Restriction map for
pFN21AE4369
(Linear) (Six-base) MAPSORT of: KIEE4369.seq Check: 6036 from: 1 to: 1158
KIEE4369.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AccI GT'mk_AC
Cuts at: 0 626 1158
Size: 626 532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 1158
Size: 467 691
AleI CACnn'nnGTG
Cuts at: 0 874 1158
Size: 874 284
AlwNI CAG_nnn'CTG
Cuts at: 0 392 479 1158
Size: 392 87 679
Fragments arranged by size:
679 392 87
ApaLI G'TGCA_C
Cuts at: 0 427 1158
Size: 427 731
ApoI r'AATT_y
Cuts at: 0 271 526 611 743 1158
Size: 271 255 85 132 415
Fragments arranged by size:
415 271 255 132 85
AvaI C'yCGr_G
Cuts at: 0 674 1158
Size: 674 484
BbsI GAAGACnn'nnnn_
Cuts at: 0 1151 1158
Size: 1151 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 1028 1158
Size: 1028 130
BlpI GC'TnA_GC
Cuts at: 0 1070 1158
Size: 1070 88
Bme1580I G_kGCm'C
Cuts at: 0 431 1158
Size: 431 727
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 839 1158
Size: 839 319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1078 1158
Size: 1078 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1079 1158
Size: 520 559 79
Fragments arranged by size:
559 520 79
BsaI GGTCTCn'nnnn_
Cuts at: 0 128 1158
Size: 128 1030
BsaWI w'CCGG_w
Cuts at: 0 329 1158
Size: 329 829
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2 32 91 121 1158
Size: 2 30 59 30 1037
Fragments arranged by size:
1037 59 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 1158
Size: 7 1151
BseYI C'CCAG_C
Cuts at: 0 212 216 1045 1158
Size: 212 4 829 113
Fragments arranged by size:
829 212 113 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1158
Size: 526 632
BsiHKAI G_wGCw'C
Cuts at: 0 431 1158
Size: 431 727
BsmBI CGTCTCn'nnnn_
Cuts at: 0 994 1158
Size: 994 164
Bsp1286I G_dGCh'C
Cuts at: 0 431 1158
Size: 431 727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 473 1158
Size: 473 685
BssSI C'ACGA_G
Cuts at: 0 1009 1158
Size: 1009 149
BstBI TT'CG_AA
Cuts at: 0 312 1158
Size: 312 846
BtgI C'CryG_G
Cuts at: 0 1106 1158
Size: 1106 52
DraIII CAC_nnn'GTG
Cuts at: 0 1112 1158
Size: 1112 46
EaeI y'GGCC_r
Cuts at: 0 23 187 201 594 1158
Size: 23 164 14 393 564
Fragments arranged by size:
564 393 164 23 14
EarI CTCTTCn'nnn_
Cuts at: 0 529 808 1158
Size: 529 279 350
Fragments arranged by size:
529 350 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 1078 1158
Size: 467 611 80
Fragments arranged by size:
611 467 80
EcoO109I rG'GnC_Cy
Cuts at: 0 600 1158
Size: 600 558
EcoRI G'AATT_C
Cuts at: 0 526 743 1158
Size: 526 217 415
Fragments arranged by size:
526 415 217
FspI TGC'GCA
Cuts at: 0 863 1158
Size: 863 295
HaeII r_GCGC'y
Cuts at: 0 898 1158
Size: 898 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 975 1007 1158
Size: 975 32 151
Fragments arranged by size:
975 151 32
HincII GTy'rAC
Cuts at: 0 327 1158
Size: 327 831
MscI TGG'CCA
Cuts at: 0 25 189 203 596 1158
Size: 25 164 14 393 562
Fragments arranged by size:
562 393 164 25 14
MslI CAynn'nnrTG
Cuts at: 0 424 874 1158
Size: 424 450 284
Fragments arranged by size:
450 424 284
MspA1I CmG'CkG
Cuts at: 0 216 1049 1158
Size: 216 833 109
Fragments arranged by size:
833 216 109
PpuMI rG'GwC_Cy
Cuts at: 0 600 1158
Size: 600 558
PshAI GACnn'nnGTC
Cuts at: 0 140 1158
Size: 140 1018
PsiI TTA'TAA
Cuts at: 0 551 1158
Size: 551 607
PstI C_TGCA'G
Cuts at: 0 1042 1158
Size: 1042 116
PvuII CAG'CTG
Cuts at: 0 216 1049 1158
Size: 216 833 109
Fragments arranged by size:
833 216 109
ScaI AGT'ACT
Cuts at: 0 973 1158
Size: 973 185
SfcI C'TryA_G
Cuts at: 0 886 1038 1158
Size: 886 152 120
Fragments arranged by size:
886 152 120
SmaI CCC'GGG
Cuts at: 0 676 1158
Size: 676 482
SmlI C'TyrA_G
Cuts at: 0 499 1094 1158
Size: 499 595 64
Fragments arranged by size:
595 499 64
StyI C'CwwG_G
Cuts at: 0 45 95 569 774 1158
Size: 45 50 474 205 384
Fragments arranged by size:
474 384 205 50 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 95 152 226 1158
Size: 95 57 74 932
Fragments arranged by size:
932 95 74 57
TatI w'GTAC_w
Cuts at: 0 87 953 971 986 1150 1158
Size: 87 866 18 15 164 8
Fragments arranged by size:
866 164 87 18 15 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2 34 1158
Size: 2 32 1124
Fragments arranged by size:
1124 32 2
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 604 1053 1158
Size: 604 449 105
Fragments arranged by size:
604 449 105
XmaI C'CCGG_G
Cuts at: 0 674 1158
Size: 674 484
XmnI GAAnn'nnTTC
Cuts at: 0 579 1158
Size: 579 579
Enzymes that do cut:
AccI AcuI AleI AlwNI ApaLI ApoI AvaI BbsI
BciVI BlpI Bme1580I BplI BpmI BpuEI BsaI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BssSI BstBI BtgI DraIII EaeI EarI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII MscI MslI MspA1I
PpuMI PshAI PsiI PstI PvuII ScaI SfcI SmaI
SmlI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanI BanII BbeI BbvCI BcgI BclI
BfrBI BglI BglII BmgBI BmrI BmtI Bpu10I BsaAI
BsaBI BsaHI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI
EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspAI
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI ZraI