TGC'GCA
Cuts at: 0 172 496 1257
Size: 172 324 761
Fragments arranged by size:
761 324 172
FspAI rTGC'GCAy
Cuts at: 0 496 1257
Size: 496 761
HaeII r_GCGC'y
Cuts at: 0 930 1257
Size: 930 327
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 968 1000 1257
Size: 325 32 611 32 257
Fragments arranged by size:
611 325 257 32 32
HindIII A'AGCT_T
Cuts at: 0 223 1257
Size: 223 1034
KpnI G_GTAC'C
Cuts at: 0 90 1257
Size: 90 1167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1136 1181 1257
Size: 1136 45 76
Fragments arranged by size:
1136 76 45
MscI TGG'CCA
Cuts at: 0 4 296 574 735 1257
Size: 4 292 278 161 522
Fragments arranged by size:
522 292 278 161 4
MslI CAynn'nnrTG
Cuts at: 0 761 1257
Size: 761 496
MspA1I CmG'CkG
Cuts at: 0 657 1087 1257
Size: 657 430 170
Fragments arranged by size:
657 430 170
NcoI C'CATG_G
Cuts at: 0 59 1257
Size: 59 1198
NsiI A_TGCA'T
Cuts at: 0 441 1257
Size: 441 816
NspI r_CATG'y
Cuts at: 0 713 1257
Size: 713 544
PasI CC'CwG_GG
Cuts at: 0 407 1257
Size: 407 850
PciI A'CATG_T
Cuts at: 0 709 1257
Size: 709 548
PfoI T'CCnGG_A
Cuts at: 0 122 1034 1257
Size: 122 912 223
Fragments arranged by size:
912 223 122
PpuMI rG'GwC_Cy
Cuts at: 0 603 1014 1257
Size: 603 411 243
Fragments arranged by size:
603 411 243
SacII CC_GC'GG
Cuts at: 0 1088 1257
Size: 1088 169
SexAI A'CCwGG_T
Cuts at: 0 1178 1257
Size: 1178 79
SmaI CCC'GGG
Cuts at: 0 544 1257
Size: 544 713
SmlI C'TyrA_G
Cuts at: 0 595 1257
Size: 595 662
StyI C'CwwG_G
Cuts at: 0 59 133 453 930 960 1257
Size: 59 74 320 477 30 297
Fragments arranged by size:
477 320 297 74 59 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 258 466 1257
Size: 258 208 791
Fragments arranged by size:
791 258 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 711 743 901 933 1257
Size: 711 32 158 32 324
Fragments arranged by size:
711 324 158 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 373 1257
Size: 373 884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 582 1257
Size: 582 675
XmaI C'CCGG_G
Cuts at: 0 542 1257
Size: 542 715
Enzymes that do cut:
Acc65I AflIII AhdI AloI AvaI AvrII BamHI BanI
BbsI BciVI BfrBI BlpI Bme1580I BplI BpmI BpuEI
BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I
BspMI BsrDI BsrFI BstAPI BstBI BstXI BstYI BtgI
BtsI ClaI EaeI Eco57MI EcoO109I FspI FspAI HaeII
Hin4I HindIII KpnI MmeI MscI MslI MspA1I NcoI
NsiI NspI PasI PciI PfoI PpuMI SacII SexAI
SmaI SmlI StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI BaeI BanII BbeI BbvCI BcgI BclI BglI
BglII BmgBI BmrI BmtI Bpu10I BsaI BsaHI BsaWI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstEII BstZ17I Bsu36I BtgZI CspCI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI HincII HpaI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
XbaI XhoI XmnI ZraI