Restriction map for
pFN21AE4401
(Linear) (Six-base) MAPSORT of: KIEE4401.seq Check: 4017 from: 1 to: 1251
KIEE4401.orf
With 182 enzymes: SgfI *
March 22, 2012 17:54 ..
AccI GT'mk_AC
Cuts at: 0 713 1166 1251
Size: 713 453 85
Fragments arranged by size:
713 453 85
AleI CACnn'nnGTG
Cuts at: 0 560 1251
Size: 560 691
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 487 1251
Size: 487 764
AlwNI CAG_nnn'CTG
Cuts at: 0 390 852 1251
Size: 390 462 399
Fragments arranged by size:
462 399 390
AvaI C'yCGr_G
Cuts at: 0 935 1024 1251
Size: 935 89 227
Fragments arranged by size:
935 227 89
BamHI G'GATC_C
Cuts at: 0 568 1251
Size: 568 683
BanI G'GyrC_C
Cuts at: 0 328 658 1251
Size: 328 330 593
Fragments arranged by size:
593 330 328
BbeI G_GCGC'C
Cuts at: 0 332 1251
Size: 332 919
BglI GCCn_nnn'nGGC
Cuts at: 0 97 118 425 847 1104 1251
Size: 97 21 307 422 257 147
Fragments arranged by size:
422 307 257 147 97 21
BglII A'GATC_T
Cuts at: 0 866 1251
Size: 866 385
Bme1580I G_kGCm'C
Cuts at: 0 294 1135 1146 1251
Size: 294 841 11 105
Fragments arranged by size:
841 294 105 11
BmgBI CAC'GTC
Cuts at: 0 215 315 341 1251
Size: 215 100 26 910
Fragments arranged by size:
910 215 100 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 151 597 1251
Size: 151 446 654
Fragments arranged by size:
654 446 151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 420 574 1251
Size: 420 154 677
Fragments arranged by size:
677 420 154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 415 774 910 1251
Size: 24 391 359 136 341
Fragments arranged by size:
391 359 341 136 24
BsaHI Gr'CG_yC
Cuts at: 0 329 1096 1163 1251
Size: 329 767 67 88
Fragments arranged by size:
767 329 88 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 136 166 1251
Size: 136 30 1085
Fragments arranged by size:
1085 136 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 222 286 769 1251
Size: 118 104 64 483 482
Fragments arranged by size:
483 482 118 104 64
BseYI C'CCAG_C
Cuts at: 0 693 834 884 1244 1251
Size: 693 141 50 360 7
Fragments arranged by size:
693 360 141 50 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 721 1251
Size: 181 540 530
Fragments arranged by size:
540 530 181
BsiHKAI G_wGCw'C
Cuts at: 0 232 555 1251
Size: 232 323 696
Fragments arranged by size:
696 323 232
Bsp1286I G_dGCh'C
Cuts at: 0 232 294 555 1135 1146 1251
Size: 232 62 261 580 11 105
Fragments arranged by size:
580 261 232 105 62 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 153 1251
Size: 153 1098
BsrBI CCG'CTC
Cuts at: 0 621 1251
Size: 621 630
BsrDI GCAATG_nn'
Cuts at: 0 606 960 972 1251
Size: 606 354 12 279
Fragments arranged by size:
606 354 279 12
BsrFI r'CCGG_y
Cuts at: 0 515 655 841 1251
Size: 515 140 186 410
Fragments arranged by size:
515 410 186 140
BstEII G'GTnAC_C
Cuts at: 0 1239 1251
Size: 1239 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 457 1236 1251
Size: 457 779 15
Fragments arranged by size:
779 457 15
BstYI r'GATC_y
Cuts at: 0 568 866 1196 1251
Size: 568 298 330 55
Fragments arranged by size:
568 330 298 55
Bsu36I CC'TnA_GG
Cuts at: 0 130 286 1251
Size: 130 156 965
Fragments arranged by size:
965 156 130
BtgI C'CryG_G
Cuts at: 0 476 523 662 681 717 1251
Size: 476 47 139 19 36 534
Fragments arranged by size:
534 476 139 47 36 19
BtsI GCAGTG_nn'
Cuts at: 0 62 1251
Size: 62 1189
EaeI y'GGCC_r
Cuts at: 0 18 437 459 520 843 906 1251
Size: 18 419 22 61 323 63 345
Fragments arranged by size:
419 345 323 63 61 22 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 415 774 910 1251
Size: 24 391 359 136 341
Fragments arranged by size:
391 359 341 136 24
EcoO109I rG'GnC_Cy
Cuts at: 0 71 630 741 849 1251
Size: 71 559 111 108 402
Fragments arranged by size:
559 402 111 108 71
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 553 1251
Size: 553 698
HaeII r_GCGC'y
Cuts at: 0 332 974 1251
Size: 332 642 277
Fragments arranged by size:
642 332 277
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 560 592 1072 1104 1251
Size: 560 32 480 32 147
Fragments arranged by size:
560 480 147 32 32
KasI G'GCGC_C
Cuts at: 0 328 1251
Size: 328 923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 1251
Size: 266 985
MscI TGG'CCA
Cuts at: 0 908 1251
Size: 908 343
MslI CAynn'nnrTG
Cuts at: 0 406 560 1251
Size: 406 154 691
Fragments arranged by size:
691 406 154
MspA1I CmG'CkG
Cuts at: 0 150 266 693 838 1058 1232 1251
Size: 150 116 427 145 220 174 19
Fragments arranged by size:
427 220 174 150 145 116 19
NaeI GCC'GGC
Cuts at: 0 843 1251
Size: 843 408
NarI GG'CG_CC
Cuts at: 0 329 1251
Size: 329 922
NgoMIV G'CCGG_C
Cuts at: 0 841 1251
Size: 841 410
PflMI CCAn_nnn'nTGG
Cuts at: 0 852 1251
Size: 852 399
PfoI T'CCnGG_A
Cuts at: 0 614 926 1187 1251
Size: 614 312 261 64
Fragments arranged by size:
614 312 261 64
PpuMI rG'GwC_Cy
Cuts at: 0 71 630 741 1251
Size: 71 559 111 510
Fragments arranged by size:
559 510 111 71
PspXI vC'TCGA_Gb
Cuts at: 0 935 1251
Size: 935 316
PstI C_TGCA'G
Cuts at: 0 692 1251
Size: 692 559
PvuII CAG'CTG
Cuts at: 0 266 693 838 1251
Size: 266 427 145 413
Fragments arranged by size:
427 413 266 145
SanDI GG'GwC_CC
Cuts at: 0 71 741 1251
Size: 71 670 510
Fragments arranged by size:
670 510 71
ScaI AGT'ACT
Cuts at: 0 901 1251
Size: 901 350
SfcI C'TryA_G
Cuts at: 0 688 1251
Size: 688 563
SfoI GGC'GCC
Cuts at: 0 330 1251
Size: 330 921
SmlI C'TyrA_G
Cuts at: 0 935 1251
Size: 935 316
StuI AGG'CCT
Cuts at: 0 673 1251
Size: 673 578
StyI C'CwwG_G
Cuts at: 0 47 1251
Size: 47 1204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 397 579 1251
Size: 397 182 672
Fragments arranged by size:
672 397 182
TatI w'GTAC_w
Cuts at: 0 899 1251
Size: 899 352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 199 231 1251
Size: 199 32 1020
Fragments arranged by size:
1020 199 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 48 1251
Size: 48 1203
XhoI C'TCGA_G
Cuts at: 0 935 1251
Size: 935 316
Enzymes that do cut:
AccI AleI AlfI AlwNI AvaI BamHI BanI BbeI
BglI BglII Bme1580I BmgBI BmrI BplI BpmI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI
EaeI Eco57MI EcoO109I FalI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV PflMI PfoI
PpuMI PspXI PstI PvuII SanDI ScaI SfcI SfoI
SmlI StuI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BlpI BmtI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XmaI XmnI ZraI