Restriction map for
pFN21AE4358
(Linear) (Six-base) MAPSORT of: KIEE4358.seq Check: 4054 from: 1 to: 1125
KIEE4358.orf
With 182 enzymes: SgfI *
March 22, 2012 17:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 1057 1125
Size: 164 893 68
Fragments arranged by size:
893 164 68
AfeI AGC'GCT
Cuts at: 0 970 1125
Size: 970 155
AgeI A'CCGG_T
Cuts at: 0 767 1125
Size: 767 358
AleI CACnn'nnGTG
Cuts at: 0 886 1125
Size: 886 239
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 561 1125
Size: 561 564
AlwNI CAG_nnn'CTG
Cuts at: 0 403 1125
Size: 403 722
ApoI r'AATT_y
Cuts at: 0 959 985 1078 1125
Size: 959 26 93 47
Fragments arranged by size:
959 93 47 26
BanII G_rGCy'C
Cuts at: 0 45 1125
Size: 45 1080
BbvCI CC'TCA_GC
Cuts at: 0 550 863 1125
Size: 550 313 262
Fragments arranged by size:
550 313 262
BglI GCCn_nnn'nGGC
Cuts at: 0 899 1097 1125
Size: 899 198 28
Fragments arranged by size:
899 198 28
Bme1580I G_kGCm'C
Cuts at: 0 170 842 1125
Size: 170 672 283
Fragments arranged by size:
672 283 170
BmrI ACTGGGnnnn_n'
Cuts at: 0 97 157 1125
Size: 97 60 968
Fragments arranged by size:
968 97 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 615 1125
Size: 615 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 1125
Size: 956 169
Bpu10I CC'TnA_GC
Cuts at: 0 550 782 863 965 1125
Size: 550 232 81 102 160
Fragments arranged by size:
550 232 160 102 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 846 1125
Size: 846 279
BsaBI GATnn'nnATC
Cuts at: 0 928 1125
Size: 928 197
BsaHI Gr'CG_yC
Cuts at: 0 833 1125
Size: 833 292
BsaWI w'CCGG_w
Cuts at: 0 767 1125
Size: 767 358
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 199 1125
Size: 196 3 926
Fragments arranged by size:
926 196 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 1125
Size: 557 568
BsiHKAI G_wGCw'C
Cuts at: 0 299 545 1125
Size: 299 246 580
Fragments arranged by size:
580 299 246
BsmI GAATG_Cn'
Cuts at: 0 389 1125
Size: 389 736
Bsp1286I G_dGCh'C
Cuts at: 0 45 170 299 545 842 1125
Size: 45 125 129 246 297 283
Fragments arranged by size:
297 283 246 129 125 45
BsrFI r'CCGG_y
Cuts at: 0 767 788 1125
Size: 767 21 337
Fragments arranged by size:
767 337 21
BsrGI T'GTAC_A
Cuts at: 0 251 926 1125
Size: 251 675 199
Fragments arranged by size:
675 251 199
BstXI CCAn_nnnn'nTGG
Cuts at: 0 427 753 888 1057 1125
Size: 427 326 135 169 68
Fragments arranged by size:
427 326 169 135 68
BstYI r'GATC_y
Cuts at: 0 16 1125
Size: 16 1109
BtgI C'CryG_G
Cuts at: 0 227 700 1125
Size: 227 473 425
Fragments arranged by size:
473 425 227
BtsI GCAGTG_nn'
Cuts at: 0 545 766 1125
Size: 545 221 359
Fragments arranged by size:
545 359 221
EarI CTCTTCn'nnn_
Cuts at: 0 98 143 173 236 644 1125
Size: 98 45 30 63 408 481
Fragments arranged by size:
481 408 98 63 45 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 956 1057 1125
Size: 164 792 101 68
Fragments arranged by size:
792 164 101 68
EcoO109I rG'GnC_Cy
Cuts at: 0 91 1125
Size: 91 1034
EcoRI G'AATT_C
Cuts at: 0 959 1078 1125
Size: 959 119 47
Fragments arranged by size:
959 119 47
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 399 1125
Size: 399 726
HaeII r_GCGC'y
Cuts at: 0 972 1125
Size: 972 153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1091 1125
Size: 253 838 34
Fragments arranged by size:
838 253 34
MslI CAynn'nnrTG
Cuts at: 0 751 886 919 1055 1125
Size: 751 135 33 136 70
Fragments arranged by size:
751 136 135 70 33
NcoI C'CATG_G
Cuts at: 0 227 1125
Size: 227 898
NdeI CA'TA_TG
Cuts at: 0 288 994 1125
Size: 288 706 131
Fragments arranged by size:
706 288 131
NspI r_CATG'y
Cuts at: 0 161 1125
Size: 161 964
PflMI CCAn_nnn'nTGG
Cuts at: 0 228 1026 1125
Size: 228 798 99
Fragments arranged by size:
798 228 99
PfoI T'CCnGG_A
Cuts at: 0 978 1125
Size: 978 147
PpuMI rG'GwC_Cy
Cuts at: 0 91 1125
Size: 91 1034
SanDI GG'GwC_CC
Cuts at: 0 91 1125
Size: 91 1034
SapI GCTCTTCn'nnn_
Cuts at: 0 143 644 1125
Size: 143 501 481
Fragments arranged by size:
501 481 143
SfcI C'TryA_G
Cuts at: 0 499 1125
Size: 499 626
SmlI C'TyrA_G
Cuts at: 0 825 1125
Size: 825 300
StyI C'CwwG_G
Cuts at: 0 227 1125
Size: 227 898
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 150 523 1125
Size: 150 373 602
Fragments arranged by size:
602 373 150
TatI w'GTAC_w
Cuts at: 0 251 482 926 1105 1125
Size: 251 231 444 179 20
Fragments arranged by size:
444 251 231 179 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 8 40 1125
Size: 8 32 1085
Fragments arranged by size:
1085 32 8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 234 1125
Size: 234 891
Enzymes that do cut:
AcuI AfeI AgeI AleI AlfI AlwNI ApoI BanII
BbvCI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaWI BseRI BsgI BsiHKAI BsmI Bsp1286I
BsrFI BsrGI BstXI BstYI BtgI BtsI EarI Eco57MI
EcoO109I EcoRI FalI HaeII MmeI MslI NcoI NdeI
NspI PflMI PfoI PpuMI SanDI SapI SfcI SmlI
StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BsaI BsaAI BsaXI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
MspA1I NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI