Restriction map for
pFN21AE4386
(Linear) (Six-base) MAPSORT of: KIEE4386.seq Check: 8991 from: 1 to: 1218
KIEE4386.orf
With 182 enzymes: SgfI *
March 22, 2012 17:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 410 1066 1218
Size: 410 656 152
Fragments arranged by size:
656 410 152
AfeI AGC'GCT
Cuts at: 0 66 1218
Size: 66 1152
AflII C'TTAA_G
Cuts at: 0 1120 1218
Size: 1120 98
AleI CACnn'nnGTG
Cuts at: 0 52 878 1218
Size: 52 826 340
Fragments arranged by size:
826 340 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 929 1218
Size: 929 289
AlwNI CAG_nnn'CTG
Cuts at: 0 162 402 417 570 1218
Size: 162 240 15 153 648
Fragments arranged by size:
648 240 162 153 15
ApaLI G'TGCA_C
Cuts at: 0 871 1218
Size: 871 347
ApoI r'AATT_y
Cuts at: 0 407 1218
Size: 407 811
AvaI C'yCGr_G
Cuts at: 0 496 1218
Size: 496 722
BamHI G'GATC_C
Cuts at: 0 344 747 1218
Size: 344 403 471
Fragments arranged by size:
471 403 344
BanI G'GyrC_C
Cuts at: 0 212 383 686 937 1054 1218
Size: 212 171 303 251 117 164
Fragments arranged by size:
303 251 212 171 164 117
BbeI G_GCGC'C
Cuts at: 0 216 690 941 1218
Size: 216 474 251 277
Fragments arranged by size:
474 277 251 216
BbsI GAAGACnn'nnnn_
Cuts at: 0 1147 1218
Size: 1147 71
BglI GCCn_nnn'nGGC
Cuts at: 0 431 695 733 935 1218
Size: 431 264 38 202 283
Fragments arranged by size:
431 283 264 202 38
BlpI GC'TnA_GC
Cuts at: 0 196 1218
Size: 196 1022
Bme1580I G_kGCm'C
Cuts at: 0 875 1218
Size: 875 343
BmrI ACTGGGnnnn_n'
Cuts at: 0 846 1218
Size: 846 372
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 26 1218
Size: 26 1192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 409 796 1058 1218
Size: 226 183 387 262 160
Fragments arranged by size:
387 262 226 183 160
Bpu10I CC'TnA_GC
Cuts at: 0 68 217 1218
Size: 68 149 1001
Fragments arranged by size:
1001 149 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 1218
Size: 310 908
BsaI GGTCTCn'nnnn_
Cuts at: 0 629 1218
Size: 629 589
BsaHI Gr'CG_yC
Cuts at: 0 213 687 938 1218
Size: 213 474 251 280
Fragments arranged by size:
474 280 251 213
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1078 1108 1218
Size: 1078 30 110
Fragments arranged by size:
1078 110 30
BseYI C'CCAG_C
Cuts at: 0 371 506 768 933 955 1218
Size: 371 135 262 165 22 263
Fragments arranged by size:
371 263 262 165 135 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 526 844 899 1218
Size: 103 423 318 55 319
Fragments arranged by size:
423 319 318 103 55
BsiEI CG_ry'CG
Cuts at: 0 20 1218
Size: 20 1198
BsiHKAI G_wGCw'C
Cuts at: 0 12 875 1218
Size: 12 863 343
Fragments arranged by size:
863 343 12
Bsp1286I G_dGCh'C
Cuts at: 0 12 875 1218
Size: 12 863 343
Fragments arranged by size:
863 343 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 410 1066 1218
Size: 410 656 152
Fragments arranged by size:
656 410 152
BsrBI CCG'CTC
Cuts at: 0 5 210 1094 1218
Size: 5 205 884 124
Fragments arranged by size:
884 205 124 5
BsrFI r'CCGG_y
Cuts at: 0 545 713 925 1218
Size: 545 168 212 293
Fragments arranged by size:
545 293 212 168
BssHII G'CGCG_C
Cuts at: 0 200 1218
Size: 200 1018
BstAPI GCAn_nnn'nTGC
Cuts at: 0 402 549 1218
Size: 402 147 669
Fragments arranged by size:
669 402 147
BstYI r'GATC_y
Cuts at: 0 344 747 1218
Size: 344 403 471
Fragments arranged by size:
471 403 344
Bsu36I CC'TnA_GG
Cuts at: 0 296 617 1218
Size: 296 321 601
Fragments arranged by size:
601 321 296
BtgI C'CryG_G
Cuts at: 0 753 920 1196 1218
Size: 753 167 276 22
Fragments arranged by size:
753 276 167 22
BtsI GCAGTG_nn'
Cuts at: 0 911 1165 1218
Size: 911 254 53
Fragments arranged by size:
911 254 53
DraIII CAC_nnn'GTG
Cuts at: 0 366 1218
Size: 366 852
DrdI GACnn_nn'nnGTC
Cuts at: 0 298 1218
Size: 298 920
EaeI y'GGCC_r
Cuts at: 0 17 153 467 818 923 927 1080 1218
Size: 17 136 314 351 105 4 153 138
Fragments arranged by size:
351 314 153 138 136 105 17 4
EagI C'GGCC_G
Cuts at: 0 17 1218
Size: 17 1201
EarI CTCTTCn'nnn_
Cuts at: 0 361 1218
Size: 361 857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 89 222 617 1218
Size: 89 133 395 601
Fragments arranged by size:
601 395 133 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 409 796 1058 1218
Size: 226 183 387 262 160
Fragments arranged by size:
387 262 226 183 160
EcoO109I rG'GnC_Cy
Cuts at: 0 300 1218
Size: 300 918
EcoRI G'AATT_C
Cuts at: 0 407 1218
Size: 407 811
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1149 1218
Size: 1149 69
FseI GG_CCGG'CC
Cuts at: 0 929 1218
Size: 929 289
HaeII r_GCGC'y
Cuts at: 0 68 216 690 941 1218
Size: 68 148 474 251 277
Fragments arranged by size:
474 277 251 148 68
HincII GTy'rAC
Cuts at: 0 516 1218
Size: 516 702
HindIII A'AGCT_T
Cuts at: 0 1117 1218
Size: 1117 101
KasI G'GCGC_C
Cuts at: 0 212 686 937 1218
Size: 212 474 251 281
Fragments arranged by size:
474 281 251 212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 1094 1218
Size: 37 1057 124
Fragments arranged by size:
1057 124 37
MscI TGG'CCA
Cuts at: 0 469 1218
Size: 469 749
MslI CAynn'nnrTG
Cuts at: 0 52 878 1180 1218
Size: 52 826 302 38
Fragments arranged by size:
826 302 52 38
MspA1I CmG'CkG
Cuts at: 0 195 381 828 933 959 1110 1218
Size: 195 186 447 105 26 151 108
Fragments arranged by size:
447 195 186 151 108 105 26
NaeI GCC'GGC
Cuts at: 0 927 1218
Size: 927 291
NarI GG'CG_CC
Cuts at: 0 213 687 938 1218
Size: 213 474 251 280
Fragments arranged by size:
474 280 251 213
NcoI C'CATG_G
Cuts at: 0 1196 1218
Size: 1196 22
NgoMIV G'CCGG_C
Cuts at: 0 925 1218
Size: 925 293
PasI CC'CwG_GG
Cuts at: 0 554 719 1218
Size: 554 165 499
Fragments arranged by size:
554 499 165
PflMI CCAn_nnn'nTGG
Cuts at: 0 366 465 1197 1218
Size: 366 99 732 21
Fragments arranged by size:
732 366 99 21
PfoI T'CCnGG_A
Cuts at: 0 437 1218
Size: 437 781
PpuMI rG'GwC_Cy
Cuts at: 0 300 1218
Size: 300 918
PstI C_TGCA'G
Cuts at: 0 149 317 863 908 1063 1172 1218
Size: 149 168 546 45 155 109 46
Fragments arranged by size:
546 168 155 149 109 46 45
PvuII CAG'CTG
Cuts at: 0 195 828 933 1218
Size: 195 633 105 285
Fragments arranged by size:
633 285 195 105
ScaI AGT'ACT
Cuts at: 0 1087 1218
Size: 1087 131
SfcI C'TryA_G
Cuts at: 0 145 313 859 897 904 1059 1168 1218
Size: 145 168 546 38 7 155 109 50
Fragments arranged by size:
546 168 155 145 109 50 38 7
SfoI GGC'GCC
Cuts at: 0 214 688 939 1218
Size: 214 474 251 279
Fragments arranged by size:
474 279 251 214
SmlI C'TyrA_G
Cuts at: 0 325 1120 1218
Size: 325 795 98
Fragments arranged by size:
795 325 98
StyI C'CwwG_G
Cuts at: 0 1196 1218
Size: 1196 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 868 969 1218
Size: 868 101 249
Fragments arranged by size:
868 249 101
TatI w'GTAC_w
Cuts at: 0 1085 1218
Size: 1085 133
Enzymes that do cut:
AarI AfeI AflII AleI AlfI AlwNI ApaLI ApoI
AvaI BamHI BanI BbeI BbsI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BssHII BstAPI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FseI HaeII HincII HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI
PpuMI PstI PvuII ScaI SfcI SfoI SmlI StyI
TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvrII BaeI BanII
BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
BsaAI BsaBI BsaWI BseRI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI EcoICRI EcoNI EcoRV FspI
FspAI Hin4I HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI