Restriction map for
pFN21AE4376
(Linear) (Six-base) MAPSORT of: KIEE4376.seq Check: 3887 from: 1 to: 1197
KIEE4376.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 445 514 583 652 721 1170 1197
Size: 376 69 69 69 69 69 449 27
Fragments arranged by size:
449 376 69 69 69 69 69 27
AlwNI CAG_nnn'CTG
Cuts at: 0 33 276 345 414 483 552 621 690
Size: 33 243 69 69 69 69 69 69
Cuts at: 690 1124 1197
Size: 434 73
Fragments arranged by size:
434 243 73 69 69 69 69 69
69 33
ApaI G_GGCC'C
Cuts at: 0 903 1197
Size: 903 294
ApoI r'AATT_y
Cuts at: 0 1108 1197
Size: 1108 89
BanI G'GyrC_C
Cuts at: 0 145 1197
Size: 145 1052
BanII G_rGCy'C
Cuts at: 0 124 308 903 1197
Size: 124 184 595 294
Fragments arranged by size:
595 294 184 124
BbeI G_GCGC'C
Cuts at: 0 149 1197
Size: 149 1048
BbsI GAAGACnn'nnnn_
Cuts at: 0 778 1103 1197
Size: 778 325 94
Fragments arranged by size:
778 325 94
BbvCI CC'TCA_GC
Cuts at: 0 926 1197
Size: 926 271
BglII A'GATC_T
Cuts at: 0 332 401 470 539 608 677 1197
Size: 332 69 69 69 69 69 520
Fragments arranged by size:
520 332 69 69 69 69 69
Bme1580I G_kGCm'C
Cuts at: 0 903 1197
Size: 903 294
Bpu10I CC'TnA_GC
Cuts at: 0 926 1197
Size: 926 271
BsaAI yAC'GTr
Cuts at: 0 1013 1197
Size: 1013 184
BsaHI Gr'CG_yC
Cuts at: 0 146 1197
Size: 146 1051
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 958 988 1197
Size: 958 30 209
Fragments arranged by size:
958 209 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 943 1197
Size: 154 789 254
Fragments arranged by size:
789 254 154
BseYI C'CCAG_C
Cuts at: 0 33 184 226 281 350 695 854 902
Size: 33 151 42 55 69 345 159 48
Cuts at: 902 1045 1197
Size: 143 152
Fragments arranged by size:
345 159 152 151 143 69 55 48
42 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 1197
Size: 46 1151
BsiHKAI G_wGCw'C
Cuts at: 0 124 308 1197
Size: 124 184 889
Fragments arranged by size:
889 184 124
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1184 1197
Size: 1184 13
Bsp1286I G_dGCh'C
Cuts at: 0 124 308 903 1197
Size: 124 184 595 294
Fragments arranged by size:
595 294 184 124
BsrDI GCAATG_nn'
Cuts at: 0 1024 1197
Size: 1024 173
BstYI r'GATC_y
Cuts at: 0 51 332 401 470 539 608 677 1160
Size: 51 281 69 69 69 69 69 483
Cuts at: 1160 1197
Size: 37
Fragments arranged by size:
483 281 69 69 69 69 69 51
37
BtgI C'CryG_G
Cuts at: 0 141 1197
Size: 141 1056
BtsI GCAGTG_nn'
Cuts at: 0 302 371 440 509 578 647 716 1125
Size: 302 69 69 69 69 69 69 409
Cuts at: 1125 1197
Size: 72
Fragments arranged by size:
409 302 72 69 69 69 69 69
69
ClaI AT'CG_AT
Cuts at: 0 1137 1197
Size: 1137 60
EaeI y'GGCC_r
Cuts at: 0 111 138 197 1197
Size: 111 27 59 1000
Fragments arranged by size:
1000 111 59 27
EcoICRI GAG'CTC
Cuts at: 0 122 306 1197
Size: 122 184 891
Fragments arranged by size:
891 184 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 445 514 583 652 721 1170 1197
Size: 376 69 69 69 69 69 449 27
Fragments arranged by size:
449 376 69 69 69 69 69 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 238 1000 1197
Size: 238 762 197
Fragments arranged by size:
762 238 197
EcoO109I rG'GnC_Cy
Cuts at: 0 189 222 899 1197
Size: 189 33 677 298
Fragments arranged by size:
677 298 189 33
HaeII r_GCGC'y
Cuts at: 0 149 1197
Size: 149 1048
HincII GTy'rAC
Cuts at: 0 1149 1197
Size: 1149 48
KasI G'GCGC_C
Cuts at: 0 145 1197
Size: 145 1052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1197
Size: 79 1118
MscI TGG'CCA
Cuts at: 0 113 140 199 1197
Size: 113 27 59 998
Fragments arranged by size:
998 113 59 27
MspA1I CmG'CkG
Cuts at: 0 33 354 699 858 948 1056 1197
Size: 33 321 345 159 90 108 141
Fragments arranged by size:
345 321 159 141 108 90 33
NarI GG'CG_CC
Cuts at: 0 146 1197
Size: 146 1051
NcoI C'CATG_G
Cuts at: 0 141 1197
Size: 141 1056
PflMI CCAn_nnn'nTGG
Cuts at: 0 812 1197
Size: 812 385
PfoI T'CCnGG_A
Cuts at: 0 41 958 976 1197
Size: 41 917 18 221
Fragments arranged by size:
917 221 41 18
PmlI CAC'GTG
Cuts at: 0 1013 1197
Size: 1013 184
PpuMI rG'GwC_Cy
Cuts at: 0 189 222 1197
Size: 189 33 975
Fragments arranged by size:
975 189 33
PspOMI G'GGCC_C
Cuts at: 0 899 1197
Size: 899 298
PstI C_TGCA'G
Cuts at: 0 297 329 366 398 435 467 504 536
Size: 297 32 37 32 37 32 37 32
Cuts at: 536 573 605 642 674 711 953 1197
Size: 37 32 37 32 37 242 244
Fragments arranged by size:
297 244 242 37 37 37 37 37
37 32 32 32 32 32 32
PvuII CAG'CTG
Cuts at: 0 33 354 699 948 1197
Size: 33 321 345 249 249
Fragments arranged by size:
345 321 249 249 33
SacI G_AGCT'C
Cuts at: 0 124 308 1197
Size: 124 184 889
Fragments arranged by size:
889 184 124
SfcI C'TryA_G
Cuts at: 0 125 293 325 362 394 431 463 500
Size: 125 168 32 37 32 37 32 37
Cuts at: 500 532 569 601 638 670 707 943 949
Size: 32 37 32 37 32 37 236 6
Cuts at: 949 1197
Size: 248
Fragments arranged by size:
248 236 168 125 37 37 37 37
37 37 32 32 32 32 32 32
6
SfoI GGC'GCC
Cuts at: 0 147 1197
Size: 147 1050
StyI C'CwwG_G
Cuts at: 0 11 19 141 218 806 825 1197
Size: 11 8 122 77 588 19 372
Fragments arranged by size:
588 372 122 77 19 11 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 289 358 427 496 565 634 703 778
Size: 289 69 69 69 69 69 69 75
Cuts at: 778 1011 1059 1197
Size: 233 48 138
Fragments arranged by size:
289 233 138 75 69 69 69 69
69 69 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 997 1029 1034 1066 1197
Size: 997 32 5 32 131
Fragments arranged by size:
997 131 32 32 5
Tth111I GACn'n_nGTC
Cuts at: 0 874 1197
Size: 874 323
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 106 1197
Size: 106 1091
Enzymes that do cut:
AcuI AlwNI ApaI ApoI BanI BanII BbeI BbsI
BbvCI BglII Bme1580I Bpu10I BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrDI BstYI BtgI
BtsI ClaI EaeI EcoICRI Eco57MI EcoNI EcoO109I HaeII
HincII KasI MmeI MscI MspA1I NarI NcoI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SfcI
SfoI StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaBI BsaWI BsiEI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DraI
DraIII DrdI EagI EarI EciI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MslI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI XbaI XhoI XmaI XmnI ZraI