Restriction map for
pFN21AE4350
(Linear) (Six-base) MAPSORT of: KIEE4350.seq Check: 7796 from: 1 to: 1095
KIEE4350.orf
With 182 enzymes: SgfI *
March 22, 2012 17:48 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 315 390 720 1095
Size: 271 44 75 330 375
Fragments arranged by size:
375 330 271 75 44
AleI CACnn'nnGTG
Cuts at: 0 306 1095
Size: 306 789
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 916 1095
Size: 916 179
AlwNI CAG_nnn'CTG
Cuts at: 0 525 961 1095
Size: 525 436 134
Fragments arranged by size:
525 436 134
ApaI G_GGCC'C
Cuts at: 0 11 1095
Size: 11 1084
AvaI C'yCGr_G
Cuts at: 0 27 136 446 504 1095
Size: 27 109 310 58 591
Fragments arranged by size:
591 310 109 58 27
BamHI G'GATC_C
Cuts at: 0 261 272 1095
Size: 261 11 823
Fragments arranged by size:
823 261 11
BanI G'GyrC_C
Cuts at: 0 300 325 1095
Size: 300 25 770
Fragments arranged by size:
770 300 25
BanII G_rGCy'C
Cuts at: 0 11 20 370 658 715 968 986 1004
Size: 11 9 350 288 57 253 18 18
Cuts at: 1004 1028 1095
Size: 24 67
Fragments arranged by size:
350 288 253 67 57 24 18 18
11 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 938 1095
Size: 938 157
BglI GCCn_nnn'nGGC
Cuts at: 0 748 1095
Size: 748 347
BglII A'GATC_T
Cuts at: 0 386 1095
Size: 386 709
Bme1580I G_kGCm'C
Cuts at: 0 11 287 303 511 1095
Size: 11 276 16 208 584
Fragments arranged by size:
584 276 208 16 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 718 1095
Size: 718 377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1023 1036 1095
Size: 1023 13 59
Fragments arranged by size:
1023 59 13
Bpu10I CC'TnA_GC
Cuts at: 0 962 974 980 1095
Size: 962 12 6 115
Fragments arranged by size:
962 115 12 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1095
Size: 1018 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 307 465 1066 1095
Size: 307 158 601 29
Fragments arranged by size:
601 307 158 29
BsaBI GATnn'nnATC
Cuts at: 0 232 1095
Size: 232 863
BsaHI Gr'CG_yC
Cuts at: 0 107 1095
Size: 107 988
BsaWI w'CCGG_w
Cuts at: 0 95 818 1095
Size: 95 723 277
Fragments arranged by size:
723 277 95
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 646 955 1095
Size: 646 309 140
Fragments arranged by size:
646 309 140
BseYI C'CCAG_C
Cuts at: 0 693 784 967 991 1045 1051 1095
Size: 693 91 183 24 54 6 44
Fragments arranged by size:
693 183 91 54 44 24 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1095
Size: 571 524
BsiEI CG_ry'CG
Cuts at: 0 882 1095
Size: 882 213
BsiHKAI G_wGCw'C
Cuts at: 0 370 1095
Size: 370 725
Bsp1286I G_dGCh'C
Cuts at: 0 11 20 287 303 370 511 658 715
Size: 11 9 267 16 67 141 147 57
Cuts at: 715 968 986 1004 1028 1095
Size: 253 18 18 24 67
Fragments arranged by size:
267 253 147 141 67 67 57 24
18 18 16 11 9
BspEI T'CCGG_A
Cuts at: 0 818 1095
Size: 818 277
BspHI T'CATG_A
Cuts at: 0 362 1095
Size: 362 733
BsrBI CCG'CTC
Cuts at: 0 278 297 1095
Size: 278 19 798
Fragments arranged by size:
798 278 19
BstXI CCAn_nnnn'nTGG
Cuts at: 0 792 1095
Size: 792 303
BstYI r'GATC_y
Cuts at: 0 23 261 272 386 957 1095
Size: 23 238 11 114 571 138
Fragments arranged by size:
571 238 138 114 23 11
Bsu36I CC'TnA_GG
Cuts at: 0 526 1095
Size: 526 569
BtgI C'CryG_G
Cuts at: 0 659 1095
Size: 659 436
DrdI GACnn_nn'nnGTC
Cuts at: 0 457 1095
Size: 457 638
EaeI y'GGCC_r
Cuts at: 0 1059 1095
Size: 1059 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 169 1095
Size: 169 926
EcoICRI GAG'CTC
Cuts at: 0 368 1095
Size: 368 727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 315 390 720 1023 1036 1095
Size: 271 44 75 330 303 13 59
Fragments arranged by size:
330 303 271 75 59 44 13
EcoO109I rG'GnC_Cy
Cuts at: 0 501 809 1095
Size: 501 308 286
Fragments arranged by size:
501 308 286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 420 867 1095
Size: 420 447 228
Fragments arranged by size:
447 420 228
HaeII r_GCGC'y
Cuts at: 0 743 1095
Size: 743 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 357 389 1095
Size: 43 32 282 32 706
Fragments arranged by size:
706 282 43 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 1095
Size: 788 307
MslI CAynn'nnrTG
Cuts at: 0 306 1095
Size: 306 789
MspA1I CmG'CkG
Cuts at: 0 693 1095
Size: 693 402
PasI CC'CwG_GG
Cuts at: 0 923 1095
Size: 923 172
PfoI T'CCnGG_A
Cuts at: 0 268 338 1095
Size: 268 70 757
Fragments arranged by size:
757 268 70
PpuMI rG'GwC_Cy
Cuts at: 0 501 1095
Size: 501 594
PshAI GACnn'nnGTC
Cuts at: 0 800 1095
Size: 800 295
PspOMI G'GGCC_C
Cuts at: 0 7 1095
Size: 7 1088
PstI C_TGCA'G
Cuts at: 0 641 1095
Size: 641 454
PvuI CG_AT'CG
Cuts at: 0 882 1095
Size: 882 213
SacI G_AGCT'C
Cuts at: 0 370 1095
Size: 370 725
SfcI C'TryA_G
Cuts at: 0 637 1095
Size: 637 458
SmaI CCC'GGG
Cuts at: 0 29 448 506 1095
Size: 29 419 58 589
Fragments arranged by size:
589 419 58 29
SmlI C'TyrA_G
Cuts at: 0 136 997 1095
Size: 136 861 98
Fragments arranged by size:
861 136 98
StuI AGG'CCT
Cuts at: 0 400 1066 1095
Size: 400 666 29
Fragments arranged by size:
666 400 29
StyI C'CwwG_G
Cuts at: 0 395 413 419 1095
Size: 395 18 6 676
Fragments arranged by size:
676 395 18 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 178 313 1095
Size: 178 135 782
Fragments arranged by size:
782 178 135
TatI w'GTAC_w
Cuts at: 0 128 347 1095
Size: 128 219 748
Fragments arranged by size:
748 219 128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1011 1095
Size: 1011 84
XhoI C'TCGA_G
Cuts at: 0 136 1095
Size: 136 959
XmaI C'CCGG_G
Cuts at: 0 27 446 504 1095
Size: 27 419 58 591
Fragments arranged by size:
591 419 58 27
XmnI GAAnn'nnTTC
Cuts at: 0 850 1095
Size: 850 245
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI AvaI BamHI BanI
BanII BbsI BglI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BsrBI BstXI BstYI
Bsu36I BtgI DrdI EaeI EciI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I MmeI MslI MspA1I PasI PfoI
PpuMI PshAI PspOMI PstI PvuI SacI SfcI SmaI
SmlI StuI StyI TaqII TatI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI
BlpI BmgBI BmtI BplI BsaAI BsaXI BsiWI BsmI
BsmBI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII EagI EarI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PsiI PspXI PsrI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI ZraI