Restriction map for
pFN21AE4333
(Linear) (Six-base) MAPSORT of: KIEE4333.seq Check: 9367 from: 1 to: 1029
KIEE4333.orf
With 182 enzymes: SgfI *
March 22, 2012 17:45 ..
Acc65I G'GTAC_C
Cuts at: 0 749 1029
Size: 749 280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 918 1029
Size: 370 548 111
Fragments arranged by size:
548 370 111
AflIII A'CryG_T
Cuts at: 0 598 1029
Size: 598 431
ApaI G_GGCC'C
Cuts at: 0 306 721 792 1029
Size: 306 415 71 237
Fragments arranged by size:
415 306 237 71
AvaI C'yCGr_G
Cuts at: 0 571 1029
Size: 571 458
BamHI G'GATC_C
Cuts at: 0 839 1029
Size: 839 190
BanI G'GyrC_C
Cuts at: 0 576 749 876 1029
Size: 576 173 127 153
Fragments arranged by size:
576 173 153 127
BanII G_rGCy'C
Cuts at: 0 306 391 721 792 1029
Size: 306 85 330 71 237
Fragments arranged by size:
330 306 237 85 71
BbvCI CC'TCA_GC
Cuts at: 0 961 1029
Size: 961 68
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 135 169 1029
Size: 135 34 860
Fragments arranged by size:
860 135 34
BclI T'GATC_A
Cuts at: 0 584 1029
Size: 584 445
BglI GCCn_nnn'nGGC
Cuts at: 0 787 1029
Size: 787 242
BlpI GC'TnA_GC
Cuts at: 0 1018 1029
Size: 1018 11
Bme1580I G_kGCm'C
Cuts at: 0 306 721 792 881 1029
Size: 306 415 71 89 148
Fragments arranged by size:
415 306 148 89 71
BmgBI CAC'GTC
Cuts at: 0 599 1029
Size: 599 430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 807 847 1029
Size: 364 443 40 182
Fragments arranged by size:
443 364 182 40
Bpu10I CC'TnA_GC
Cuts at: 0 961 1029
Size: 961 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 748 1029
Size: 748 281
BsaHI Gr'CG_yC
Cuts at: 0 197 332 550 623 695 1029
Size: 197 135 218 73 72 334
Fragments arranged by size:
334 218 197 135 73 72
BsaWI w'CCGG_w
Cuts at: 0 71 1029
Size: 71 958
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 307 337 1029
Size: 307 30 692
Fragments arranged by size:
692 307 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 649 994 1029
Size: 400 249 345 35
Fragments arranged by size:
400 345 249 35
BseYI C'CCAG_C
Cuts at: 0 33 262 297 370 515 770 896 945
Size: 33 229 35 73 145 255 126 49
Cuts at: 945 1029
Size: 84
Fragments arranged by size:
255 229 145 126 84 73 49 35
33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 486 1029
Size: 170 316 543
Fragments arranged by size:
543 316 170
BsiEI CG_ry'CG
Cuts at: 0 76 1029
Size: 76 953
BsiHKAI G_wGCw'C
Cuts at: 0 272 1028 1029
Size: 272 756 1
Fragments arranged by size:
756 272 1
BsmBI CGTCTCn'nnnn_
Cuts at: 0 589 1029
Size: 589 440
Bsp1286I G_dGCh'C
Cuts at: 0 272 306 391 721 792 881 1028 1029
Size: 272 34 85 330 71 89 147 1
Fragments arranged by size:
330 272 147 89 85 71 34 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 165 1029
Size: 165 864
BsrBI CCG'CTC
Cuts at: 0 88 1029
Size: 88 941
BsrDI GCAATG_nn'
Cuts at: 0 401 1029
Size: 401 628
BsrFI r'CCGG_y
Cuts at: 0 157 1029
Size: 157 872
BsrGI T'GTAC_A
Cuts at: 0 611 809 1029
Size: 611 198 220
Fragments arranged by size:
611 220 198
BssSI C'ACGA_G
Cuts at: 0 220 869 1029
Size: 220 649 160
Fragments arranged by size:
649 220 160
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 1029
Size: 237 792
BstXI CCAn_nnnn'nTGG
Cuts at: 0 208 1029
Size: 208 821
BstYI r'GATC_y
Cuts at: 0 839 1029
Size: 839 190
Bsu36I CC'TnA_GG
Cuts at: 0 632 1029
Size: 632 397
BtgI C'CryG_G
Cuts at: 0 1012 1029
Size: 1012 17
BtsI GCAGTG_nn'
Cuts at: 0 153 238 252 498 559 1029
Size: 153 85 14 246 61 470
Fragments arranged by size:
470 246 153 85 61 14
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 897 932 1029
Size: 897 35 97
Fragments arranged by size:
897 97 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 337 1029
Size: 337 692
EaeI y'GGCC_r
Cuts at: 0 44 1029
Size: 44 985
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 370 807 847 918 1029
Size: 364 6 437 40 71 111
Fragments arranged by size:
437 364 111 71 40 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 382 1029
Size: 382 647
EcoO109I rG'GnC_Cy
Cuts at: 0 15 22 302 717 788 1029
Size: 15 7 280 415 71 241
Fragments arranged by size:
415 280 241 71 15 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 1029
Size: 307 32 690
Fragments arranged by size:
690 307 32
KpnI G_GTAC'C
Cuts at: 0 753 1029
Size: 753 276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 1029
Size: 438 591
MscI TGG'CCA
Cuts at: 0 46 1029
Size: 46 983
MslI CAynn'nnrTG
Cuts at: 0 206 1029
Size: 206 823
MspA1I CmG'CkG
Cuts at: 0 33 247 1029
Size: 33 214 782
Fragments arranged by size:
782 214 33
NcoI C'CATG_G
Cuts at: 0 1012 1029
Size: 1012 17
PasI CC'CwG_GG
Cuts at: 0 393 951 1029
Size: 393 558 78
Fragments arranged by size:
558 393 78
PfoI T'CCnGG_A
Cuts at: 0 380 835 1029
Size: 380 455 194
Fragments arranged by size:
455 380 194
PpuMI rG'GwC_Cy
Cuts at: 0 15 1029
Size: 15 1014
PspOMI G'GGCC_C
Cuts at: 0 302 717 788 1029
Size: 302 415 71 241
Fragments arranged by size:
415 302 241 71
PspXI vC'TCGA_Gb
Cuts at: 0 571 1029
Size: 571 458
PstI C_TGCA'G
Cuts at: 0 471 566 1029
Size: 471 95 463
Fragments arranged by size:
471 463 95
PvuII CAG'CTG
Cuts at: 0 33 247 1029
Size: 33 214 782
Fragments arranged by size:
782 214 33
SfcI C'TryA_G
Cuts at: 0 467 562 1029
Size: 467 95 467
Fragments arranged by size:
467 467 95
SmlI C'TyrA_G
Cuts at: 0 571 1029
Size: 571 458
StuI AGG'CCT
Cuts at: 0 801 1029
Size: 801 228
StyI C'CwwG_G
Cuts at: 0 335 1012 1029
Size: 335 677 17
Fragments arranged by size:
677 335 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 225 1029
Size: 225 804
TatI w'GTAC_w
Cuts at: 0 611 809 1029
Size: 611 198 220
Fragments arranged by size:
611 220 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 649 681 1029
Size: 649 32 348
Fragments arranged by size:
649 348 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 208 709 1029
Size: 208 501 320
Fragments arranged by size:
501 320 208
XhoI C'TCGA_G
Cuts at: 0 571 1029
Size: 571 458
Enzymes that do cut:
Acc65I AcuI AflIII ApaI AvaI BamHI BanI BanII
BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BpmI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI
CspCI DrdI EaeI Eco57MI EcoNI EcoO109I Hin4I KpnI
MmeI MscI MslI MspA1I NcoI PasI PfoI PpuMI
PspOMI PspXI PstI PvuII SfcI SmlI StuI StyI
TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BciVI BfrBI BglII
BmrI BmtI BplI BpuEI BsaAI BsaBI BsiWI BsmI
BspEI BspHI BssHII BstBI BstEII BstZ17I BtgZI ClaI
DraI DraIII EagI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XmaI XmnI ZraI