Restriction map for
pFN21AE4370
(Linear) (Six-base) MAPSORT of: KIEE4370.seq Check: 2179 from: 1 to: 1161
KIEE4370.orf
With 182 enzymes: SgfI *
March 22, 2012 17:50 ..
AccI GT'mk_AC
Cuts at: 0 205 1161
Size: 205 956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 48 1161
Size: 48 1113
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 369 401 1161
Size: 369 32 760
Fragments arranged by size:
760 369 32
AlwNI CAG_nnn'CTG
Cuts at: 0 361 1161
Size: 361 800
ApaI G_GGCC'C
Cuts at: 0 672 1161
Size: 672 489
ApoI r'AATT_y
Cuts at: 0 912 1161
Size: 912 249
BanI G'GyrC_C
Cuts at: 0 811 1161
Size: 811 350
BanII G_rGCy'C
Cuts at: 0 91 672 1000 1128 1161
Size: 91 581 328 128 33
Fragments arranged by size:
581 328 128 91 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 884 1161
Size: 884 277
BbvCI CC'TCA_GC
Cuts at: 0 362 866 1161
Size: 362 504 295
Fragments arranged by size:
504 362 295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 362 396 408 442 1161
Size: 362 34 12 34 719
Fragments arranged by size:
719 362 34 34 12
BclI T'GATC_A
Cuts at: 0 491 926 1161
Size: 491 435 235
Fragments arranged by size:
491 435 235
BfrBI ATG'CAT
Cuts at: 0 563 1161
Size: 563 598
BglI GCCn_nnn'nGGC
Cuts at: 0 125 1161
Size: 125 1036
Bme1580I G_kGCm'C
Cuts at: 0 672 1161
Size: 672 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 806 856 1161
Size: 763 43 50 305
Fragments arranged by size:
763 305 50 43
Bpu10I CC'TnA_GC
Cuts at: 0 90 362 866 1161
Size: 90 272 504 295
Fragments arranged by size:
504 295 272 90
BsaWI w'CCGG_w
Cuts at: 0 957 1161
Size: 957 204
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 369 399 1161
Size: 369 30 762
Fragments arranged by size:
762 369 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 677 1161
Size: 677 484
BseYI C'CCAG_C
Cuts at: 0 312 502 1161
Size: 312 190 659
Fragments arranged by size:
659 312 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 448 1161
Size: 155 293 713
Fragments arranged by size:
713 293 155
BsiHKAI G_wGCw'C
Cuts at: 0 7 91 404 1128 1161
Size: 7 84 313 724 33
Fragments arranged by size:
724 313 84 33 7
BsmI GAATG_Cn'
Cuts at: 0 1139 1161
Size: 1139 22
Bsp1286I G_dGCh'C
Cuts at: 0 7 91 404 672 1000 1128 1161
Size: 7 84 313 268 328 128 33
Fragments arranged by size:
328 313 268 128 84 33 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 388 1161
Size: 388 773
BsrDI GCAATG_nn'
Cuts at: 0 467 1161
Size: 467 694
BstYI r'GATC_y
Cuts at: 0 29 1040 1161
Size: 29 1011 121
Fragments arranged by size:
1011 121 29
BstZ17I GTA'TAC
Cuts at: 0 206 1161
Size: 206 955
EaeI y'GGCC_r
Cuts at: 0 219 282 566 885 1101 1161
Size: 219 63 284 319 216 60
Fragments arranged by size:
319 284 219 216 63 60
EarI CTCTTCn'nnn_
Cuts at: 0 79 184 620 988 1161
Size: 79 105 436 368 173
Fragments arranged by size:
436 368 173 105 79
EcoICRI GAG'CTC
Cuts at: 0 89 1126 1161
Size: 89 1037 35
Fragments arranged by size:
1037 89 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 806 856 1161
Size: 763 43 50 305
Fragments arranged by size:
763 305 50 43
EcoO109I rG'GnC_Cy
Cuts at: 0 668 669 1161
Size: 668 1 492
Fragments arranged by size:
668 492 1
HaeII r_GCGC'y
Cuts at: 0 1031 1161
Size: 1031 130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 804 1161
Size: 544 260 357
Fragments arranged by size:
544 357 260
MscI TGG'CCA
Cuts at: 0 221 284 1103 1161
Size: 221 63 819 58
Fragments arranged by size:
819 221 63 58
MslI CAynn'nnrTG
Cuts at: 0 568 1161
Size: 568 593
MspA1I CmG'CkG
Cuts at: 0 99 865 909 1161
Size: 99 766 44 252
Fragments arranged by size:
766 252 99 44
NsiI A_TGCA'T
Cuts at: 0 565 1161
Size: 565 596
NspI r_CATG'y
Cuts at: 0 982 1161
Size: 982 179
PflMI CCAn_nnn'nTGG
Cuts at: 0 526 1161
Size: 526 635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 369 401 1161
Size: 369 32 760
Fragments arranged by size:
760 369 32
PspOMI G'GGCC_C
Cuts at: 0 668 1161
Size: 668 493
PstI C_TGCA'G
Cuts at: 0 470 1161
Size: 470 691
PvuII CAG'CTG
Cuts at: 0 99 1161
Size: 99 1062
SacI G_AGCT'C
Cuts at: 0 91 1128 1161
Size: 91 1037 33
Fragments arranged by size:
1037 91 33
SapI GCTCTTCn'nnn_
Cuts at: 0 79 988 1161
Size: 79 909 173
Fragments arranged by size:
909 173 79
ScaI AGT'ACT
Cuts at: 0 53 1161
Size: 53 1108
SfcI C'TryA_G
Cuts at: 0 466 1161
Size: 466 695
SphI G_CATG'C
Cuts at: 0 982 1161
Size: 982 179
StuI AGG'CCT
Cuts at: 0 128 1161
Size: 128 1033
TatI w'GTAC_w
Cuts at: 0 51 1161
Size: 51 1110
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 636 1161
Size: 636 525
Enzymes that do cut:
AccI AlfI AloI AlwNI ApaI ApoI BanI BanII
BbsI BbvCI BcgI BclI BfrBI BglI Bme1580I BpmI
Bpu10I BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BstYI BstZ17I EaeI EarI EcoICRI
Eco57MI EcoO109I HaeII MmeI MscI MslI MspA1I NsiI
NspI PflMI PpiI PspOMI PstI PvuII SacI SapI
ScaI SfcI SphI StuI TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BciVI BglII BlpI BmgBI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StyI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI