Restriction map for
pFN21AE4347
(Linear) (Six-base) MAPSORT of: KIEE4347.seq Check: 4545 from: 1 to: 1089
KIEE4347.orf
With 182 enzymes: SgfI *
March 22, 2012 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 177 1089
Size: 177 912
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1089
Size: 376 713
AleI CACnn'nnGTG
Cuts at: 0 146 1089
Size: 146 943
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1063 1089
Size: 1063 26
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 977 1009 1089
Size: 977 32 80
Fragments arranged by size:
977 80 32
ApaI G_GGCC'C
Cuts at: 0 614 1089
Size: 614 475
ApoI r'AATT_y
Cuts at: 0 700 1089
Size: 700 389
AvrII C'CTAG_G
Cuts at: 0 195 1089
Size: 195 894
BanI G'GyrC_C
Cuts at: 0 165 904 1030 1089
Size: 165 739 126 59
Fragments arranged by size:
739 165 126 59
BanII G_rGCy'C
Cuts at: 0 477 614 1089
Size: 477 137 475
Fragments arranged by size:
477 475 137
BbsI GAAGACnn'nnnn_
Cuts at: 0 95 1089
Size: 95 994
BlpI GC'TnA_GC
Cuts at: 0 190 257 1089
Size: 190 67 832
Fragments arranged by size:
832 190 67
Bme1580I G_kGCm'C
Cuts at: 0 276 614 909 1089
Size: 276 338 295 180
Fragments arranged by size:
338 295 276 180
BmrI ACTGGGnnnn_n'
Cuts at: 0 323 586 608 1089
Size: 323 263 22 481
Fragments arranged by size:
481 323 263 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 241 283 1089
Size: 95 146 42 806
Fragments arranged by size:
806 146 95 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 34 337 368 634 1089
Size: 34 303 31 266 455
Fragments arranged by size:
455 303 266 34 31
BsaHI Gr'CG_yC
Cuts at: 0 433 1089
Size: 433 656
BsaWI w'CCGG_w
Cuts at: 0 443 915 1089
Size: 443 472 174
Fragments arranged by size:
472 443 174
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 238 560 652 1089
Size: 97 141 322 92 437
Fragments arranged by size:
437 322 141 97 92
BseYI C'CCAG_C
Cuts at: 0 558 1004 1089
Size: 558 446 85
Fragments arranged by size:
558 446 85
BsiEI CG_ry'CG
Cuts at: 0 583 796 1089
Size: 583 213 293
Fragments arranged by size:
583 293 213
BsiHKAI G_wGCw'C
Cuts at: 0 412 1089
Size: 412 677
BsmI GAATG_Cn'
Cuts at: 0 872 1089
Size: 872 217
Bsp1286I G_dGCh'C
Cuts at: 0 276 412 477 614 909 1089
Size: 276 136 65 137 295 180
Fragments arranged by size:
295 276 180 137 136 65
BspEI T'CCGG_A
Cuts at: 0 443 915 1089
Size: 443 472 174
Fragments arranged by size:
472 443 174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 177 386 1089
Size: 177 209 703
Fragments arranged by size:
703 209 177
BsrBI CCG'CTC
Cuts at: 0 256 1089
Size: 256 833
BsrFI r'CCGG_y
Cuts at: 0 245 1089
Size: 245 844
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 1065 1089
Size: 249 816 24
Fragments arranged by size:
816 249 24
BstYI r'GATC_y
Cuts at: 0 227 742 1089
Size: 227 515 347
Fragments arranged by size:
515 347 227
BtsI GCAGTG_nn'
Cuts at: 0 167 274 956 1089
Size: 167 107 682 133
Fragments arranged by size:
682 167 133 107
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 143 178 1089
Size: 143 35 911
Fragments arranged by size:
911 143 35
DraIII CAC_nnn'GTG
Cuts at: 0 972 1089
Size: 972 117
EaeI y'GGCC_r
Cuts at: 0 251 793 842 1089
Size: 251 542 49 247
Fragments arranged by size:
542 251 247 49
EagI C'GGCC_G
Cuts at: 0 793 1089
Size: 793 296
EarI CTCTTCn'nnn_
Cuts at: 0 508 1089
Size: 508 581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 241 283 376 1089
Size: 95 146 42 93 713
Fragments arranged by size:
713 146 95 93 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 235 899 1089
Size: 235 664 190
Fragments arranged by size:
664 235 190
EcoO109I rG'GnC_Cy
Cuts at: 0 132 199 303 310 500 1078 1089
Size: 132 67 104 7 190 578 11
Fragments arranged by size:
578 190 132 104 67 11 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 119 151 910 942 1089
Size: 119 32 759 32 147
Fragments arranged by size:
759 147 119 32 32
HincII GTy'rAC
Cuts at: 0 826 929 1089
Size: 826 103 160
Fragments arranged by size:
826 160 103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 1089
Size: 530 559
MslI CAynn'nnrTG
Cuts at: 0 97 146 1089
Size: 97 49 943
Fragments arranged by size:
943 97 49
MspA1I CmG'CkG
Cuts at: 0 261 808 1089
Size: 261 547 281
Fragments arranged by size:
547 281 261
PasI CC'CwG_GG
Cuts at: 0 177 1089
Size: 177 912
PpuMI rG'GwC_Cy
Cuts at: 0 1078 1089
Size: 1078 11
PspOMI G'GGCC_C
Cuts at: 0 610 1089
Size: 610 479
PstI C_TGCA'G
Cuts at: 0 281 963 1052 1089
Size: 281 682 89 37
Fragments arranged by size:
682 281 89 37
PvuI CG_AT'CG
Cuts at: 0 583 1089
Size: 583 506
PvuII CAG'CTG
Cuts at: 0 261 808 1089
Size: 261 547 281
Fragments arranged by size:
547 281 261
SbfI CC_TGCA'GG
Cuts at: 0 281 1089
Size: 281 808
SfcI C'TryA_G
Cuts at: 0 277 959 1048 1089
Size: 277 682 89 41
Fragments arranged by size:
682 277 89 41
StuI AGG'CCT
Cuts at: 0 855 1089
Size: 855 234
StyI C'CwwG_G
Cuts at: 0 44 195 1089
Size: 44 151 894
Fragments arranged by size:
894 151 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 540 744 1018 1089
Size: 540 204 274 71
Fragments arranged by size:
540 274 204 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 956 988 1089
Size: 956 32 101
Fragments arranged by size:
956 101 32
Enzymes that do cut:
AarI AcuI AleI AlfI AloI ApaI ApoI AvrII
BanI BanII BbsI BlpI Bme1580I BmrI BpmI BsaI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrFI BstXI BstYI BtsI CspCI
DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I Hin4I
HincII MmeI MslI MspA1I PasI PpuMI PspOMI PstI
PvuI PvuII SbfI SfcI StuI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlwNI ApaLI AscI AseI AsiSI AvaI BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsaXI BsgI BsiWI BsmBI BspHI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DrdI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
RsrII SacI SacII SalI SanDI SapI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI