Restriction map for
pFN21AE4357
(Linear) (Six-base) MAPSORT of: KIEE4357.seq Check: 6807 from: 1 to: 1122
KIEE4357.orf
With 182 enzymes: SgfI *
March 22, 2012 17:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 815 1122
Size: 815 307
AflIII A'CryG_T
Cuts at: 0 467 1122
Size: 467 655
AhdI GACnn_n'nnGTC
Cuts at: 0 915 1122
Size: 915 207
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 494 526 1122
Size: 494 32 596
Fragments arranged by size:
596 494 32
ApaI G_GGCC'C
Cuts at: 0 409 524 565 1122
Size: 409 115 41 557
Fragments arranged by size:
557 409 115 41
BanII G_rGCy'C
Cuts at: 0 409 460 524 565 1122
Size: 409 51 64 41 557
Fragments arranged by size:
557 409 64 51 41
BclI T'GATC_A
Cuts at: 0 362 1122
Size: 362 760
Bme1580I G_kGCm'C
Cuts at: 0 409 524 565 1122
Size: 409 115 41 557
Fragments arranged by size:
557 409 115 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 335 1122
Size: 335 787
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 747 1122
Size: 747 375
BsaI GGTCTCn'nnnn_
Cuts at: 0 243 1122
Size: 243 879
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 74 104 416 446 494 524 1122
Size: 74 30 312 30 48 30 598
Fragments arranged by size:
598 312 74 48 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 844 1122
Size: 844 278
BseYI C'CCAG_C
Cuts at: 0 398 402 1122
Size: 398 4 720
Fragments arranged by size:
720 398 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 793 1122
Size: 163 630 329
Fragments arranged by size:
630 329 163
BsiEI CG_ry'CG
Cuts at: 0 776 1122
Size: 776 346
BsiHKAI G_wGCw'C
Cuts at: 0 460 883 1122
Size: 460 423 239
Fragments arranged by size:
460 423 239
BsmBI CGTCTCn'nnnn_
Cuts at: 0 783 1122
Size: 783 339
Bsp1286I G_dGCh'C
Cuts at: 0 409 460 524 565 883 1122
Size: 409 51 64 41 318 239
Fragments arranged by size:
409 318 239 64 51 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 815 1122
Size: 815 307
BsrGI T'GTAC_A
Cuts at: 0 1070 1122
Size: 1070 52
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 623 1122
Size: 417 206 499
Fragments arranged by size:
499 417 206
BstYI r'GATC_y
Cuts at: 0 1099 1122
Size: 1099 23
Bsu36I CC'TnA_GG
Cuts at: 0 968 1122
Size: 968 154
BtgI C'CryG_G
Cuts at: 0 410 573 1122
Size: 410 163 549
Fragments arranged by size:
549 410 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 856 1122
Size: 856 266
BtsI GCAGTG_nn'
Cuts at: 0 148 643 996 1122
Size: 148 495 353 126
Fragments arranged by size:
495 353 148 126
EaeI y'GGCC_r
Cuts at: 0 58 735 1122
Size: 58 677 387
Fragments arranged by size:
677 387 58
EarI CTCTTCn'nnn_
Cuts at: 0 119 850 862 1122
Size: 119 731 12 260
Fragments arranged by size:
731 260 119 12
EcoICRI GAG'CTC
Cuts at: 0 458 1122
Size: 458 664
EcoO109I rG'GnC_Cy
Cuts at: 0 53 287 406 520 562 581 824 1122
Size: 53 234 119 114 42 19 243 298
Fragments arranged by size:
298 243 234 119 114 53 42 19
EcoRV GAT'ATC
Cuts at: 0 1062 1122
Size: 1062 60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 267 1122
Size: 267 855
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 354 386 901 933 1084 1116
Size: 223 32 99 32 515 32 151 32
Cuts at: 1116 1122
Size: 6
Fragments arranged by size:
515 223 151 99 32 32 32 32
6
HincII GTy'rAC
Cuts at: 0 531 1122
Size: 531 591
HindIII A'AGCT_T
Cuts at: 0 740 1122
Size: 740 382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 908 1122
Size: 796 112 214
Fragments arranged by size:
796 214 112
MscI TGG'CCA
Cuts at: 0 60 737 1122
Size: 60 677 385
Fragments arranged by size:
677 385 60
MslI CAynn'nnrTG
Cuts at: 0 230 621 1122
Size: 230 391 501
Fragments arranged by size:
501 391 230
MspA1I CmG'CkG
Cuts at: 0 402 1122
Size: 402 720
NspI r_CATG'y
Cuts at: 0 471 788 1122
Size: 471 317 334
Fragments arranged by size:
471 334 317
PciI A'CATG_T
Cuts at: 0 467 1122
Size: 467 655
PfoI T'CCnGG_A
Cuts at: 0 451 1122
Size: 451 671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 494 526 1122
Size: 494 32 596
Fragments arranged by size:
596 494 32
PpuMI rG'GwC_Cy
Cuts at: 0 287 581 824 1122
Size: 287 294 243 298
Fragments arranged by size:
298 294 287 243
PspOMI G'GGCC_C
Cuts at: 0 405 520 561 1122
Size: 405 115 41 561
Fragments arranged by size:
561 405 115 41
PstI C_TGCA'G
Cuts at: 0 50 143 448 519 924 1122
Size: 50 93 305 71 405 198
Fragments arranged by size:
405 305 198 93 71 50
PvuII CAG'CTG
Cuts at: 0 402 1122
Size: 402 720
SacI G_AGCT'C
Cuts at: 0 460 1122
Size: 460 662
SbfI CC_TGCA'GG
Cuts at: 0 519 1122
Size: 519 603
SfcI C'TryA_G
Cuts at: 0 46 139 291 444 515 585 920 1122
Size: 46 93 152 153 71 70 335 202
Fragments arranged by size:
335 202 153 152 93 71 70 46
SmlI C'TyrA_G
Cuts at: 0 762 1122
Size: 762 360
SphI G_CATG'C
Cuts at: 0 788 1122
Size: 788 334
StyI C'CwwG_G
Cuts at: 0 242 524 1122
Size: 242 282 598
Fragments arranged by size:
598 282 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 116 586 705 1122
Size: 116 470 119 417
Fragments arranged by size:
470 417 119 116
TatI w'GTAC_w
Cuts at: 0 1070 1122
Size: 1070 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 1065 1097 1122
Size: 416 32 617 32 25
Fragments arranged by size:
617 416 32 32 25
Tth111I GACn'n_nGTC
Cuts at: 0 438 1122
Size: 438 684
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 348 1122
Size: 348 774
XmnI GAAnn'nnTTC
Cuts at: 0 84 669 1122
Size: 84 585 453
Fragments arranged by size:
585 453 84
Enzymes that do cut:
AarI AflIII AhdI AloI ApaI BanII BclI Bme1580I
BmrI BpuEI BsaI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrGI BstXI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EarI EcoICRI EcoO109I EcoRV
FalI Hin4I HincII HindIII MmeI MscI MslI MspA1I
NspI PciI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SbfI SfcI SmlI SphI StyI TaqII TatI
TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII
DrdI EagI EciI Eco57MI EcoNI EcoRI FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI XbaI XhoI XmaI ZraI