Restriction map for
pFN21AE4334
(Linear) (Six-base) MAPSORT of: KIEE4334.seq Check: 2975 from: 1 to: 1032
KIEE4334.orf
With 182 enzymes: SgfI *
March 22, 2012 17:45 ..
AflIII A'CryG_T
Cuts at: 0 280 532 1032
Size: 280 252 500
Fragments arranged by size:
500 280 252
AhdI GACnn_n'nnGTC
Cuts at: 0 831 1032
Size: 831 201
AleI CACnn'nnGTG
Cuts at: 0 535 1032
Size: 535 497
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 455 487 1032
Size: 455 32 545
Fragments arranged by size:
545 455 32
AlwNI CAG_nnn'CTG
Cuts at: 0 132 595 650 1032
Size: 132 463 55 382
Fragments arranged by size:
463 382 132 55
ApaLI G'TGCA_C
Cuts at: 0 428 747 1032
Size: 428 319 285
Fragments arranged by size:
428 319 285
BanI G'GyrC_C
Cuts at: 0 14 250 1032
Size: 14 236 782
Fragments arranged by size:
782 236 14
BbeI G_GCGC'C
Cuts at: 0 254 1032
Size: 254 778
BbsI GAAGACnn'nnnn_
Cuts at: 0 968 995 1032
Size: 968 27 37
Fragments arranged by size:
968 37 27
BglI GCCn_nnn'nGGC
Cuts at: 0 27 680 1032
Size: 27 653 352
Fragments arranged by size:
653 352 27
BglII A'GATC_T
Cuts at: 0 685 1032
Size: 685 347
BlpI GC'TnA_GC
Cuts at: 0 181 1032
Size: 181 851
Bme1580I G_kGCm'C
Cuts at: 0 81 432 751 823 1032
Size: 81 351 319 72 209
Fragments arranged by size:
351 319 209 81 72
BmgBI CAC'GTC
Cuts at: 0 207 1032
Size: 207 825
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1032
Size: 627 405
BsaHI Gr'CG_yC
Cuts at: 0 251 1032
Size: 251 781
BsaWI w'CCGG_w
Cuts at: 0 937 1032
Size: 937 95
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 214 897 991 1032
Size: 172 42 683 94 41
Fragments arranged by size:
683 172 94 42 41
BseYI C'CCAG_C
Cuts at: 0 85 678 1032
Size: 85 593 354
Fragments arranged by size:
593 354 85
BsiEI CG_ry'CG
Cuts at: 0 120 177 1032
Size: 120 57 855
Fragments arranged by size:
855 120 57
BsiHKAI G_wGCw'C
Cuts at: 0 187 432 751 1032
Size: 187 245 319 281
Fragments arranged by size:
319 281 245 187
Bsp1286I G_dGCh'C
Cuts at: 0 81 187 432 751 823 1032
Size: 81 106 245 319 72 209
Fragments arranged by size:
319 245 209 106 81 72
BspEI T'CCGG_A
Cuts at: 0 937 1032
Size: 937 95
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 679 1032
Size: 679 353
BsrDI GCAATG_nn'
Cuts at: 0 426 1032
Size: 426 606
BsrFI r'CCGG_y
Cuts at: 0 172 176 794 1032
Size: 172 4 618 238
Fragments arranged by size:
618 238 172 4
BsrGI T'GTAC_A
Cuts at: 0 134 1032
Size: 134 898
BssSI C'ACGA_G
Cuts at: 0 189 1032
Size: 189 843
BstXI CCAn_nnnn'nTGG
Cuts at: 0 25 537 751 1032
Size: 25 512 214 281
Fragments arranged by size:
512 281 214 25
BstYI r'GATC_y
Cuts at: 0 685 940 1032
Size: 685 255 92
Fragments arranged by size:
685 255 92
Bsu36I CC'TnA_GG
Cuts at: 0 909 1032
Size: 909 123
BtsI GCAGTG_nn'
Cuts at: 0 81 1032
Size: 81 951
DraIII CAC_nnn'GTG
Cuts at: 0 191 1032
Size: 191 841
EaeI y'GGCC_r
Cuts at: 0 117 174 1032
Size: 117 57 858
Fragments arranged by size:
858 117 57
EagI C'GGCC_G
Cuts at: 0 117 174 1032
Size: 117 57 858
Fragments arranged by size:
858 117 57
EarI CTCTTCn'nnn_
Cuts at: 0 229 968 1032
Size: 229 739 64
Fragments arranged by size:
739 229 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1032
Size: 627 405
EcoNI CCTnn'n_nnAGG
Cuts at: 0 661 1032
Size: 661 371
EcoO109I rG'GnC_Cy
Cuts at: 0 129 1032
Size: 129 903
EcoRV GAT'ATC
Cuts at: 0 741 1032
Size: 741 291
HaeII r_GCGC'y
Cuts at: 0 254 1032
Size: 254 778
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 561 593 817 849 1032
Size: 561 32 224 32 183
Fragments arranged by size:
561 224 183 32 32
KasI G'GCGC_C
Cuts at: 0 250 1032
Size: 250 782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 815 1032
Size: 392 423 217
Fragments arranged by size:
423 392 217
MslI CAynn'nnrTG
Cuts at: 0 535 1032
Size: 535 497
MspA1I CmG'CkG
Cuts at: 0 122 344 1032
Size: 122 222 688
Fragments arranged by size:
688 222 122
NaeI GCC'GGC
Cuts at: 0 178 1032
Size: 178 854
NarI GG'CG_CC
Cuts at: 0 251 1032
Size: 251 781
NgoMIV G'CCGG_C
Cuts at: 0 176 1032
Size: 176 856
NspI r_CATG'y
Cuts at: 0 536 1032
Size: 536 496
PciI A'CATG_T
Cuts at: 0 532 1032
Size: 532 500
PflMI CCAn_nnn'nTGG
Cuts at: 0 63 1032
Size: 63 969
PpuMI rG'GwC_Cy
Cuts at: 0 129 1032
Size: 129 903
PstI C_TGCA'G
Cuts at: 0 169 1032
Size: 169 863
SfcI C'TryA_G
Cuts at: 0 165 999 1032
Size: 165 834 33
Fragments arranged by size:
834 165 33
SfoI GGC'GCC
Cuts at: 0 252 1032
Size: 252 780
StyI C'CwwG_G
Cuts at: 0 480 1032
Size: 480 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 157 211 1032
Size: 157 54 821
Fragments arranged by size:
821 157 54
TatI w'GTAC_w
Cuts at: 0 134 1032
Size: 134 898
XbaI T'CTAG_A
Cuts at: 0 1023 1032
Size: 1023 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 537 751 1032
Size: 537 214 281
Fragments arranged by size:
537 281 214
Enzymes that do cut:
AflIII AhdI AleI AloI AlwNI ApaLI BanI BbeI
BbsI BglI BglII BlpI Bme1580I BmgBI BpmI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtsI
DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRV
HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PpuMI PstI SfcI SfoI
StyI TaqII TatI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AlfI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbvCI BcgI BciVI BclI
BfrBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaXI BsgI BsiWI BsmI BsmBI BspHI BsrBI
BssHII BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI
CspCI DraI DrdI EciI EcoICRI EcoRI FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MscI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XhoI XmaI XmnI ZraI