Restriction map for
pFN21AE4377
(Linear) (Six-base) MAPSORT of: KIEE4377.seq Check: 5579 from: 1 to: 1197
KIEE4377.orf
With 182 enzymes: SgfI *
March 22, 2012 17:52 ..
AatII G_ACGT'C
Cuts at: 0 1058 1197
Size: 1058 139
AccI GT'mk_AC
Cuts at: 0 1049 1197
Size: 1049 148
Acc65I G'GTAC_C
Cuts at: 0 730 1197
Size: 730 467
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1197
Size: 778 419
AflIII A'CryG_T
Cuts at: 0 341 875 1197
Size: 341 534 322
Fragments arranged by size:
534 341 322
ApoI r'AATT_y
Cuts at: 0 194 412 694 810 901 1197
Size: 194 218 282 116 91 296
Fragments arranged by size:
296 282 218 194 116 91
AseI AT'TA_AT
Cuts at: 0 1175 1197
Size: 1175 22
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 722 755 1197
Size: 722 33 442
Fragments arranged by size:
722 442 33
BamHI G'GATC_C
Cuts at: 0 87 1197
Size: 87 1110
BanI G'GyrC_C
Cuts at: 0 526 730 1197
Size: 526 204 467
Fragments arranged by size:
526 467 204
BanII G_rGCy'C
Cuts at: 0 767 1197
Size: 767 430
BbsI GAAGACnn'nnnn_
Cuts at: 0 237 1197
Size: 237 960
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1021 1055 1197
Size: 1021 34 142
Fragments arranged by size:
1021 142 34
BmgBI CAC'GTC
Cuts at: 0 342 1197
Size: 342 855
BmrI ACTGGGnnnn_n'
Cuts at: 0 138 291 936 1197
Size: 138 153 645 261
Fragments arranged by size:
645 261 153 138
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 989 1197
Size: 989 208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 906 1038 1197
Size: 86 820 132 159
Fragments arranged by size:
820 159 132 86
Bpu10I CC'TnA_GC
Cuts at: 0 563 1197
Size: 563 634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 1197
Size: 506 691
BsaI GGTCTCn'nnnn_
Cuts at: 0 380 1197
Size: 380 817
BsaHI Gr'CG_yC
Cuts at: 0 1055 1197
Size: 1055 142
BsaWI w'CCGG_w
Cuts at: 0 1107 1197
Size: 1107 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 834 864 1011 1041 1197
Size: 834 30 147 30 156
Fragments arranged by size:
834 156 147 30 30
BseYI C'CCAG_C
Cuts at: 0 726 1197
Size: 726 471
BsiHKAI G_wGCw'C
Cuts at: 0 767 1197
Size: 767 430
BsmI GAATG_Cn'
Cuts at: 0 51 1103 1197
Size: 51 1052 94
Fragments arranged by size:
1052 94 51
Bsp1286I G_dGCh'C
Cuts at: 0 767 1197
Size: 767 430
BspEI T'CCGG_A
Cuts at: 0 1107 1197
Size: 1107 90
BsrBI CCG'CTC
Cuts at: 0 1022 1197
Size: 1022 175
BstYI r'GATC_y
Cuts at: 0 87 557 1197
Size: 87 470 640
Fragments arranged by size:
640 470 87
BtsI GCAGTG_nn'
Cuts at: 0 942 1197
Size: 942 255
DraI TTT'AAA
Cuts at: 0 815 1197
Size: 815 382
EaeI y'GGCC_r
Cuts at: 0 489 638 1197
Size: 489 149 559
Fragments arranged by size:
559 489 149
EcoICRI GAG'CTC
Cuts at: 0 765 1197
Size: 765 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 778 906 1038 1197
Size: 86 692 128 132 159
Fragments arranged by size:
692 159 132 128 86
EcoO109I rG'GnC_Cy
Cuts at: 0 229 601 1137 1197
Size: 229 372 536 60
Fragments arranged by size:
536 372 229 60
EcoRI G'AATT_C
Cuts at: 0 194 412 1197
Size: 194 218 785
Fragments arranged by size:
785 218 194
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 715 1197
Size: 715 482
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1011 1043 1176 1197
Size: 1011 32 133 21
Fragments arranged by size:
1011 133 32 21
KpnI G_GTAC'C
Cuts at: 0 734 1197
Size: 734 463
MscI TGG'CCA
Cuts at: 0 491 640 1197
Size: 491 149 557
Fragments arranged by size:
557 491 149
MslI CAynn'nnrTG
Cuts at: 0 106 1000 1197
Size: 106 894 197
Fragments arranged by size:
894 197 106
NspI r_CATG'y
Cuts at: 0 879 999 1197
Size: 879 120 198
Fragments arranged by size:
879 198 120
PasI CC'CwG_GG
Cuts at: 0 597 1197
Size: 597 600
PciI A'CATG_T
Cuts at: 0 875 1197
Size: 875 322
PpuMI rG'GwC_Cy
Cuts at: 0 229 601 1197
Size: 229 372 596
Fragments arranged by size:
596 372 229
PshAI GACnn'nnGTC
Cuts at: 0 227 1012 1197
Size: 227 785 185
Fragments arranged by size:
785 227 185
PsiI TTA'TAA
Cuts at: 0 1163 1197
Size: 1163 34
PstI C_TGCA'G
Cuts at: 0 1014 1197
Size: 1014 183
SacI G_AGCT'C
Cuts at: 0 767 1197
Size: 767 430
SexAI A'CCwGG_T
Cuts at: 0 370 1197
Size: 370 827
SfcI C'TryA_G
Cuts at: 0 533 1010 1197
Size: 533 477 187
Fragments arranged by size:
533 477 187
SmlI C'TyrA_G
Cuts at: 0 521 1197
Size: 521 676
StyI C'CwwG_G
Cuts at: 0 1150 1197
Size: 1150 47
SwaI ATTT'AAAT
Cuts at: 0 815 1197
Size: 815 382
TatI w'GTAC_w
Cuts at: 0 625 1197
Size: 625 572
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 851 883 1197
Size: 851 32 314
Fragments arranged by size:
851 314 32
XbaI T'CTAG_A
Cuts at: 0 823 1197
Size: 823 374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 1197
Size: 306 891
XmnI GAAnn'nnTTC
Cuts at: 0 698 1197
Size: 698 499
ZraI GAC'GTC
Cuts at: 0 1056 1197
Size: 1056 141
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII ApoI AseI BaeI
BamHI BanI BanII BbsI BcgI BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseYI
BsiHKAI BsmI Bsp1286I BspEI BsrBI BstYI BtsI DraI
EaeI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I KpnI
MscI MslI NspI PasI PciI PpuMI PshAI PsiI
PstI SacI SexAI SfcI SmlI StyI SwaI TatI
TstI XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmtI BsaAI BsaBI BseRI BsgI BsiEI BsiWI
BsmBI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraIII DrdI EagI EarI EciI EcoNI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI MmeI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PfoI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI TaqII Tth111I XhoI XmaI