Restriction map for
pFN21AE4352
(Linear) (Six-base) MAPSORT of: KIEE4352.seq Check: 8281 from: 1 to: 1107
KIEE4352.orf
With 182 enzymes: SgfI *
March 22, 2012 17:48 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 215 884 1107
Size: 215 669 223
Fragments arranged by size:
669 223 215
ApaI G_GGCC'C
Cuts at: 0 672 1107
Size: 672 435
ApaLI G'TGCA_C
Cuts at: 0 822 1107
Size: 822 285
AvaI C'yCGr_G
Cuts at: 0 756 1107
Size: 756 351
BanI G'GyrC_C
Cuts at: 0 24 148 429 510 952 1107
Size: 24 124 281 81 442 155
Fragments arranged by size:
442 281 155 124 81 24
BanII G_rGCy'C
Cuts at: 0 473 672 1107
Size: 473 199 435
Fragments arranged by size:
473 435 199
BbeI G_GCGC'C
Cuts at: 0 28 433 1107
Size: 28 405 674
Fragments arranged by size:
674 405 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 416 1029 1107
Size: 248 168 613 78
Fragments arranged by size:
613 248 168 78
BclI T'GATC_A
Cuts at: 0 833 1107
Size: 833 274
BfrBI ATG'CAT
Cuts at: 0 1089 1107
Size: 1089 18
Bme1580I G_kGCm'C
Cuts at: 0 515 672 826 957 1107
Size: 515 157 154 131 150
Fragments arranged by size:
515 157 154 150 131
BmrI ACTGGGnnnn_n'
Cuts at: 0 675 836 1107
Size: 675 161 271
Fragments arranged by size:
675 271 161
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 990 1107
Size: 990 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 1107
Size: 157 950
BsaI GGTCTCn'nnnn_
Cuts at: 0 334 1107
Size: 334 773
BsaBI GATnn'nnATC
Cuts at: 0 309 1107
Size: 309 798
BsaHI Gr'CG_yC
Cuts at: 0 25 430 604 1025 1107
Size: 25 405 174 421 82
Fragments arranged by size:
421 405 174 82 25
BseYI C'CCAG_C
Cuts at: 0 627 720 1107
Size: 627 93 387
Fragments arranged by size:
627 387 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 379 1107
Size: 273 106 728
Fragments arranged by size:
728 273 106
BsiHKAI G_wGCw'C
Cuts at: 0 473 826 1107
Size: 473 353 281
Fragments arranged by size:
473 353 281
Bsp1286I G_dGCh'C
Cuts at: 0 473 515 672 826 957 1107
Size: 473 42 157 154 131 150
Fragments arranged by size:
473 157 154 150 131 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 512 1107
Size: 512 595
BsrDI GCAATG_nn'
Cuts at: 0 866 1107
Size: 866 241
BsrFI r'CCGG_y
Cuts at: 0 154 1107
Size: 154 953
BsrGI T'GTAC_A
Cuts at: 0 186 1107
Size: 186 921
BssHII G'CGCG_C
Cuts at: 0 180 1107
Size: 180 927
BstEII G'GTnAC_C
Cuts at: 0 384 1107
Size: 384 723
BstXI CCAn_nnnn'nTGG
Cuts at: 0 300 441 1107
Size: 300 141 666
Fragments arranged by size:
666 300 141
BstYI r'GATC_y
Cuts at: 0 986 1107
Size: 986 121
Bsu36I CC'TnA_GG
Cuts at: 0 457 787 1107
Size: 457 330 320
Fragments arranged by size:
457 330 320
BtgI C'CryG_G
Cuts at: 0 111 1107
Size: 111 996
BtsI GCAGTG_nn'
Cuts at: 0 130 1107
Size: 130 977
DrdI GACnn_nn'nnGTC
Cuts at: 0 970 1107
Size: 970 137
EaeI y'GGCC_r
Cuts at: 0 41 1107
Size: 41 1066
EcoICRI GAG'CTC
Cuts at: 0 471 1107
Size: 471 636
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 1107
Size: 157 950
EcoO109I rG'GnC_Cy
Cuts at: 0 783 1107
Size: 783 324
HaeII r_GCGC'y
Cuts at: 0 28 40 433 1107
Size: 28 12 393 674
Fragments arranged by size:
674 393 28 12
KasI G'GCGC_C
Cuts at: 0 24 429 1107
Size: 24 405 678
Fragments arranged by size:
678 405 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 1107
Size: 338 769
MslI CAynn'nnrTG
Cuts at: 0 688 829 1107
Size: 688 141 278
Fragments arranged by size:
688 278 141
MspA1I CmG'CkG
Cuts at: 0 31 113 287 394 1107
Size: 31 82 174 107 713
Fragments arranged by size:
713 174 107 82 31
NarI GG'CG_CC
Cuts at: 0 25 430 1107
Size: 25 405 677
Fragments arranged by size:
677 405 25
NsiI A_TGCA'T
Cuts at: 0 1091 1107
Size: 1091 16
PflMI CCAn_nnn'nTGG
Cuts at: 0 1074 1107
Size: 1074 33
PfoI T'CCnGG_A
Cuts at: 0 473 767 1107
Size: 473 294 340
Fragments arranged by size:
473 340 294
PpuMI rG'GwC_Cy
Cuts at: 0 783 1107
Size: 783 324
PspOMI G'GGCC_C
Cuts at: 0 668 1107
Size: 668 439
PvuII CAG'CTG
Cuts at: 0 287 394 1107
Size: 287 107 713
Fragments arranged by size:
713 287 107
SacI G_AGCT'C
Cuts at: 0 473 1107
Size: 473 634
SacII CC_GC'GG
Cuts at: 0 114 1107
Size: 114 993
SfoI GGC'GCC
Cuts at: 0 26 431 1107
Size: 26 405 676
Fragments arranged by size:
676 405 26
SmaI CCC'GGG
Cuts at: 0 758 1107
Size: 758 349
StuI AGG'CCT
Cuts at: 0 142 1107
Size: 142 965
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 100 1107
Size: 100 1007
TatI w'GTAC_w
Cuts at: 0 186 1107
Size: 186 921
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 934 1107
Size: 934 173
XmaI C'CCGG_G
Cuts at: 0 756 1107
Size: 756 351
Enzymes that do cut:
AlfI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BclI BfrBI Bme1580I BmrI BplI BpmI BsaI BsaBI
BsaHI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssHII BstEII BstXI BstYI Bsu36I BtgI BtsI
DrdI EaeI EcoICRI Eco57MI EcoO109I HaeII KasI MmeI
MslI MspA1I NarI NsiI PflMI PfoI PpuMI PspOMI
PvuII SacI SacII SfoI SmaI StuI TaqII TatI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BglI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaAI
BsaWI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BssSI BstAPI BstBI BstZ17I BtgZI ClaI
CspCI DraI DraIII EagI EarI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MscI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StyI SwaI TstI Tth111I XbaI XhoI
XmnI ZraI