(Linear) (Six-base) MAPSORT of: KIEE4335.seq  Check: 1237  from: 1  to: 1035



KIEE4335.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:46  ..

Acc65I G'GTAC_C

Cuts at:      0     718    1035 
   Size:        718     317
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     275    1035 
   Size:        275     760
 
ApoI r'AATT_y

Cuts at:      0     115     822    1035 
   Size:        115     707     213

  Fragments arranged by size: 

                 707     213     115
 
AvaI C'yCGr_G

Cuts at:      0      98     311    1035 
   Size:         98     213     724

  Fragments arranged by size: 

                 724     213      98
 
BanI G'GyrC_C

Cuts at:      0     718    1035 
   Size:        718     317
 
BanII G_rGCy'C

Cuts at:      0     991    1035 
   Size:        991      44
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     416    1035 
   Size:        416     619
 
BfrBI ATG'CAT

Cuts at:      0     451    1035 
   Size:        451     584
 
BglI GCCn_nnn'nGGC

Cuts at:      0     388    1035 
   Size:        388     647
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     394    1035 
   Size:        373      21     641

  Fragments arranged by size: 

                 641     373      21
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     285     498    1035 
   Size:        285     213     537

  Fragments arranged by size: 

                 537     285     213
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     954     984    1035 
   Size:        954      30      51

  Fragments arranged by size: 

                 954      51      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     959     962     965     981    1035 
   Size:        959       3       3      16      54

  Fragments arranged by size: 

                 959      54      16       3       3
 
Bsp1286I G_dGCh'C

Cuts at:      0     991    1035 
   Size:        991      44
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     267    1035 
   Size:        267     768
 
BsrDI GCAATG_nn'

Cuts at:      0     900    1035 
   Size:        900     135
 
BsrGI T'GTAC_A

Cuts at:      0     498    1035 
   Size:        498     537
 
BstYI r'GATC_y

Cuts at:      0     771    1035 
   Size:        771     264
 
DraI TTT'AAA

Cuts at:      0      35    1035 
   Size:         35    1000
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     625    1035 
   Size:        625     410
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     612    1035 
   Size:        612     423
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     394    1035 
   Size:        373      21     641

  Fragments arranged by size: 

                 641     373      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     741    1035 
   Size:        741     294
 
EcoRI G'AATT_C

Cuts at:      0     115    1035 
   Size:        115     920
 
EcoRV GAT'ATC

Cuts at:      0     749    1035 
   Size:        749     286
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     102     655    1035 
   Size:        102     553     380

  Fragments arranged by size: 

                 553     380     102
 
HaeII r_GCGC'y

Cuts at:      0    1012    1035 
   Size:       1012      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     826     858    1035 
   Size:        826      32     177

  Fragments arranged by size: 

                 826     177      32
 
HincII GTy'rAC

Cuts at:      0     873    1035 
   Size:        873     162
 
HindIII A'AGCT_T

Cuts at:      0      84    1035 
   Size:         84     951
 
HpaI GTT'AAC

Cuts at:      0     873    1035 
   Size:        873     162
 
KpnI G_GTAC'C

Cuts at:      0     722    1035 
   Size:        722     313
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     619    1035 
   Size:        131     488     416

  Fragments arranged by size: 

                 488     416     131
 
MslI CAynn'nnrTG

Cuts at:      0     257     338    1035 
   Size:        257      81     697

  Fragments arranged by size: 

                 697     257      81
 
NsiI A_TGCA'T

Cuts at:      0     453    1035 
   Size:        453     582
 
PfoI T'CCnGG_A

Cuts at:      0      89     119    1035 
   Size:         89      30     916

  Fragments arranged by size: 

                 916      89      30
 
TatI w'GTAC_w

Cuts at:      0     165     498    1035 
   Size:        165     333     537

  Fragments arranged by size: 

                 537     333     165
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     954     986    1035 
   Size:        954      32      49

  Fragments arranged by size: 

                 954      49      32
 
XbaI T'CTAG_A

Cuts at:      0     600    1035 
   Size:        600     435
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     369    1035 
   Size:        369     666
 

 Enzymes that do cut:

   Acc65I     AlfI     ApoI     AvaI     BanI    BanII     BbsI    BfrBI
     BglI     BpmI     BsaI    BsaXI    BseRI Bsp1286I    BspMI    BsrDI
    BsrGI    BstYI     DraI     DrdI     EciI  Eco57MI    EcoNI    EcoRI
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MmeI     MslI     NsiI     PfoI     TatI     TstI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     EaeI     EagI     EarI  EcoICRI EcoO109I     FseI     FspI    FspAI
     KasI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I
     XhoI     XmaI     XmnI     ZraI