Restriction map for
pFN21AE4375
(Linear) (Six-base) MAPSORT of: KIEE4375.seq Check: 2970 from: 1 to: 1185
KIEE4375.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 737 1185
Size: 737 448
AccI GT'mk_AC
Cuts at: 0 722 1185
Size: 722 463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 1185
Size: 685 500
AgeI A'CCGG_T
Cuts at: 0 9 1185
Size: 9 1176
AleI CACnn'nnGTG
Cuts at: 0 865 1185
Size: 865 320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 211 945 1185
Size: 211 734 240
Fragments arranged by size:
734 240 211
ApaI G_GGCC'C
Cuts at: 0 663 1185
Size: 663 522
BanI G'GyrC_C
Cuts at: 0 690 1185
Size: 690 495
BanII G_rGCy'C
Cuts at: 0 663 828 929 1185
Size: 663 165 101 256
Fragments arranged by size:
663 256 165 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 42 638 719 1024 1123 1185
Size: 42 596 81 305 99 62
Fragments arranged by size:
596 305 99 81 62 42
BglI GCCn_nnn'nGGC
Cuts at: 0 160 490 741 1185
Size: 160 330 251 444
Fragments arranged by size:
444 330 251 160
BglII A'GATC_T
Cuts at: 0 95 1185
Size: 95 1090
BlpI GC'TnA_GC
Cuts at: 0 876 1185
Size: 876 309
Bme1580I G_kGCm'C
Cuts at: 0 663 1185
Size: 663 522
BmrI ACTGGGnnnn_n'
Cuts at: 0 348 731 1185
Size: 348 383 454
Fragments arranged by size:
454 383 348
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 231 1185
Size: 231 954
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 1185
Size: 664 521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1185
Size: 121 1064
BsaBI GATnn'nnATC
Cuts at: 0 131 1185
Size: 131 1054
BsaHI Gr'CG_yC
Cuts at: 0 1073 1185
Size: 1073 112
BsaWI w'CCGG_w
Cuts at: 0 9 1185
Size: 9 1176
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 454 505 661 1185
Size: 394 60 51 156 524
Fragments arranged by size:
524 394 156 60 51
BseYI C'CCAG_C
Cuts at: 0 818 896 1185
Size: 818 78 289
Fragments arranged by size:
818 289 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1185
Size: 640 545
BsiEI CG_ry'CG
Cuts at: 0 1069 1185
Size: 1069 116
Bsp1286I G_dGCh'C
Cuts at: 0 663 828 929 1185
Size: 663 165 101 256
Fragments arranged by size:
663 256 165 101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 737 1185
Size: 737 448
BsrBI CCG'CTC
Cuts at: 0 875 1185
Size: 875 310
BsrFI r'CCGG_y
Cuts at: 0 9 184 693 1185
Size: 9 175 509 492
Fragments arranged by size:
509 492 175 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 705 912 969 1185
Size: 705 207 57 216
Fragments arranged by size:
705 216 207 57
BstYI r'GATC_y
Cuts at: 0 95 1185
Size: 95 1090
Bsu36I CC'TnA_GG
Cuts at: 0 499 1185
Size: 499 686
BtgI C'CryG_G
Cuts at: 0 224 686 1156 1185
Size: 224 462 470 29
Fragments arranged by size:
470 462 224 29
BtsI GCAGTG_nn'
Cuts at: 0 125 1185
Size: 125 1060
EaeI y'GGCC_r
Cuts at: 0 221 695 786 902 971 1066 1131 1185
Size: 221 474 91 116 69 95 65 54
Fragments arranged by size:
474 221 116 95 91 69 65 54
EagI C'GGCC_G
Cuts at: 0 1066 1185
Size: 1066 119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 685 1185
Size: 664 21 500
Fragments arranged by size:
664 500 21
EcoO109I rG'GnC_Cy
Cuts at: 0 659 660 760 839 1185
Size: 659 1 100 79 346
Fragments arranged by size:
659 346 100 79 1
FspI TGC'GCA
Cuts at: 0 595 655 1185
Size: 595 60 530
Fragments arranged by size:
595 530 60
FspAI rTGC'GCAy
Cuts at: 0 595 1185
Size: 595 590
HaeII r_GCGC'y
Cuts at: 0 507 1185
Size: 507 678
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 322 354 929 961 967 999 1185
Size: 322 32 575 32 6 32 186
Fragments arranged by size:
575 322 186 32 32 32 6
HindIII A'AGCT_T
Cuts at: 0 1174 1185
Size: 1174 11
MscI TGG'CCA
Cuts at: 0 223 904 1185
Size: 223 681 281
Fragments arranged by size:
681 281 223
MslI CAynn'nnrTG
Cuts at: 0 217 589 616 833 865 967 1185
Size: 217 372 27 217 32 102 218
Fragments arranged by size:
372 218 217 217 102 32 27
MspA1I CmG'CkG
Cuts at: 0 240 896 981 1185
Size: 240 656 85 204
Fragments arranged by size:
656 240 204 85
NaeI GCC'GGC
Cuts at: 0 695 1185
Size: 695 490
NcoI C'CATG_G
Cuts at: 0 224 1156 1185
Size: 224 932 29
Fragments arranged by size:
932 224 29
NgoMIV G'CCGG_C
Cuts at: 0 693 1185
Size: 693 492
NspI r_CATG'y
Cuts at: 0 450 567 1127 1185
Size: 450 117 560 58
Fragments arranged by size:
560 450 117 58
PasI CC'CwG_GG
Cuts at: 0 765 1185
Size: 765 420
PflMI CCAn_nnn'nTGG
Cuts at: 0 243 1185
Size: 243 942
PpuMI rG'GwC_Cy
Cuts at: 0 760 1185
Size: 760 425
PspOMI G'GGCC_C
Cuts at: 0 659 1185
Size: 659 526
PstI C_TGCA'G
Cuts at: 0 734 1185
Size: 734 451
SfcI C'TryA_G
Cuts at: 0 730 1185
Size: 730 455
SmlI C'TyrA_G
Cuts at: 0 136 1185
Size: 136 1049
SphI G_CATG'C
Cuts at: 0 450 1127 1185
Size: 450 677 58
Fragments arranged by size:
677 450 58
StyI C'CwwG_G
Cuts at: 0 155 224 287 863 1156 1185
Size: 155 69 63 576 293 29
Fragments arranged by size:
576 293 155 69 63 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 888 1185
Size: 888 297
TatI w'GTAC_w
Cuts at: 0 560 1185
Size: 560 625
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7 39 1185
Size: 7 32 1146
Fragments arranged by size:
1146 32 7
Tth111I GACn'n_nGTC
Cuts at: 0 718 1185
Size: 718 467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 156 1185
Size: 156 1029
Enzymes that do cut:
AarI AccI AcuI AgeI AleI AlfI ApaI BanI
BanII BbsI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI Bsp1286I BspMI BsrBI BsrFI BstXI BstYI Bsu36I
BtgI BtsI EaeI EagI Eco57MI EcoO109I FspI FspAI
HaeII Hin4I HindIII MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PflMI PpuMI PspOMI PstI SfcI
SmlI SphI StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AloI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BmgBI BmtI Bpu10I BsaI BsaAI BsaXI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI HincII HpaI KasI KpnI MfeI MluI
MmeI NarI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI XbaI
XhoI XmaI XmnI ZraI