Restriction map for
pFN21AE4365
(Linear) (Six-base) MAPSORT of: KIEE4365.seq Check: 6822 from: 1 to: 1149
KIEE4365.orf
With 182 enzymes: SgfI *
March 22, 2012 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 119 436 673 1149
Size: 119 317 237 476
Fragments arranged by size:
476 317 237 119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1036 1104 1149
Size: 874 162 68 45
Fragments arranged by size:
874 162 68 45
AleI CACnn'nnGTG
Cuts at: 0 430 682 1149
Size: 430 252 467
Fragments arranged by size:
467 430 252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 685 1149
Size: 685 464
ApaI G_GGCC'C
Cuts at: 0 389 1149
Size: 389 760
ApaLI G'TGCA_C
Cuts at: 0 814 1149
Size: 814 335
AvaI C'yCGr_G
Cuts at: 0 211 349 357 709 1096 1149
Size: 211 138 8 352 387 53
Fragments arranged by size:
387 352 211 138 53 8
BanI G'GyrC_C
Cuts at: 0 131 424 584 661 1149
Size: 131 293 160 77 488
Fragments arranged by size:
488 293 160 131 77
BanII G_rGCy'C
Cuts at: 0 251 389 754 875 1149
Size: 251 138 365 121 274
Fragments arranged by size:
365 274 251 138 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 966 1149
Size: 966 183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 543 577 1149
Size: 543 34 572
Fragments arranged by size:
572 543 34
BclI T'GATC_A
Cuts at: 0 395 1149
Size: 395 754
BglI GCCn_nnn'nGGC
Cuts at: 0 140 144 479 593 1149
Size: 140 4 335 114 556
Fragments arranged by size:
556 335 140 114 4
BlpI GC'TnA_GC
Cuts at: 0 748 1149
Size: 748 401
Bme1580I G_kGCm'C
Cuts at: 0 348 389 475 589 664 818 961 1149
Size: 348 41 86 114 75 154 143 188
Fragments arranged by size:
348 188 154 143 114 86 75 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 727 985 1149
Size: 727 258 164
Fragments arranged by size:
727 258 164
BsaHI Gr'CG_yC
Cuts at: 0 548 1149
Size: 548 601
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 267 883 1149
Size: 267 616 266
Fragments arranged by size:
616 267 266
BseYI C'CCAG_C
Cuts at: 0 236 588 755 954 1149
Size: 236 352 167 199 195
Fragments arranged by size:
352 236 199 195 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 537 850 1149
Size: 504 33 313 299
Fragments arranged by size:
504 313 299 33
BsiEI CG_ry'CG
Cuts at: 0 11 1149
Size: 11 1138
BsiHKAI G_wGCw'C
Cuts at: 0 251 818 845 875 938 1149
Size: 251 567 27 30 63 211
Fragments arranged by size:
567 251 211 63 30 27
BsmI GAATG_Cn'
Cuts at: 0 79 340 1149
Size: 79 261 809
Fragments arranged by size:
809 261 79
Bsp1286I G_dGCh'C
Cuts at: 0 251 348 389 475 589 664 754 818
Size: 251 97 41 86 114 75 90 64
Cuts at: 818 845 875 938 961 1149
Size: 27 30 63 23 188
Fragments arranged by size:
251 188 114 97 90 86 75 64
63 41 30 27 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 119 313 436 619 673 750 1016 1052
Size: 119 194 123 183 54 77 266 36
Cuts at: 1052 1149
Size: 97
Fragments arranged by size:
266 194 183 123 119 97 77 54
36
BsrFI r'CCGG_y
Cuts at: 0 581 592 825 1132 1149
Size: 581 11 233 307 17
Fragments arranged by size:
581 307 233 17 11
BssHII G'CGCG_C
Cuts at: 0 741 743 1149
Size: 741 2 406
Fragments arranged by size:
741 406 2
BssSI C'ACGA_G
Cuts at: 0 817 1149
Size: 817 332
BstAPI GCAn_nnn'nTGC
Cuts at: 0 497 647 822 1149
Size: 497 150 175 327
Fragments arranged by size:
497 327 175 150
BstEII G'GTnAC_C
Cuts at: 0 624 762 1149
Size: 624 138 387
Fragments arranged by size:
624 387 138
BstXI CCAn_nnnn'nTGG
Cuts at: 0 948 1149
Size: 948 201
BtsI GCAGTG_nn'
Cuts at: 0 223 1149
Size: 223 926
DrdI GACnn_nn'nnGTC
Cuts at: 0 999 1078 1149
Size: 999 79 71
Fragments arranged by size:
999 79 71
EaeI y'GGCC_r
Cuts at: 0 51 509 1076 1149
Size: 51 458 567 73
Fragments arranged by size:
567 458 73 51
EcoICRI GAG'CTC
Cuts at: 0 249 873 1149
Size: 249 624 276
Fragments arranged by size:
624 276 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1036 1104 1149
Size: 874 162 68 45
Fragments arranged by size:
874 162 68 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 898 1149
Size: 898 251
EcoO109I rG'GnC_Cy
Cuts at: 0 151 302 386 1071 1149
Size: 151 151 84 685 78
Fragments arranged by size:
685 151 151 84 78
HincII GTy'rAC
Cuts at: 0 1083 1149
Size: 1083 66
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1028 1149
Size: 1028 121
MscI TGG'CCA
Cuts at: 0 511 1149
Size: 511 638
MslI CAynn'nnrTG
Cuts at: 0 430 682 1149
Size: 430 252 467
Fragments arranged by size:
467 430 252
MspA1I CmG'CkG
Cuts at: 0 518 578 1064 1149
Size: 518 60 486 85
Fragments arranged by size:
518 486 85 60
NaeI GCC'GGC
Cuts at: 0 594 1134 1149
Size: 594 540 15
Fragments arranged by size:
594 540 15
NgoMIV G'CCGG_C
Cuts at: 0 592 1132 1149
Size: 592 540 17
Fragments arranged by size:
592 540 17
NspI r_CATG'y
Cuts at: 0 1026 1149
Size: 1026 123
PasI CC'CwG_GG
Cuts at: 0 808 1149
Size: 808 341
PpuMI rG'GwC_Cy
Cuts at: 0 151 302 1071 1149
Size: 151 151 769 78
Fragments arranged by size:
769 151 151 78
PspOMI G'GGCC_C
Cuts at: 0 385 1149
Size: 385 764
PstI C_TGCA'G
Cuts at: 0 616 674 1149
Size: 616 58 475
Fragments arranged by size:
616 475 58
PvuII CAG'CTG
Cuts at: 0 518 1149
Size: 518 631
SacI G_AGCT'C
Cuts at: 0 251 875 1149
Size: 251 624 274
Fragments arranged by size:
624 274 251
SanDI GG'GwC_CC
Cuts at: 0 151 1149
Size: 151 998
SfcI C'TryA_G
Cuts at: 0 612 670 1149
Size: 612 58 479
Fragments arranged by size:
612 479 58
SmaI CCC'GGG
Cuts at: 0 213 351 1149
Size: 213 138 798
Fragments arranged by size:
798 213 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 25 694 987 1149
Size: 25 669 293 162
Fragments arranged by size:
669 293 162 25
TatI w'GTAC_w
Cuts at: 0 1055 1149
Size: 1055 94
Tth111I GACn'n_nGTC
Cuts at: 0 547 1149
Size: 547 602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 61 1149
Size: 61 1088
XmaI C'CCGG_G
Cuts at: 0 211 349 1149
Size: 211 138 800
Fragments arranged by size:
800 211 138
Enzymes that do cut:
AarI AcuI AleI AlfI ApaI ApaLI AvaI BanI
BanII BbsI BcgI BclI BglI BlpI Bme1580I BsaI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrFI BssHII BssSI BstAPI BstEII BstXI BtsI
DrdI EaeI EcoICRI Eco57MI EcoNI EcoO109I HincII MmeI
MscI MslI MspA1I NaeI NgoMIV NspI PasI PpuMI
PspOMI PstI PvuII SacI SanDI SfcI SmaI TaqII
TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BfrBI BglII BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaWI BsaXI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII EagI EarI EciI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HindIII HpaI
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TstI XbaI XhoI XmnI ZraI