Restriction map for
pFN21AE4364
(Linear) (Six-base) MAPSORT of: KIEE4364.seq Check: 1001 from: 1 to: 1143
KIEE4364.orf
With 182 enzymes: SgfI *
March 22, 2012 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 853 1143
Size: 853 290
AccI GT'mk_AC
Cuts at: 0 158 1143
Size: 158 985
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1143
Size: 547 596
AflIII A'CryG_T
Cuts at: 0 419 1004 1143
Size: 419 585 139
Fragments arranged by size:
585 419 139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 886 1143
Size: 886 257
AlwNI CAG_nnn'CTG
Cuts at: 0 828 1143
Size: 828 315
BanII G_rGCy'C
Cuts at: 0 994 1030 1143
Size: 994 36 113
Fragments arranged by size:
994 113 36
BbvCI CC'TCA_GC
Cuts at: 0 67 1143
Size: 67 1076
BglI GCCn_nnn'nGGC
Cuts at: 0 293 497 1143
Size: 293 204 646
Fragments arranged by size:
646 293 204
BglII A'GATC_T
Cuts at: 0 785 1143
Size: 785 358
BlpI GC'TnA_GC
Cuts at: 0 379 1143
Size: 379 764
Bme1580I G_kGCm'C
Cuts at: 0 865 1143
Size: 865 278
BmgBI CAC'GTC
Cuts at: 0 1005 1143
Size: 1005 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 875 1143
Size: 875 268
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 486 1143
Size: 486 657
Bpu10I CC'TnA_GC
Cuts at: 0 67 904 1143
Size: 67 837 239
Fragments arranged by size:
837 239 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1143
Size: 328 815
BsaAI yAC'GTr
Cuts at: 0 375 837 1143
Size: 375 462 306
Fragments arranged by size:
462 375 306
BsaHI Gr'CG_yC
Cuts at: 0 638 1143
Size: 638 505
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1081 1111 1143
Size: 1081 30 32
Fragments arranged by size:
1081 32 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 67 250 706 796 940 1143
Size: 64 3 183 456 90 144 203
Fragments arranged by size:
456 203 183 144 90 64 3
BseYI C'CCAG_C
Cuts at: 0 1023 1143
Size: 1023 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1069 1143
Size: 1069 74
BsiHKAI G_wGCw'C
Cuts at: 0 380 842 1143
Size: 380 462 301
Fragments arranged by size:
462 380 301
BsmBI CGTCTCn'nnnn_
Cuts at: 0 347 1143
Size: 347 796
Bsp1286I G_dGCh'C
Cuts at: 0 380 842 865 994 1030 1143
Size: 380 462 23 129 36 113
Fragments arranged by size:
462 380 129 113 36 23
BspHI T'CATG_A
Cuts at: 0 206 1143
Size: 206 937
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 853 1143
Size: 853 290
BstEII G'GTnAC_C
Cuts at: 0 193 1143
Size: 193 950
BstYI r'GATC_y
Cuts at: 0 785 929 1143
Size: 785 144 214
Fragments arranged by size:
785 214 144
Bsu36I CC'TnA_GG
Cuts at: 0 47 612 1143
Size: 47 565 531
Fragments arranged by size:
565 531 47
BtgI C'CryG_G
Cuts at: 0 212 288 441 642 716 986 1143
Size: 212 76 153 201 74 270 157
Fragments arranged by size:
270 212 201 157 153 76 74
BtsI GCAGTG_nn'
Cuts at: 0 432 1143
Size: 432 711
ClaI AT'CG_AT
Cuts at: 0 563 1143
Size: 563 580
EaeI y'GGCC_r
Cuts at: 0 89 399 801 1143
Size: 89 310 402 342
Fragments arranged by size:
402 342 310 89
EarI CTCTTCn'nnn_
Cuts at: 0 523 949 1143
Size: 523 426 194
Fragments arranged by size:
523 426 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 739 1143
Size: 739 404
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1143
Size: 547 596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 279 1143
Size: 279 864
HaeII r_GCGC'y
Cuts at: 0 453 1143
Size: 453 690
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 223 255 1143
Size: 127 32 64 32 888
Fragments arranged by size:
888 127 64 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 1034 1079 1143
Size: 878 156 45 64
Fragments arranged by size:
878 156 64 45
MscI TGG'CCA
Cuts at: 0 91 803 1143
Size: 91 712 340
Fragments arranged by size:
712 340 91
MslI CAynn'nnrTG
Cuts at: 0 211 715 1143
Size: 211 504 428
Fragments arranged by size:
504 428 211
MspA1I CmG'CkG
Cuts at: 0 600 982 1053 1143
Size: 600 382 71 90
Fragments arranged by size:
600 382 90 71
NcoI C'CATG_G
Cuts at: 0 716 1143
Size: 716 427
NspI r_CATG'y
Cuts at: 0 889 1143
Size: 889 254
PflMI CCAn_nnn'nTGG
Cuts at: 0 828 1143
Size: 828 315
PstI C_TGCA'G
Cuts at: 0 773 953 1143
Size: 773 180 190
Fragments arranged by size:
773 190 180
PvuII CAG'CTG
Cuts at: 0 982 1053 1143
Size: 982 71 90
Fragments arranged by size:
982 90 71
SapI GCTCTTCn'nnn_
Cuts at: 0 523 1143
Size: 523 620
ScaI AGT'ACT
Cuts at: 0 517 1143
Size: 517 626
SfcI C'TryA_G
Cuts at: 0 155 769 949 1143
Size: 155 614 180 194
Fragments arranged by size:
614 194 180 155
SmlI C'TyrA_G
Cuts at: 0 343 1143
Size: 343 800
StyI C'CwwG_G
Cuts at: 0 92 716 1143
Size: 92 624 427
Fragments arranged by size:
624 427 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 31 672 1143
Size: 31 641 471
Fragments arranged by size:
641 471 31
TatI w'GTAC_w
Cuts at: 0 515 1143
Size: 515 628
Tth111I GACn'n_nGTC
Cuts at: 0 163 1143
Size: 163 980
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI BanII BbvCI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BstEII BstYI Bsu36I BtgI
BtsI ClaI EaeI EarI EciI Eco57MI FalI HaeII
Hin4I MmeI MscI MslI MspA1I NcoI NspI PflMI
PstI PvuII SapI ScaI SfcI SmlI StyI TaqII
TatI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI
BclI BfrBI BmtI BpmI BsaI BsaBI BsaWI BsiEI
BsiWI BsmI BspEI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I BtgZI CspCI DraI
DraIII DrdI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XbaI XcmI XhoI XmaI
XmnI ZraI