Restriction map for
pFN21AE4338
(Linear) (Six-base) MAPSORT of: KIEE4338.seq Check: 7026 from: 1 to: 1050
KIEE4338.orf
With 182 enzymes: SgfI *
March 1, 2012 16:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 508 1050
Size: 508 542
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1050
Size: 1007 43
AhdI GACnn_n'nnGTC
Cuts at: 0 376 1050
Size: 376 674
AleI CACnn'nnGTG
Cuts at: 0 403 1050
Size: 403 647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 715 1050
Size: 715 335
AlwNI CAG_nnn'CTG
Cuts at: 0 960 1050
Size: 960 90
ApaI G_GGCC'C
Cuts at: 0 464 1050
Size: 464 586
ApaLI G'TGCA_C
Cuts at: 0 331 690 1050
Size: 331 359 360
Fragments arranged by size:
360 359 331
ApoI r'AATT_y
Cuts at: 0 341 1050
Size: 341 709
AvaI C'yCGr_G
Cuts at: 0 536 1050
Size: 536 514
BanII G_rGCy'C
Cuts at: 0 464 1050
Size: 464 586
Bme1580I G_kGCm'C
Cuts at: 0 335 464 694 1050
Size: 335 129 230 356
Fragments arranged by size:
356 335 230 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 982 991 1050
Size: 74 908 9 59
Fragments arranged by size:
908 74 59 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 283 551 1050
Size: 283 268 499
Fragments arranged by size:
499 283 268
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 72 102 1050
Size: 72 30 948
Fragments arranged by size:
948 72 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 802 958 990 1050
Size: 142 660 156 32 60
Fragments arranged by size:
660 156 142 60 32
BseYI C'CCAG_C
Cuts at: 0 274 463 999 1032 1050
Size: 274 189 536 33 18
Fragments arranged by size:
536 274 189 33 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 567 1010 1050
Size: 516 51 443 40
Fragments arranged by size:
516 443 51 40
BsiHKAI G_wGCw'C
Cuts at: 0 335 694 1050
Size: 335 359 356
Fragments arranged by size:
359 356 335
BsmBI CGTCTCn'nnnn_
Cuts at: 0 404 1050
Size: 404 646
Bsp1286I G_dGCh'C
Cuts at: 0 335 464 694 1050
Size: 335 129 230 356
Fragments arranged by size:
356 335 230 129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 508 1050
Size: 508 542
BsrBI CCG'CTC
Cuts at: 0 70 1050
Size: 70 980
BsrDI GCAATG_nn'
Cuts at: 0 615 1050
Size: 615 435
BsrFI r'CCGG_y
Cuts at: 0 737 1050
Size: 737 313
BssSI C'ACGA_G
Cuts at: 0 433 1050
Size: 433 617
BstAPI GCAn_nnn'nTGC
Cuts at: 0 192 473 1050
Size: 192 281 577
Fragments arranged by size:
577 281 192
BstYI r'GATC_y
Cuts at: 0 890 1050
Size: 890 160
BtgI C'CryG_G
Cuts at: 0 53 704 1050
Size: 53 651 346
Fragments arranged by size:
651 346 53
BtsI GCAGTG_nn'
Cuts at: 0 579 1027 1050
Size: 579 448 23
Fragments arranged by size:
579 448 23
EaeI y'GGCC_r
Cuts at: 0 593 1050
Size: 593 457
EarI CTCTTCn'nnn_
Cuts at: 0 203 983 1037 1050
Size: 203 780 54 13
Fragments arranged by size:
780 203 54 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 982 991 1007 1050
Size: 74 908 9 16 43
Fragments arranged by size:
908 74 43 16 9
EcoO109I rG'GnC_Cy
Cuts at: 0 909 966 1050
Size: 909 57 84
Fragments arranged by size:
909 84 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 876 1050
Size: 876 174
FspI TGC'GCA
Cuts at: 0 196 1050
Size: 196 854
HaeII r_GCGC'y
Cuts at: 0 1000 1050
Size: 1000 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 72 104 362 394 1050
Size: 72 32 258 32 656
Fragments arranged by size:
656 258 72 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 291 1050
Size: 97 194 759
Fragments arranged by size:
759 194 97
MscI TGG'CCA
Cuts at: 0 595 1050
Size: 595 455
MslI CAynn'nnrTG
Cuts at: 0 328 403 589 1050
Size: 328 75 186 461
Fragments arranged by size:
461 328 186 75
MspA1I CmG'CkG
Cuts at: 0 6 564 1024 1050
Size: 6 558 460 26
Fragments arranged by size:
558 460 26 6
NaeI GCC'GGC
Cuts at: 0 739 1050
Size: 739 311
NgoMIV G'CCGG_C
Cuts at: 0 737 1050
Size: 737 313
NspI r_CATG'y
Cuts at: 0 471 1050
Size: 471 579
PflMI CCAn_nnn'nTGG
Cuts at: 0 837 960 1050
Size: 837 123 90
Fragments arranged by size:
837 123 90
PpuMI rG'GwC_Cy
Cuts at: 0 909 966 1050
Size: 909 57 84
Fragments arranged by size:
909 84 57
PspOMI G'GGCC_C
Cuts at: 0 460 1050
Size: 460 590
PvuII CAG'CTG
Cuts at: 0 564 1050
Size: 564 486
SmaI CCC'GGG
Cuts at: 0 538 1050
Size: 538 512
StuI AGG'CCT
Cuts at: 0 674 1050
Size: 674 376
StyI C'CwwG_G
Cuts at: 0 782 1050
Size: 782 268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 803 1050
Size: 803 247
TatI w'GTAC_w
Cuts at: 0 470 494 1050
Size: 470 24 556
Fragments arranged by size:
556 470 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 532 564 606 638 1050
Size: 532 32 42 32 412
Fragments arranged by size:
532 412 42 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 741 1050
Size: 741 309
XmaI C'CCGG_G
Cuts at: 0 536 1050
Size: 536 514
Enzymes that do cut:
AarI AcuI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanII Bme1580I BpmI BsaI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstYI BtgI BtsI EaeI EarI
Eco57MI EcoO109I FalI FspI HaeII Hin4I MmeI MscI
MslI MspA1I NaeI NgoMIV NspI PflMI PpuMI PspOMI
PvuII SmaI StuI StyI TaqII TatI TstI XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AloI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI
BspHI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmnI ZraI