Restriction map for
pFN21AE4392
(Linear) (Six-base) MAPSORT of: KIEE4392.seq Check: 2722 from: 1 to: 1245
KIEE4392.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
Acc65I G'GTAC_C
Cuts at: 0 1057 1245
Size: 1057 188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 1032 1245
Size: 599 433 213
Fragments arranged by size:
599 433 213
AleI CACnn'nnGTG
Cuts at: 0 580 1245
Size: 580 665
AlwNI CAG_nnn'CTG
Cuts at: 0 41 168 964 1245
Size: 41 127 796 281
Fragments arranged by size:
796 281 127 41
BanI G'GyrC_C
Cuts at: 0 709 1057 1245
Size: 709 348 188
Fragments arranged by size:
709 348 188
BanII G_rGCy'C
Cuts at: 0 604 809 938 1245
Size: 604 205 129 307
Fragments arranged by size:
604 307 205 129
BbsI GAAGACnn'nnnn_
Cuts at: 0 675 981 1245
Size: 675 306 264
Fragments arranged by size:
675 306 264
BbvCI CC'TCA_GC
Cuts at: 0 281 694 1245
Size: 281 413 551
Fragments arranged by size:
551 413 281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 87 121 252 286 1245
Size: 87 34 131 34 959
Fragments arranged by size:
959 131 87 34 34
BclI T'GATC_A
Cuts at: 0 702 1245
Size: 702 543
BglI GCCn_nnn'nGGC
Cuts at: 0 1103 1245
Size: 1103 142
Bme1580I G_kGCm'C
Cuts at: 0 265 1245
Size: 265 980
BmrI ACTGGGnnnn_n'
Cuts at: 0 14 1094 1245
Size: 14 1080 151
Fragments arranged by size:
1080 151 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1227 1245
Size: 1227 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 688 1147 1245
Size: 561 127 459 98
Fragments arranged by size:
561 459 127 98
Bpu10I CC'TnA_GC
Cuts at: 0 281 694 932 1245
Size: 281 413 238 313
Fragments arranged by size:
413 313 281 238
BsaI GGTCTCn'nnnn_
Cuts at: 0 368 1245
Size: 368 877
BsaBI GATnn'nnATC
Cuts at: 0 779 1245
Size: 779 466
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 237 267 348 378 953 983 1245
Size: 237 30 81 30 575 30 262
Fragments arranged by size:
575 262 237 81 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 682 1162 1245
Size: 682 480 83
Fragments arranged by size:
682 480 83
BseYI C'CCAG_C
Cuts at: 0 16 41 1002 1160 1245
Size: 16 25 961 158 85
Fragments arranged by size:
961 158 85 25 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 1245
Size: 181 1064
BsiHKAI G_wGCw'C
Cuts at: 0 477 547 809 938 1245
Size: 477 70 262 129 307
Fragments arranged by size:
477 307 262 129 70
BsmI GAATG_Cn'
Cuts at: 0 796 1245
Size: 796 449
Bsp1286I G_dGCh'C
Cuts at: 0 265 477 547 604 809 938 1245
Size: 265 212 70 57 205 129 307
Fragments arranged by size:
307 265 212 205 129 70 57
BsrBI CCG'CTC
Cuts at: 0 200 240 672 1245
Size: 200 40 432 573
Fragments arranged by size:
573 432 200 40
BsrDI GCAATG_nn'
Cuts at: 0 1059 1245
Size: 1059 186
BsrFI r'CCGG_y
Cuts at: 0 484 1245
Size: 484 761
BssSI C'ACGA_G
Cuts at: 0 937 1245
Size: 937 308
BstEII G'GTnAC_C
Cuts at: 0 171 1245
Size: 171 1074
BtgI C'CryG_G
Cuts at: 0 49 176 256 341 628 657 1214 1245
Size: 49 127 80 85 287 29 557 31
Fragments arranged by size:
557 287 127 85 80 49 31 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 309 446 713 1053 1245
Size: 309 137 267 340 192
Fragments arranged by size:
340 309 267 192 137
BtsI GCAGTG_nn'
Cuts at: 0 855 861 1245
Size: 855 6 384
Fragments arranged by size:
855 384 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 172 207 1245
Size: 172 35 1038
Fragments arranged by size:
1038 172 35
EaeI y'GGCC_r
Cuts at: 0 654 1090 1217 1245
Size: 654 436 127 28
Fragments arranged by size:
654 436 127 28
EarI CTCTTCn'nnn_
Cuts at: 0 272 1245
Size: 272 973
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1083 1245
Size: 1083 162
EcoICRI GAG'CTC
Cuts at: 0 807 936 1245
Size: 807 129 309
Fragments arranged by size:
807 309 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 599 688 1032 1147 1245
Size: 561 38 89 344 115 98
Fragments arranged by size:
561 344 115 98 89 38
EcoO109I rG'GnC_Cy
Cuts at: 0 908 1096 1155 1245
Size: 908 188 59 90
Fragments arranged by size:
908 188 90 59
HaeII r_GCGC'y
Cuts at: 0 28 723 1245
Size: 28 695 522
Fragments arranged by size:
695 522 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 953 985 1245
Size: 953 32 260
Fragments arranged by size:
953 260 32
KpnI G_GTAC'C
Cuts at: 0 1061 1245
Size: 1061 184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1245
Size: 1022 223
MscI TGG'CCA
Cuts at: 0 656 1092 1219 1245
Size: 656 436 127 26
Fragments arranged by size:
656 436 127 26
MslI CAynn'nnrTG
Cuts at: 0 580 1213 1245
Size: 580 633 32
Fragments arranged by size:
633 580 32
MspA1I CmG'CkG
Cuts at: 0 51 168 258 621 740 1245
Size: 51 117 90 363 119 505
Fragments arranged by size:
505 363 119 117 90 51
NaeI GCC'GGC
Cuts at: 0 486 1245
Size: 486 759
NcoI C'CATG_G
Cuts at: 0 176 341 657 1245
Size: 176 165 316 588
Fragments arranged by size:
588 316 176 165
NgoMIV G'CCGG_C
Cuts at: 0 484 1245
Size: 484 761
PfoI T'CCnGG_A
Cuts at: 0 29 538 1245
Size: 29 509 707
Fragments arranged by size:
707 509 29
PshAI GACnn'nnGTC
Cuts at: 0 152 1245
Size: 152 1093
PstI C_TGCA'G
Cuts at: 0 960 1245
Size: 960 285
SacI G_AGCT'C
Cuts at: 0 809 938 1245
Size: 809 129 307
Fragments arranged by size:
809 307 129
SacII CC_GC'GG
Cuts at: 0 52 259 1245
Size: 52 207 986
Fragments arranged by size:
986 207 52
SfcI C'TryA_G
Cuts at: 0 75 368 956 965 1245
Size: 75 293 588 9 280
Fragments arranged by size:
588 293 280 75 9
StyI C'CwwG_G
Cuts at: 0 134 176 341 414 657 1245
Size: 134 42 165 73 243 588
Fragments arranged by size:
588 243 165 134 73 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 75 142 183 1245
Size: 75 67 41 1062
Fragments arranged by size:
1062 75 67 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 237 269 348 380 1245
Size: 237 32 79 32 865
Fragments arranged by size:
865 237 79 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 679 1245
Size: 679 566
XmnI GAAnn'nnTTC
Cuts at: 0 148 1245
Size: 148 1097
Enzymes that do cut:
Acc65I AcuI AleI AlwNI BanI BanII BbsI BbvCI
BcgI BclI BglI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BsrBI BsrDI BsrFI BssSI BstEII BtgI BtgZI
BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
HaeII Hin4I KpnI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV PfoI PshAI PstI SacI SacII SfcI
StyI TaqII TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BciVI BfrBI
BglII BlpI BmgBI BmtI BpuEI BsaAI BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrGI BssHII
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I ClaI DraI
DraIII DrdI EagI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI MfeI MluI
NarI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XcmI XhoI XmaI ZraI