Restriction map for
pFN21AE4374
(Linear) (Six-base) MAPSORT of: KIEE4374.seq Check: 6317 from: 1 to: 1176
KIEE4374.orf
With 182 enzymes: SgfI *
March 22, 2012 17:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 330 1176
Size: 330 846
AccI GT'mk_AC
Cuts at: 0 815 900 1176
Size: 815 85 276
Fragments arranged by size:
815 276 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1147 1176
Size: 1147 29
AleI CACnn'nnGTG
Cuts at: 0 790 1176
Size: 790 386
AlwNI CAG_nnn'CTG
Cuts at: 0 969 1176
Size: 969 207
ApaI G_GGCC'C
Cuts at: 0 707 773 978 1176
Size: 707 66 205 198
Fragments arranged by size:
707 205 198 66
ApaLI G'TGCA_C
Cuts at: 0 1039 1176
Size: 1039 137
AvaI C'yCGr_G
Cuts at: 0 1011 1176
Size: 1011 165
BanI G'GyrC_C
Cuts at: 0 490 592 1015 1176
Size: 490 102 423 161
Fragments arranged by size:
490 423 161 102
BanII G_rGCy'C
Cuts at: 0 161 533 707 773 978 1155 1176
Size: 161 372 174 66 205 177 21
Fragments arranged by size:
372 205 177 174 161 66 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 181 1176
Size: 181 995
BglII A'GATC_T
Cuts at: 0 23 1176
Size: 23 1153
BlpI GC'TnA_GC
Cuts at: 0 387 1176
Size: 387 789
Bme1580I G_kGCm'C
Cuts at: 0 33 707 773 978 1018 1043 1176
Size: 33 674 66 205 40 25 133
Fragments arranged by size:
674 205 133 66 40 33 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 480 580 1030 1176
Size: 480 100 450 146
Fragments arranged by size:
480 450 146 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 261 445 454 626 862 902 1000
Size: 72 189 184 9 172 236 40 98
Cuts at: 1000 1176
Size: 176
Fragments arranged by size:
236 189 184 176 172 98 72 40
9
Bpu10I CC'TnA_GC
Cuts at: 0 1097 1176
Size: 1097 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1176
Size: 718 458
BsaI GGTCTCn'nnnn_
Cuts at: 0 630 1176
Size: 630 546
BsaHI Gr'CG_yC
Cuts at: 0 853 1176
Size: 853 323
BsaWI w'CCGG_w
Cuts at: 0 374 1176
Size: 374 802
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 256 286 599 629 1123 1153 1176
Size: 256 30 313 30 494 30 23
Fragments arranged by size:
494 313 256 30 30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 264 997 1176
Size: 75 189 733 179
Fragments arranged by size:
733 189 179 75
BseYI C'CCAG_C
Cuts at: 0 221 267 537 642 1176
Size: 221 46 270 105 534
Fragments arranged by size:
534 270 221 105 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 1176
Size: 247 929
BsiHKAI G_wGCw'C
Cuts at: 0 518 1043 1176
Size: 518 525 133
Fragments arranged by size:
525 518 133
Bsp1286I G_dGCh'C
Cuts at: 0 33 161 518 533 707 773 978 1018
Size: 33 128 357 15 174 66 205 40
Cuts at: 1018 1043 1155 1176
Size: 25 112 21
Fragments arranged by size:
357 205 174 128 112 66 40 33
25 21 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 330 1176
Size: 116 214 846
Fragments arranged by size:
846 214 116
BsrBI CCG'CTC
Cuts at: 0 13 1176
Size: 13 1163
BsrFI r'CCGG_y
Cuts at: 0 446 487 1176
Size: 446 41 689
Fragments arranged by size:
689 446 41
BsrGI T'GTAC_A
Cuts at: 0 1052 1176
Size: 1052 124
BssHII G'CGCG_C
Cuts at: 0 558 560 1176
Size: 558 2 616
Fragments arranged by size:
616 558 2
BssSI C'ACGA_G
Cuts at: 0 1156 1176
Size: 1156 20
BstAPI GCAn_nnn'nTGC
Cuts at: 0 969 1022 1176
Size: 969 53 154
Fragments arranged by size:
969 154 53
BstXI CCAn_nnnn'nTGG
Cuts at: 0 832 1176
Size: 832 344
BstYI r'GATC_y
Cuts at: 0 23 1133 1176
Size: 23 1110 43
Fragments arranged by size:
1110 43 23
BtgI C'CryG_G
Cuts at: 0 1082 1176
Size: 1082 94
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 672 707 1176
Size: 672 35 469
Fragments arranged by size:
672 469 35
EaeI y'GGCC_r
Cuts at: 0 2 635 1176
Size: 2 633 541
Fragments arranged by size:
633 541 2
EarI CTCTTCn'nnn_
Cuts at: 0 355 1176
Size: 355 821
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 343 433 1176
Size: 343 90 743
Fragments arranged by size:
743 343 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 261 445 454 626 862 902 1000
Size: 72 189 184 9 172 236 40 98
Cuts at: 1000 1147 1176
Size: 147 29
Fragments arranged by size:
236 189 184 172 147 98 72 40
29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 982 1176
Size: 982 194
EcoO109I rG'GnC_Cy
Cuts at: 0 474 753 975 1176
Size: 474 279 222 201
Fragments arranged by size:
474 279 222 201
HindIII A'AGCT_T
Cuts at: 0 37 1176
Size: 37 1139
MslI CAynn'nnrTG
Cuts at: 0 196 457 790 830 1176
Size: 196 261 333 40 346
Fragments arranged by size:
346 333 261 196 40
MspA1I CmG'CkG
Cuts at: 0 114 300 861 1176
Size: 114 186 561 315
Fragments arranged by size:
561 315 186 114
NaeI GCC'GGC
Cuts at: 0 448 489 1176
Size: 448 41 687
Fragments arranged by size:
687 448 41
NcoI C'CATG_G
Cuts at: 0 1082 1176
Size: 1082 94
NgoMIV G'CCGG_C
Cuts at: 0 446 487 1176
Size: 446 41 689
Fragments arranged by size:
689 446 41
NspI r_CATG'y
Cuts at: 0 195 1176
Size: 195 981
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 740 772 1150 1176
Size: 517 32 191 32 378 26
Fragments arranged by size:
517 378 191 32 32 26
PpuMI rG'GwC_Cy
Cuts at: 0 474 753 1176
Size: 474 279 423
Fragments arranged by size:
474 423 279
PshAI GACnn'nnGTC
Cuts at: 0 622 1176
Size: 622 554
PspOMI G'GGCC_C
Cuts at: 0 703 769 974 1176
Size: 703 66 205 202
Fragments arranged by size:
703 205 202 66
PstI C_TGCA'G
Cuts at: 0 105 113 299 1176
Size: 105 8 186 877
Fragments arranged by size:
877 186 105 8
PvuII CAG'CTG
Cuts at: 0 114 300 1176
Size: 114 186 876
Fragments arranged by size:
876 186 114
SanDI GG'GwC_CC
Cuts at: 0 474 1176
Size: 474 702
SfcI C'TryA_G
Cuts at: 0 101 109 295 1176
Size: 101 8 186 881
Fragments arranged by size:
881 186 101 8
SmlI C'TyrA_G
Cuts at: 0 733 1176
Size: 733 443
StyI C'CwwG_G
Cuts at: 0 52 1082 1176
Size: 52 1030 94
Fragments arranged by size:
1030 94 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 281 529 580 651 1176
Size: 281 248 51 71 525
Fragments arranged by size:
525 281 248 71 51
TatI w'GTAC_w
Cuts at: 0 1052 1176
Size: 1052 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 256 288 599 631 1176
Size: 256 32 311 32 545
Fragments arranged by size:
545 311 256 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 472 896 1176
Size: 472 424 280
Fragments arranged by size:
472 424 280
XmnI GAAnn'nnTTC
Cuts at: 0 319 1176
Size: 319 857
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstXI BstYI BtgI CspCI EaeI EarI
EciI Eco57MI EcoNI EcoO109I HindIII MslI MspA1I NaeI
NcoI NgoMIV NspI PpiI PpuMI PshAI PspOMI PstI
PvuII SanDI SfcI SmlI StyI TaqII TatI TstI
Tth111I XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BmgBI BmtI BplI BsaAI BsaBI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI DraI DraIII DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XcmI XhoI
XmaI ZraI