Restriction map for
pFN21AE4382
(Linear) (Six-base) MAPSORT of: KIEE4382.seq Check: 5753 from: 1 to: 1209
KIEE4382.orf
With 182 enzymes: SgfI *
March 22, 2012 17:53 ..
AatII G_ACGT'C
Cuts at: 0 824 1209
Size: 824 385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1124 1183 1209
Size: 1124 59 26
Fragments arranged by size:
1124 59 26
AhdI GACnn_n'nnGTC
Cuts at: 0 219 1209
Size: 219 990
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 436 468 1209
Size: 436 32 741
Fragments arranged by size:
741 436 32
ApoI r'AATT_y
Cuts at: 0 1155 1209
Size: 1155 54
BamHI G'GATC_C
Cuts at: 0 411 1209
Size: 411 798
BbsI GAAGACnn'nnnn_
Cuts at: 0 767 1112 1171 1209
Size: 767 345 59 38
Fragments arranged by size:
767 345 59 38
BclI T'GATC_A
Cuts at: 0 201 1209
Size: 201 1008
BfrBI ATG'CAT
Cuts at: 0 254 1209
Size: 254 955
BlpI GC'TnA_GC
Cuts at: 0 193 1209
Size: 193 1016
BmgBI CAC'GTC
Cuts at: 0 238 1209
Size: 238 971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 1157 1209
Size: 859 298 52
Fragments arranged by size:
859 298 52
BsaI GGTCTCn'nnnn_
Cuts at: 0 501 1023 1209
Size: 501 522 186
Fragments arranged by size:
522 501 186
BsaHI Gr'CG_yC
Cuts at: 0 821 998 1209
Size: 821 177 211
Fragments arranged by size:
821 211 177
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1041 1209
Size: 1041 168
BseYI C'CCAG_C
Cuts at: 0 722 828 1209
Size: 722 106 381
Fragments arranged by size:
722 381 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 1209
Size: 809 400
BsiHKAI G_wGCw'C
Cuts at: 0 925 1209
Size: 925 284
Bsp1286I G_dGCh'C
Cuts at: 0 925 1209
Size: 925 284
BsrDI GCAATG_nn'
Cuts at: 0 122 566 1209
Size: 122 444 643
Fragments arranged by size:
643 444 122
BstXI CCAn_nnnn'nTGG
Cuts at: 0 351 1209
Size: 351 858
BstYI r'GATC_y
Cuts at: 0 411 1209
Size: 411 798
Bsu36I CC'TnA_GG
Cuts at: 0 1024 1209
Size: 1024 185
BtgI C'CryG_G
Cuts at: 0 260 1209
Size: 260 949
BtsI GCAGTG_nn'
Cuts at: 0 792 1209
Size: 792 417
EaeI y'GGCC_r
Cuts at: 0 156 1209
Size: 156 1053
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 1124 1157 1183 1209
Size: 859 265 33 26 26
Fragments arranged by size:
859 265 33 26 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1198 1209
Size: 1198 11
HaeII r_GCGC'y
Cuts at: 0 231 598 1209
Size: 231 367 611
Fragments arranged by size:
611 367 231
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 1020 1052 1209
Size: 205 32 783 32 157
Fragments arranged by size:
783 205 157 32 32
HindIII A'AGCT_T
Cuts at: 0 989 1209
Size: 989 220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 1209
Size: 581 628
MscI TGG'CCA
Cuts at: 0 158 1209
Size: 158 1051
MslI CAynn'nnrTG
Cuts at: 0 50 259 270 290 656 1209
Size: 50 209 11 20 366 553
Fragments arranged by size:
553 366 209 50 20 11
MspA1I CmG'CkG
Cuts at: 0 520 1209
Size: 520 689
NcoI C'CATG_G
Cuts at: 0 260 1209
Size: 260 949
NdeI CA'TA_TG
Cuts at: 0 267 1209
Size: 267 942
NsiI A_TGCA'T
Cuts at: 0 256 1209
Size: 256 953
NspI r_CATG'y
Cuts at: 0 192 862 1209
Size: 192 670 347
Fragments arranged by size:
670 347 192
PfoI T'CCnGG_A
Cuts at: 0 22 875 1209
Size: 22 853 334
Fragments arranged by size:
853 334 22
PsiI TTA'TAA
Cuts at: 0 107 1209
Size: 107 1102
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 736 768 1209
Size: 736 32 441
Fragments arranged by size:
736 441 32
PstI C_TGCA'G
Cuts at: 0 478 1209
Size: 478 731
PvuII CAG'CTG
Cuts at: 0 520 1209
Size: 520 689
SfcI C'TryA_G
Cuts at: 0 474 1209
Size: 474 735
SspI AAT'ATT
Cuts at: 0 318 1209
Size: 318 891
StyI C'CwwG_G
Cuts at: 0 260 1209
Size: 260 949
TatI w'GTAC_w
Cuts at: 0 1055 1177 1209
Size: 1055 122 32
Fragments arranged by size:
1055 122 32
Tth111I GACn'n_nGTC
Cuts at: 0 385 1209
Size: 385 824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 844 1209
Size: 844 365
ZraI GAC'GTC
Cuts at: 0 822 1209
Size: 822 387
Enzymes that do cut:
AatII AcuI AhdI AloI ApoI BamHI BbsI BclI
BfrBI BlpI BmgBI BpmI BsaI BsaHI BseRI BseYI
BsgI BsiHKAI Bsp1286I BsrDI BstXI BstYI Bsu36I BtgI
BtsI EaeI Eco57MI EcoNI HaeII Hin4I HindIII MmeI
MscI MslI MspA1I NcoI NdeI NsiI NspI PfoI
PsiI PsrI PstI PvuII SfcI SspI StyI TatI
Tth111I XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BanII BbeI BbvCI BcgI
BciVI BglI BglII Bme1580I BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TaqII TstI XbaI XhoI XmaI
XmnI