Restriction map for
pFN21AE4336
(Linear) (Six-base) MAPSORT of: KIEE4336.seq Check: 637 from: 1 to: 1041
KIEE4336.orf
With 182 enzymes: SgfI *
March 22, 2012 17:46 ..
AccI GT'mk_AC
Cuts at: 0 101 1041
Size: 101 940
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1041
Size: 829 212
AfeI AGC'GCT
Cuts at: 0 64 1041
Size: 64 977
AleI CACnn'nnGTG
Cuts at: 0 136 1041
Size: 136 905
AlwNI CAG_nnn'CTG
Cuts at: 0 87 441 1041
Size: 87 354 600
Fragments arranged by size:
600 354 87
AvaI C'yCGr_G
Cuts at: 0 276 1041
Size: 276 765
BanII G_rGCy'C
Cuts at: 0 803 947 1041
Size: 803 144 94
Fragments arranged by size:
803 144 94
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 124 158 1041
Size: 124 34 883
Fragments arranged by size:
883 124 34
BglI GCCn_nnn'nGGC
Cuts at: 0 935 973 1041
Size: 935 38 68
Fragments arranged by size:
935 68 38
Bme1580I G_kGCm'C
Cuts at: 0 969 1041
Size: 969 72
BmgBI CAC'GTC
Cuts at: 0 138 528 1041
Size: 138 390 513
Fragments arranged by size:
513 390 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 554 966 1041
Size: 554 412 75
Fragments arranged by size:
554 412 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 436 759 817 991 1041
Size: 219 217 323 58 174 50
Fragments arranged by size:
323 219 217 174 58 50
Bpu10I CC'TnA_GC
Cuts at: 0 226 1041
Size: 226 815
BsaI GGTCTCn'nnnn_
Cuts at: 0 566 1041
Size: 566 475
BsaHI Gr'CG_yC
Cuts at: 0 617 1041
Size: 617 424
BsaWI w'CCGG_w
Cuts at: 0 306 1041
Size: 306 735
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 11 41 1041
Size: 11 30 1000
Fragments arranged by size:
1000 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 517 1041
Size: 517 524
BseYI C'CCAG_C
Cuts at: 0 556 1041
Size: 556 485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 1041
Size: 525 516
BsmI GAATG_Cn'
Cuts at: 0 819 1028 1041
Size: 819 209 13
Fragments arranged by size:
819 209 13
Bsp1286I G_dGCh'C
Cuts at: 0 803 947 969 1041
Size: 803 144 22 72
Fragments arranged by size:
803 144 72 22
BspEI T'CCGG_A
Cuts at: 0 306 1041
Size: 306 735
BspHI T'CATG_A
Cuts at: 0 605 1041
Size: 605 436
BsrFI r'CCGG_y
Cuts at: 0 145 1041
Size: 145 896
BstXI CCAn_nnnn'nTGG
Cuts at: 0 138 363 925 1041
Size: 138 225 562 116
Fragments arranged by size:
562 225 138 116
BtgI C'CryG_G
Cuts at: 0 930 1041
Size: 930 111
DrdI GACnn_nn'nnGTC
Cuts at: 0 97 532 1041
Size: 97 435 509
Fragments arranged by size:
509 435 97
EaeI y'GGCC_r
Cuts at: 0 847 927 1041
Size: 847 80 114
Fragments arranged by size:
847 114 80
EarI CTCTTCn'nnn_
Cuts at: 0 295 1041
Size: 295 746
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 208 1041
Size: 208 833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 436 759 817 829 991 1041
Size: 219 217 323 58 12 162 50
Fragments arranged by size:
323 219 217 162 58 50 12
EcoO109I rG'GnC_Cy
Cuts at: 0 695 1041
Size: 695 346
HaeII r_GCGC'y
Cuts at: 0 66 565 1041
Size: 66 499 476
Fragments arranged by size:
499 476 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 11 43 693 725 1041
Size: 11 32 650 32 316
Fragments arranged by size:
650 316 32 32 11
HincII GTy'rAC
Cuts at: 0 455 537 1041
Size: 455 82 504
Fragments arranged by size:
504 455 82
HpaI GTT'AAC
Cuts at: 0 455 1041
Size: 455 586
MscI TGG'CCA
Cuts at: 0 929 1041
Size: 929 112
MslI CAynn'nnrTG
Cuts at: 0 136 610 923 1041
Size: 136 474 313 118
Fragments arranged by size:
474 313 136 118
MspA1I CmG'CkG
Cuts at: 0 483 1041
Size: 483 558
NaeI GCC'GGC
Cuts at: 0 147 1041
Size: 147 894
NcoI C'CATG_G
Cuts at: 0 930 1041
Size: 930 111
NgoMIV G'CCGG_C
Cuts at: 0 145 1041
Size: 145 896
PasI CC'CwG_GG
Cuts at: 0 691 1041
Size: 691 350
PflMI CCAn_nnn'nTGG
Cuts at: 0 956 1041
Size: 956 85
PfoI T'CCnGG_A
Cuts at: 0 316 758 998 1041
Size: 316 442 240 43
Fragments arranged by size:
442 316 240 43
PpuMI rG'GwC_Cy
Cuts at: 0 695 1041
Size: 695 346
PstI C_TGCA'G
Cuts at: 0 83 587 793 1041
Size: 83 504 206 248
Fragments arranged by size:
504 248 206 83
RsrII CG'GwC_CG
Cuts at: 0 108 1041
Size: 108 933
SanDI GG'GwC_CC
Cuts at: 0 695 1041
Size: 695 346
SfcI C'TryA_G
Cuts at: 0 79 433 583 789 1041
Size: 79 354 150 206 252
Fragments arranged by size:
354 252 206 150 79
StyI C'CwwG_G
Cuts at: 0 622 930 1041
Size: 622 308 111
Fragments arranged by size:
622 308 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 661 1041
Size: 661 380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 135 1041
Size: 135 906
XmnI GAAnn'nnTTC
Cuts at: 0 302 475 634 884 1041
Size: 302 173 159 250 157
Fragments arranged by size:
302 250 173 159 157
Enzymes that do cut:
AccI AcuI AfeI AleI AlwNI AvaI BanII BcgI
BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspEI
BspHI BsrFI BstXI BtgI DrdI EaeI EarI EciI
Eco57MI EcoO109I HaeII Hin4I HincII HpaI MscI MslI
MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI PpuMI
PstI RsrII SanDI SfcI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI BmtI BplI BpuEI BsaAI
BsaBI BsiEI BsiHKAI BsiWI BsmBI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII KasI KpnI MfeI MluI MmeI NarI NdeI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmaI ZraI