Restriction map for
pFN21AE4341
(Linear) (Six-base) MAPSORT of: KIEE4341.seq Check: 159 from: 1 to: 1062
KIEE4341.orf
With 182 enzymes: SgfI *
July 3, 2012 18:59 ..
AccI GT'mk_AC
Cuts at: 0 458 1062
Size: 458 604
AclI AA'CG_TT
Cuts at: 0 447 1062
Size: 447 615
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 793 964 1062
Size: 176 617 171 98
Fragments arranged by size:
617 176 171 98
AleI CACnn'nnGTG
Cuts at: 0 796 991 1062
Size: 796 195 71
Fragments arranged by size:
796 195 71
ApoI r'AATT_y
Cuts at: 0 430 1016 1062
Size: 430 586 46
Fragments arranged by size:
586 430 46
AvaI C'yCGr_G
Cuts at: 0 92 1062
Size: 92 970
BanI G'GyrC_C
Cuts at: 0 790 985 1027 1062
Size: 790 195 42 35
Fragments arranged by size:
790 195 42 35
BanII G_rGCy'C
Cuts at: 0 1061 1062
Size: 1061 1
Bme1580I G_kGCm'C
Cuts at: 0 291 793 988 1062
Size: 291 502 195 74
Fragments arranged by size:
502 291 195 74
BmrI ACTGGGnnnn_n'
Cuts at: 0 765 1062
Size: 765 297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 878 1062
Size: 180 698 184
Fragments arranged by size:
698 184 180
BsaI GGTCTCn'nnnn_
Cuts at: 0 536 1062
Size: 536 526
BseYI C'CCAG_C
Cuts at: 0 27 1062
Size: 27 1035
BsiHKAI G_wGCw'C
Cuts at: 0 952 1061 1062
Size: 952 109 1
Fragments arranged by size:
952 109 1
BsmI GAATG_Cn'
Cuts at: 0 90 1062
Size: 90 972
Bsp1286I G_dGCh'C
Cuts at: 0 291 793 952 988 1061 1062
Size: 291 502 159 36 73 1
Fragments arranged by size:
502 291 159 73 36 1
BssSI C'ACGA_G
Cuts at: 0 10 1062
Size: 10 1052
BstYI r'GATC_y
Cuts at: 0 264 591 1062
Size: 264 327 471
Fragments arranged by size:
471 327 264
BstZ17I GTA'TAC
Cuts at: 0 459 1062
Size: 459 603
Bsu36I CC'TnA_GG
Cuts at: 0 884 1062
Size: 884 178
BtgI C'CryG_G
Cuts at: 0 1008 1062
Size: 1008 54
DraI TTT'AAA
Cuts at: 0 429 1062
Size: 429 633
EaeI y'GGCC_r
Cuts at: 0 644 1062
Size: 644 418
EcoICRI GAG'CTC
Cuts at: 0 1059 1062
Size: 1059 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 180 793 878 964 1062
Size: 176 4 613 85 86 98
Fragments arranged by size:
613 176 98 86 85 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 133 824 1062
Size: 133 691 238
Fragments arranged by size:
691 238 133
EcoO109I rG'GnC_Cy
Cuts at: 0 888 1062
Size: 888 174
HaeII r_GCGC'y
Cuts at: 0 19 1062
Size: 19 1043
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 576 608 801 833 1062
Size: 576 32 193 32 229
Fragments arranged by size:
576 229 193 32 32
MscI TGG'CCA
Cuts at: 0 646 1062
Size: 646 416
MslI CAynn'nnrTG
Cuts at: 0 796 991 1062
Size: 796 195 71
Fragments arranged by size:
796 195 71
NcoI C'CATG_G
Cuts at: 0 1008 1062
Size: 1008 54
NspI r_CATG'y
Cuts at: 0 222 1062
Size: 222 840
PasI CC'CwG_GG
Cuts at: 0 941 1062
Size: 941 121
PfoI T'CCnGG_A
Cuts at: 0 894 1062
Size: 894 168
PpuMI rG'GwC_Cy
Cuts at: 0 888 1062
Size: 888 174
SacI G_AGCT'C
Cuts at: 0 1061 1062
Size: 1061 1
ScaI AGT'ACT
Cuts at: 0 908 1062
Size: 908 154
SfcI C'TryA_G
Cuts at: 0 618 1062
Size: 618 444
SmlI C'TyrA_G
Cuts at: 0 92 1062
Size: 92 970
SphI G_CATG'C
Cuts at: 0 222 1062
Size: 222 840
StyI C'CwwG_G
Cuts at: 0 283 625 1008 1062
Size: 283 342 383 54
Fragments arranged by size:
383 342 283 54
SwaI ATTT'AAAT
Cuts at: 0 429 1062
Size: 429 633
TatI w'GTAC_w
Cuts at: 0 906 1049 1062
Size: 906 143 13
Fragments arranged by size:
906 143 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 394 426 1062
Size: 394 32 636
Fragments arranged by size:
636 394 32
XhoI C'TCGA_G
Cuts at: 0 92 1062
Size: 92 970
XmnI GAAnn'nnTTC
Cuts at: 0 908 1062
Size: 908 154
Enzymes that do cut:
AccI AclI AcuI AleI ApoI AvaI BanI BanII
Bme1580I BmrI BpmI BsaI BseYI BsiHKAI BsmI Bsp1286I
BssSI BstYI BstZ17I Bsu36I BtgI DraI EaeI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I MscI MslI NcoI
NspI PasI PfoI PpuMI SacI ScaI SfcI SmlI
SphI StyI SwaI TatI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EarI
EciI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI TaqII
Tth111I XbaI XcmI XmaI ZraI