Restriction map for
pFN21AE4362
(Linear) (Six-base) MAPSORT of: KIEE4362.seq Check: 3967 from: 1 to: 1140
KIEE4362.orf
With 182 enzymes: SgfI *
March 22, 2012 17:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 814 1140
Size: 814 326
AleI CACnn'nnGTG
Cuts at: 0 469 1140
Size: 469 671
ApoI r'AATT_y
Cuts at: 0 169 330 510 1033 1140
Size: 169 161 180 523 107
Fragments arranged by size:
523 180 169 161 107
AvrII C'CTAG_G
Cuts at: 0 615 1140
Size: 615 525
BanI G'GyrC_C
Cuts at: 0 4 1140
Size: 4 1136
BbsI GAAGACnn'nnnn_
Cuts at: 0 395 1140
Size: 395 745
BclI T'GATC_A
Cuts at: 0 372 1140
Size: 372 768
Bme1580I G_kGCm'C
Cuts at: 0 7 1140
Size: 7 1133
BmrI ACTGGGnnnn_n'
Cuts at: 0 184 1140
Size: 184 956
BsaBI GATnn'nnATC
Cuts at: 0 482 1140
Size: 482 658
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 949 1140
Size: 949 191
BseYI C'CCAG_C
Cuts at: 0 872 1140
Size: 872 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 1140
Size: 842 298
BsiEI CG_ry'CG
Cuts at: 0 951 1140
Size: 951 189
BsmI GAATG_Cn'
Cuts at: 0 113 1140
Size: 113 1027
Bsp1286I G_dGCh'C
Cuts at: 0 7 1140
Size: 7 1133
BspHI T'CATG_A
Cuts at: 0 102 1140
Size: 102 1038
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 205 392 418 814 1140
Size: 205 187 26 396 326
Fragments arranged by size:
396 326 205 187 26
BsrDI GCAATG_nn'
Cuts at: 0 122 1140
Size: 122 1018
BsrGI T'GTAC_A
Cuts at: 0 859 1140
Size: 859 281
BstEII G'GTnAC_C
Cuts at: 0 582 1053 1140
Size: 582 471 87
Fragments arranged by size:
582 471 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 439 1140
Size: 439 701
BstYI r'GATC_y
Cuts at: 0 829 1140
Size: 829 311
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 24 1140
Size: 24 1116
ClaI AT'CG_AT
Cuts at: 0 161 1140
Size: 161 979
EaeI y'GGCC_r
Cuts at: 0 201 948 1140
Size: 201 747 192
Fragments arranged by size:
747 201 192
EagI C'GGCC_G
Cuts at: 0 948 1140
Size: 948 192
EarI CTCTTCn'nnn_
Cuts at: 0 68 1140
Size: 68 1072
EcoO109I rG'GnC_Cy
Cuts at: 0 177 1140
Size: 177 963
EcoRV GAT'ATC
Cuts at: 0 480 1140
Size: 480 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 510 542 1140
Size: 510 32 598
Fragments arranged by size:
598 510 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1140
Size: 961 179
MscI TGG'CCA
Cuts at: 0 203 1140
Size: 203 937
MslI CAynn'nnrTG
Cuts at: 0 146 291 469 1140
Size: 146 145 178 671
Fragments arranged by size:
671 178 146 145
PasI CC'CwG_GG
Cuts at: 0 720 1140
Size: 720 420
PflMI CCAn_nnn'nTGG
Cuts at: 0 44 1140
Size: 44 1096
PfoI T'CCnGG_A
Cuts at: 0 767 1140
Size: 767 373
PshAI GACnn'nnGTC
Cuts at: 0 250 1140
Size: 250 890
PstI C_TGCA'G
Cuts at: 0 216 1140
Size: 216 924
SexAI A'CCwGG_T
Cuts at: 0 258 1140
Size: 258 882
SfcI C'TryA_G
Cuts at: 0 212 1140
Size: 212 928
StyI C'CwwG_G
Cuts at: 0 8 95 578 615 791 1107 1140
Size: 8 87 483 37 176 316 33
Fragments arranged by size:
483 316 176 87 37 33 8
TatI w'GTAC_w
Cuts at: 0 448 461 859 883 1140
Size: 448 13 398 24 257
Fragments arranged by size:
448 398 257 24 13
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 798 1140
Size: 798 342
Enzymes that do cut:
AarI AleI ApoI AvrII BanI BbsI BclI Bme1580I
BmrI BsaBI BseRI BseYI BsgI BsiEI BsmI Bsp1286I
BspHI BspMI BsrDI BsrGI BstEII BstXI BstYI BtgZI
ClaI EaeI EagI EarI EcoO109I EcoRV Hin4I MmeI
MscI MslI PasI PflMI PfoI PshAI PstI SexAI
SfcI StyI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI BaeI BamHI BanII BbeI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgI BtsI CspCI
DraI DraIII DrdI EciI EcoICRI Eco57MI EcoNI EcoRI
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI