Restriction map for
pFN21AE4389
(Linear) (Six-base) MAPSORT of: KIEE4389.seq Check: 2441 from: 1 to: 1224
KIEE4389.orf
With 182 enzymes: SgfI *
March 22, 2012 17:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1224
Size: 403 821
AgeI A'CCGG_T
Cuts at: 0 398 1224
Size: 398 826
AleI CACnn'nnGTG
Cuts at: 0 244 1105 1224
Size: 244 861 119
Fragments arranged by size:
861 244 119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1123 1224
Size: 1123 101
AlwNI CAG_nnn'CTG
Cuts at: 0 57 363 708 1224
Size: 57 306 345 516
Fragments arranged by size:
516 345 306 57
AvaI C'yCGr_G
Cuts at: 0 16 1224
Size: 16 1208
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 45 78 1224
Size: 45 33 1146
Fragments arranged by size:
1146 45 33
BanI G'GyrC_C
Cuts at: 0 162 199 249 328 831 878 1224
Size: 162 37 50 79 503 47 346
Fragments arranged by size:
503 346 162 79 50 47 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 249 1224
Size: 249 975
BciVI GTATCCnnnnn_n'
Cuts at: 0 73 1224
Size: 73 1151
BglI GCCn_nnn'nGGC
Cuts at: 0 134 502 1224
Size: 134 368 722
Fragments arranged by size:
722 368 134
Bme1580I G_kGCm'C
Cuts at: 0 333 1224
Size: 333 891
BmrI ACTGGGnnnn_n'
Cuts at: 0 611 1208 1224
Size: 611 597 16
Fragments arranged by size:
611 597 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1224
Size: 1033 191
BsaWI w'CCGG_w
Cuts at: 0 398 834 1224
Size: 398 436 390
Fragments arranged by size:
436 398 390
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 86 116 1224
Size: 86 30 1108
Fragments arranged by size:
1108 86 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 37 130 207 390 454 874 1224
Size: 34 3 93 77 183 64 420 350
Fragments arranged by size:
420 350 183 93 77 64 34 3
BseYI C'CCAG_C
Cuts at: 0 132 1071 1224
Size: 132 939 153
Fragments arranged by size:
939 153 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 640 1099 1224
Size: 496 144 459 125
Fragments arranged by size:
496 459 144 125
BsiHKAI G_wGCw'C
Cuts at: 0 29 1224
Size: 29 1195
BsmI GAATG_Cn'
Cuts at: 0 682 1224
Size: 682 542
Bsp1286I G_dGCh'C
Cuts at: 0 29 333 1224
Size: 29 304 891
Fragments arranged by size:
891 304 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 65 804 1224
Size: 65 739 420
Fragments arranged by size:
739 420 65
BsrBI CCG'CTC
Cuts at: 0 570 1224
Size: 570 654
BsrFI r'CCGG_y
Cuts at: 0 398 1224
Size: 398 826
BstAPI GCAn_nnn'nTGC
Cuts at: 0 511 708 1224
Size: 511 197 516
Fragments arranged by size:
516 511 197
BstEII G'GTnAC_C
Cuts at: 0 177 451 591 900 1224
Size: 177 274 140 309 324
Fragments arranged by size:
324 309 274 177 140
BstXI CCAn_nnnn'nTGG
Cuts at: 0 889 1224
Size: 889 335
Bsu36I CC'TnA_GG
Cuts at: 0 499 1224
Size: 499 725
BtgI C'CryG_G
Cuts at: 0 1155 1224
Size: 1155 69
EaeI y'GGCC_r
Cuts at: 0 193 1224
Size: 193 1031
EarI CTCTTCn'nnn_
Cuts at: 0 49 1224
Size: 49 1175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 1224
Size: 37 1187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1033 1224
Size: 403 630 191
Fragments arranged by size:
630 403 191
EcoNI CCTnn'n_nnAGG
Cuts at: 0 520 730 1224
Size: 520 210 494
Fragments arranged by size:
520 494 210
EcoO109I rG'GnC_Cy
Cuts at: 0 13 1068 1224
Size: 13 1055 156
Fragments arranged by size:
1055 156 13
HaeII r_GCGC'y
Cuts at: 0 812 1224
Size: 812 412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 1224
Size: 16 32 1176
Fragments arranged by size:
1176 32 16
HincII GTy'rAC
Cuts at: 0 188 1224
Size: 188 1036
MscI TGG'CCA
Cuts at: 0 195 1224
Size: 195 1029
MslI CAynn'nnrTG
Cuts at: 0 242 244 418 1105 1224
Size: 242 2 174 687 119
Fragments arranged by size:
687 242 174 119 2
MspA1I CmG'CkG
Cuts at: 0 132 480 867 889 1224
Size: 132 348 387 22 335
Fragments arranged by size:
387 348 335 132 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 363 462 1224
Size: 363 99 762
Fragments arranged by size:
762 363 99
PfoI T'CCnGG_A
Cuts at: 0 686 1224
Size: 686 538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1132 1164 1224
Size: 1132 32 60
Fragments arranged by size:
1132 60 32
PpuMI rG'GwC_Cy
Cuts at: 0 13 1068 1224
Size: 13 1055 156
Fragments arranged by size:
1055 156 13
PstI C_TGCA'G
Cuts at: 0 713 872 1224
Size: 713 159 352
Fragments arranged by size:
713 352 159
PvuII CAG'CTG
Cuts at: 0 132 867 889 1224
Size: 132 735 22 335
Fragments arranged by size:
735 335 132 22
SanDI GG'GwC_CC
Cuts at: 0 13 1068 1224
Size: 13 1055 156
Fragments arranged by size:
1055 156 13
SapI GCTCTTCn'nnn_
Cuts at: 0 49 1224
Size: 49 1175
SfcI C'TryA_G
Cuts at: 0 709 868 1224
Size: 709 159 356
Fragments arranged by size:
709 356 159
StuI AGG'CCT
Cuts at: 0 292 1224
Size: 292 932
StyI C'CwwG_G
Cuts at: 0 456 964 1224
Size: 456 508 260
Fragments arranged by size:
508 456 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 44 573 1104 1224
Size: 44 529 531 120
Fragments arranged by size:
531 529 120 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 86 118 328 360 1224
Size: 86 32 210 32 864
Fragments arranged by size:
864 210 86 32 32
Enzymes that do cut:
AcuI AgeI AleI AlfI AlwNI AvaI BaeI BanI
BbsI BciVI BglI Bme1580I BmrI BpmI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrFI BstAPI BstEII BstXI Bsu36I BtgI EaeI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MscI
MslI MspA1I PflMI PfoI PpiI PpuMI PstI PvuII
SanDI SapI SfcI StuI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BanII BbeI BbvCI BcgI BclI BfrBI
BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstYI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI