Restriction map for
pFN21AE4348
(Linear) (Six-base) MAPSORT of: KIEE4348.seq Check: 5307 from: 1 to: 1092
KIEE4348.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AccI GT'mk_AC
Cuts at: 0 326 638 1092
Size: 326 312 454
Fragments arranged by size:
454 326 312
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 706 898 952 982 1092
Size: 475 231 192 54 30 110
Fragments arranged by size:
475 231 192 110 54 30
AfeI AGC'GCT
Cuts at: 0 400 601 767 1092
Size: 400 201 166 325
Fragments arranged by size:
400 325 201 166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 941 1092
Size: 941 151
AlwNI CAG_nnn'CTG
Cuts at: 0 186 530 1092
Size: 186 344 562
Fragments arranged by size:
562 344 186
ApaI G_GGCC'C
Cuts at: 0 891 1092
Size: 891 201
BanI G'GyrC_C
Cuts at: 0 72 142 676 1092
Size: 72 70 534 416
Fragments arranged by size:
534 416 72 70
BanII G_rGCy'C
Cuts at: 0 891 915 996 1092
Size: 891 24 81 96
Fragments arranged by size:
891 96 81 24
BbvCI CC'TCA_GC
Cuts at: 0 477 1092
Size: 477 615
BglI GCCn_nnn'nGGC
Cuts at: 0 919 934 1092
Size: 919 15 158
Fragments arranged by size:
919 158 15
Bme1580I G_kGCm'C
Cuts at: 0 408 554 785 871 891 1092
Size: 408 146 231 86 20 201
Fragments arranged by size:
408 231 201 146 86 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 119 402 806 1092
Size: 119 283 404 286
Fragments arranged by size:
404 286 283 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 1092
Size: 836 256
Bpu10I CC'TnA_GC
Cuts at: 0 477 1092
Size: 477 615
BsaHI Gr'CG_yC
Cuts at: 0 273 838 1092
Size: 273 565 254
Fragments arranged by size:
565 273 254
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 823 853 1092
Size: 823 30 239
Fragments arranged by size:
823 239 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 754 844 847 991 1092
Size: 172 582 90 3 144 101
Fragments arranged by size:
582 172 144 101 90 3
BseYI C'CCAG_C
Cuts at: 0 1045 1092
Size: 1045 47
BsiEI CG_ry'CG
Cuts at: 0 204 758 909 1092
Size: 204 554 151 183
Fragments arranged by size:
554 204 183 151
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1080 1092
Size: 1080 12
Bsp1286I G_dGCh'C
Cuts at: 0 408 554 785 871 891 915 996 1092
Size: 408 146 231 86 20 24 81 96
Fragments arranged by size:
408 231 146 96 86 81 24 20
BspHI T'CATG_A
Cuts at: 0 735 1092
Size: 735 357
BsrBI CCG'CTC
Cuts at: 0 1037 1092
Size: 1037 55
BsrDI GCAATG_nn'
Cuts at: 0 116 1092
Size: 116 976
BsrFI r'CCGG_y
Cuts at: 0 164 1092
Size: 164 928
BstEII G'GTnAC_C
Cuts at: 0 699 1092
Size: 699 393
BstXI CCAn_nnnn'nTGG
Cuts at: 0 510 541 1092
Size: 510 31 551
Fragments arranged by size:
551 510 31
BstYI r'GATC_y
Cuts at: 0 569 1092
Size: 569 523
BtgI C'CryG_G
Cuts at: 0 749 1092
Size: 749 343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 39 1092
Size: 39 1053
EaeI y'GGCC_r
Cuts at: 0 906 998 1092
Size: 906 92 94
Fragments arranged by size:
906 94 92
EagI C'GGCC_G
Cuts at: 0 906 1092
Size: 906 186
EarI CTCTTCn'nnn_
Cuts at: 0 241 1075 1092
Size: 241 834 17
Fragments arranged by size:
834 241 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 277 1092
Size: 277 815
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 706 836 898 952 982 1092
Size: 475 231 130 62 54 30 110
Fragments arranged by size:
475 231 130 110 62 54 30
EcoO109I rG'GnC_Cy
Cuts at: 0 888 1092
Size: 888 204
FspI TGC'GCA
Cuts at: 0 971 1092
Size: 971 121
HaeII r_GCGC'y
Cuts at: 0 171 402 603 769 1092
Size: 171 231 201 166 323
Fragments arranged by size:
323 231 201 171 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 823 855 1092
Size: 376 32 415 32 237
Fragments arranged by size:
415 376 237 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 1092
Size: 362 730
MscI TGG'CCA
Cuts at: 0 1000 1092
Size: 1000 92
MspA1I CmG'CkG
Cuts at: 0 183 196 790 1092
Size: 183 13 594 302
Fragments arranged by size:
594 302 183 13
NcoI C'CATG_G
Cuts at: 0 749 1092
Size: 749 343
PasI CC'CwG_GG
Cuts at: 0 884 1092
Size: 884 208
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 1092
Size: 381 711
PspOMI G'GGCC_C
Cuts at: 0 887 1092
Size: 887 205
PstI C_TGCA'G
Cuts at: 0 99 134 920 1092
Size: 99 35 786 172
Fragments arranged by size:
786 172 99 35
PvuII CAG'CTG
Cuts at: 0 183 196 1092
Size: 183 13 896
Fragments arranged by size:
896 183 13
SbfI CC_TGCA'GG
Cuts at: 0 920 1092
Size: 920 172
SfcI C'TryA_G
Cuts at: 0 95 130 916 1092
Size: 95 35 786 176
Fragments arranged by size:
786 176 95 35
StuI AGG'CCT
Cuts at: 0 922 937 1092
Size: 922 15 155
Fragments arranged by size:
922 155 15
StyI C'CwwG_G
Cuts at: 0 299 375 749 1092
Size: 299 76 374 343
Fragments arranged by size:
374 343 299 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 388 631 1092
Size: 388 243 461
Fragments arranged by size:
461 388 243
TatI w'GTAC_w
Cuts at: 0 410 1025 1092
Size: 410 615 67
Fragments arranged by size:
615 410 67
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 378 382 1092
Size: 378 4 710
Fragments arranged by size:
710 378 4
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI ApaI BanI BanII
BbvCI BglI Bme1580I BmrI BpmI Bpu10I BsaHI BsaXI
BseRI BseYI BsiEI BsmBI Bsp1286I BspHI BsrBI BsrDI
BsrFI BstEII BstXI BstYI BtgI BtgZI EaeI EagI
EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I MmeI
MscI MspA1I NcoI PasI PflMI PspOMI PstI PvuII
SbfI SfcI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BsgI BsiHKAI BsiWI BsmI BspEI
BspMI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI