Restriction map for
pFN21AE4332
(Linear) (Six-base) MAPSORT of: KIEE4332.seq Check: 6617 from: 1 to: 1023
KIEE4332.orf
With 182 enzymes: SgfI *
March 22, 2012 17:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 737 1023
Size: 737 286
AflIII A'CryG_T
Cuts at: 0 467 1023
Size: 467 556
AgeI A'CCGG_T
Cuts at: 0 712 1023
Size: 712 311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 296 1023
Size: 296 727
ApaI G_GGCC'C
Cuts at: 0 409 524 565 1023
Size: 409 115 41 458
Fragments arranged by size:
458 409 115 41
AvaI C'yCGr_G
Cuts at: 0 633 1023
Size: 633 390
BanII G_rGCy'C
Cuts at: 0 409 460 524 565 1022 1023
Size: 409 51 64 41 457 1
Fragments arranged by size:
457 409 64 51 41 1
BlpI GC'TnA_GC
Cuts at: 0 1016 1023
Size: 1016 7
Bme1580I G_kGCm'C
Cuts at: 0 409 524 565 1023
Size: 409 115 41 458
Fragments arranged by size:
458 409 115 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 335 1023
Size: 335 688
BsaI GGTCTCn'nnnn_
Cuts at: 0 243 1023
Size: 243 780
BsaWI w'CCGG_w
Cuts at: 0 712 1023
Size: 712 311
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 74 104 416 446 1023
Size: 74 30 312 30 577
Fragments arranged by size:
577 312 74 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 766 769 1023
Size: 766 3 254
Fragments arranged by size:
766 254 3
BseYI C'CCAG_C
Cuts at: 0 398 1023
Size: 398 625
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1023
Size: 163 860
BsiHKAI G_wGCw'C
Cuts at: 0 227 460 1023
Size: 227 233 563
Fragments arranged by size:
563 233 227
Bsp1286I G_dGCh'C
Cuts at: 0 227 409 460 524 565 1022 1023
Size: 227 182 51 64 41 457 1
Fragments arranged by size:
457 227 182 64 51 41 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 737 1023
Size: 737 286
BsrDI GCAATG_nn'
Cuts at: 0 148 1023
Size: 148 875
BsrFI r'CCGG_y
Cuts at: 0 712 1023
Size: 712 311
BsrGI T'GTAC_A
Cuts at: 0 995 1023
Size: 995 28
BstEII G'GTnAC_C
Cuts at: 0 715 1023
Size: 715 308
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 623 1023
Size: 417 206 400
Fragments arranged by size:
417 400 206
Bsu36I CC'TnA_GG
Cuts at: 0 893 1023
Size: 893 130
BtgI C'CryG_G
Cuts at: 0 410 573 1023
Size: 410 163 450
Fragments arranged by size:
450 410 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 781 1023
Size: 781 242
BtsI GCAGTG_nn'
Cuts at: 0 643 921 1023
Size: 643 278 102
Fragments arranged by size:
643 278 102
EaeI y'GGCC_r
Cuts at: 0 58 1023
Size: 58 965
EarI CTCTTCn'nnn_
Cuts at: 0 119 772 787 1023
Size: 119 653 15 236
Fragments arranged by size:
653 236 119 15
EcoICRI GAG'CTC
Cuts at: 0 458 1023
Size: 458 565
EcoO109I rG'GnC_Cy
Cuts at: 0 53 106 287 406 520 562 746 1023
Size: 53 53 181 119 114 42 184 277
Fragments arranged by size:
277 184 181 119 114 53 53 42
EcoRV GAT'ATC
Cuts at: 0 987 1023
Size: 987 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 267 1023
Size: 267 756
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 1023
Size: 223 32 768
Fragments arranged by size:
768 223 32
HincII GTy'rAC
Cuts at: 0 531 1023
Size: 531 492
MscI TGG'CCA
Cuts at: 0 60 1023
Size: 60 963
MslI CAynn'nnrTG
Cuts at: 0 230 621 731 1023
Size: 230 391 110 292
Fragments arranged by size:
391 292 230 110
NdeI CA'TA_TG
Cuts at: 0 906 1023
Size: 906 117
NspI r_CATG'y
Cuts at: 0 471 736 1023
Size: 471 265 287
Fragments arranged by size:
471 287 265
PciI A'CATG_T
Cuts at: 0 467 1023
Size: 467 556
PfoI T'CCnGG_A
Cuts at: 0 451 1023
Size: 451 572
PpuMI rG'GwC_Cy
Cuts at: 0 106 287 746 1023
Size: 106 181 459 277
Fragments arranged by size:
459 277 181 106
PspOMI G'GGCC_C
Cuts at: 0 405 520 561 1023
Size: 405 115 41 462
Fragments arranged by size:
462 405 115 41
PspXI vC'TCGA_Gb
Cuts at: 0 633 1023
Size: 633 390
PstI C_TGCA'G
Cuts at: 0 50 448 519 849 1023
Size: 50 398 71 330 174
Fragments arranged by size:
398 330 174 71 50
SacI G_AGCT'C
Cuts at: 0 460 1023
Size: 460 563
SbfI CC_TGCA'GG
Cuts at: 0 849 1023
Size: 849 174
SfcI C'TryA_G
Cuts at: 0 46 291 444 515 585 845 1023
Size: 46 245 153 71 70 260 178
Fragments arranged by size:
260 245 178 153 71 70 46
SmlI C'TyrA_G
Cuts at: 0 633 1023
Size: 633 390
StyI C'CwwG_G
Cuts at: 0 242 524 1023
Size: 242 282 499
Fragments arranged by size:
499 282 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 116 250 586 705 1023
Size: 116 134 336 119 318
Fragments arranged by size:
336 318 134 119 116
TatI w'GTAC_w
Cuts at: 0 995 1023
Size: 995 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 990 1022 1023
Size: 416 32 542 32 1
Fragments arranged by size:
542 416 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 0 358 438 1023
Size: 358 80 585
Fragments arranged by size:
585 358 80
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 348 1023
Size: 348 675
XhoI C'TCGA_G
Cuts at: 0 633 1023
Size: 633 390
XmnI GAAnn'nnTTC
Cuts at: 0 84 669 1023
Size: 84 585 354
Fragments arranged by size:
585 354 84
Enzymes that do cut:
AarI AflIII AgeI AlfI ApaI AvaI BanII BlpI
Bme1580I BmrI BsaI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI BstEII BstXI
Bsu36I BtgI BtgZI BtsI EaeI EarI EcoICRI EcoO109I
EcoRV FalI Hin4I HincII MscI MslI NdeI NspI
PciI PfoI PpuMI PspOMI PspXI PstI SacI SbfI
SfcI SmlI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AhdI
AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BssHII BssSI BstAPI
BstBI BstYI BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EciI Eco57MI EcoNI EcoRI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI
PsiI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI
XmaI ZraI