Restriction map for
pFN21AE4346
(Linear) (Six-base) MAPSORT of: KIEE4346.seq Check: 6139 from: 1 to: 1083
KIEE4346.orf
With 182 enzymes: SgfI *
March 22, 2012 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 555 837 1083
Size: 555 282 246
Fragments arranged by size:
555 282 246
AatII G_ACGT'C
Cuts at: 0 629 1083
Size: 629 454
AccI GT'mk_AC
Cuts at: 0 707 1083
Size: 707 376
Acc65I G'GTAC_C
Cuts at: 0 905 1083
Size: 905 178
AclI AA'CG_TT
Cuts at: 0 790 1083
Size: 790 293
AflIII A'CryG_T
Cuts at: 0 8 1083
Size: 8 1075
AleI CACnn'nnGTG
Cuts at: 0 403 1083
Size: 403 680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 895 1083
Size: 895 188
ApaI G_GGCC'C
Cuts at: 0 508 1083
Size: 508 575
AvaI C'yCGr_G
Cuts at: 0 172 237 1083
Size: 172 65 846
Fragments arranged by size:
846 172 65
BanI G'GyrC_C
Cuts at: 0 133 139 905 973 1083
Size: 133 6 766 68 110
Fragments arranged by size:
766 133 110 68 6
BanII G_rGCy'C
Cuts at: 0 508 589 1083
Size: 508 81 494
Fragments arranged by size:
508 494 81
BbeI G_GCGC'C
Cuts at: 0 137 1083
Size: 137 946
BbvCI CC'TCA_GC
Cuts at: 0 361 1083
Size: 361 722
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 948 982 1083
Size: 948 34 101
Fragments arranged by size:
948 101 34
BglI GCCn_nnn'nGGC
Cuts at: 0 36 199 803 1024 1083
Size: 36 163 604 221 59
Fragments arranged by size:
604 221 163 59 36
Bme1580I G_kGCm'C
Cuts at: 0 244 508 736 1083
Size: 244 264 228 347
Fragments arranged by size:
347 264 244 228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 743 1003 1083
Size: 649 94 260 80
Fragments arranged by size:
649 260 94 80
Bpu10I CC'TnA_GC
Cuts at: 0 361 485 1083
Size: 361 124 598
Fragments arranged by size:
598 361 124
BsaI GGTCTCn'nnnn_
Cuts at: 0 517 1083
Size: 517 566
BsaBI GATnn'nnATC
Cuts at: 0 529 1083
Size: 529 554
BsaHI Gr'CG_yC
Cuts at: 0 134 626 1083
Size: 134 492 457
Fragments arranged by size:
492 457 134
BsaWI w'CCGG_w
Cuts at: 0 260 1083
Size: 260 823
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 689 719 1083
Size: 689 30 364
Fragments arranged by size:
689 364 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 742 925 1083
Size: 265 477 183 158
Fragments arranged by size:
477 265 183 158
BseYI C'CCAG_C
Cuts at: 0 117 501 539 751 1083
Size: 117 384 38 212 332
Fragments arranged by size:
384 332 212 117 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 900 1083
Size: 900 183
Bsp1286I G_dGCh'C
Cuts at: 0 244 508 589 736 1083
Size: 244 264 81 147 347
Fragments arranged by size:
347 264 244 147 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 555 837 926 1083
Size: 555 282 89 157
Fragments arranged by size:
555 282 157 89
BsrFI r'CCGG_y
Cuts at: 0 57 136 713 1083
Size: 57 79 577 370
Fragments arranged by size:
577 370 79 57
BssSI C'ACGA_G
Cuts at: 0 64 631 1083
Size: 64 567 452
Fragments arranged by size:
567 452 64
BstXI CCAn_nnnn'nTGG
Cuts at: 0 291 1083
Size: 291 792
Bsu36I CC'TnA_GG
Cuts at: 0 195 1083
Size: 195 888
DrdI GACnn_nn'nnGTC
Cuts at: 0 543 892 1083
Size: 543 349 191
Fragments arranged by size:
543 349 191
EaeI y'GGCC_r
Cuts at: 0 527 1083
Size: 527 556
EarI CTCTTCn'nnn_
Cuts at: 0 943 1083
Size: 943 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 74 1083
Size: 74 1009
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 743 1003 1083
Size: 649 94 260 80
Fragments arranged by size:
649 260 94 80
EcoNI CCTnn'n_nnAGG
Cuts at: 0 922 1083
Size: 922 161
EcoO109I rG'GnC_Cy
Cuts at: 0 215 505 1025 1083
Size: 215 290 520 58
Fragments arranged by size:
520 290 215 58
HaeII r_GCGC'y
Cuts at: 0 137 194 1083
Size: 137 57 889
Fragments arranged by size:
889 137 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 689 721 1083
Size: 689 32 362
Fragments arranged by size:
689 362 32
HincII GTy'rAC
Cuts at: 0 315 345 1083
Size: 315 30 738
Fragments arranged by size:
738 315 30
KasI G'GCGC_C
Cuts at: 0 133 1083
Size: 133 950
KpnI G_GTAC'C
Cuts at: 0 909 1083
Size: 909 174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 1083
Size: 404 679
MscI TGG'CCA
Cuts at: 0 529 1083
Size: 529 554
MslI CAynn'nnrTG
Cuts at: 0 289 403 739 877 1083
Size: 289 114 336 138 206
Fragments arranged by size:
336 289 206 138 114
MspA1I CmG'CkG
Cuts at: 0 117 420 501 843 912 1083
Size: 117 303 81 342 69 171
Fragments arranged by size:
342 303 171 117 81 69
NaeI GCC'GGC
Cuts at: 0 59 138 1083
Size: 59 79 945
Fragments arranged by size:
945 79 59
NarI GG'CG_CC
Cuts at: 0 134 1083
Size: 134 949
NdeI CA'TA_TG
Cuts at: 0 402 1083
Size: 402 681
NgoMIV G'CCGG_C
Cuts at: 0 57 136 1083
Size: 57 79 947
Fragments arranged by size:
947 79 57
NspI r_CATG'y
Cuts at: 0 810 1083
Size: 810 273
PasI CC'CwG_GG
Cuts at: 0 612 690 1083
Size: 612 78 393
Fragments arranged by size:
612 393 78
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 141 173 1083
Size: 141 32 910
Fragments arranged by size:
910 141 32
PspOMI G'GGCC_C
Cuts at: 0 504 1083
Size: 504 579
PstI C_TGCA'G
Cuts at: 0 848 1083
Size: 848 235
PvuII CAG'CTG
Cuts at: 0 420 501 843 912 1083
Size: 420 81 342 69 171
Fragments arranged by size:
420 342 171 81 69
SfcI C'TryA_G
Cuts at: 0 844 1083
Size: 844 239
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1024 1083
Size: 1024 59
SfoI GGC'GCC
Cuts at: 0 135 1083
Size: 135 948
SgrAI Cr'CCGG_yG
Cuts at: 0 713 1083
Size: 713 370
SmaI CCC'GGG
Cuts at: 0 174 239 1083
Size: 174 65 844
Fragments arranged by size:
844 174 65
SphI G_CATG'C
Cuts at: 0 810 1083
Size: 810 273
StyI C'CwwG_G
Cuts at: 0 923 1020 1083
Size: 923 97 63
Fragments arranged by size:
923 97 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 442 682 841 961 1083
Size: 442 240 159 120 122
Fragments arranged by size:
442 240 159 122 120
TatI w'GTAC_w
Cuts at: 0 444 1083
Size: 444 639
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 957 989 1083
Size: 957 32 94
Fragments arranged by size:
957 94 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 718 1083
Size: 718 365
XmaI C'CCGG_G
Cuts at: 0 172 237 1083
Size: 172 65 846
Fragments arranged by size:
846 172 65
ZraI GAC'GTC
Cuts at: 0 627 1083
Size: 627 456
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AflIII AleI AlfI
ApaI AvaI BanI BanII BbeI BbvCI BcgI BglI
Bme1580I BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsmBI Bsp1286I BspMI BsrFI BssSI BstXI
Bsu36I DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PasI PpiI
PspOMI PstI PvuII SfcI SfiI SfoI SgrAI SmaI
SphI StyI TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AcuI AfeI AflII AgeI AhdI AloI AlwNI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BpuEI BsaAI BsgI BsiEI BsiHKAI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII EagI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI MfeI MluI NcoI NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SgfI SmlI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmnI