Restriction map for
pFN21AE4330
(Linear) (Six-base) MAPSORT of: KIEE4330.seq Check: 1636 from: 1 to: 1014
KIEE4330.orf
With 182 enzymes: SgfI *
March 22, 2012 17:45 ..
AatII G_ACGT'C
Cuts at: 0 508 1014
Size: 508 506
AccI GT'mk_AC
Cuts at: 0 136 1014
Size: 136 878
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 375 531 670 1014
Size: 74 301 156 139 344
Fragments arranged by size:
344 301 156 139 74
AhdI GACnn_n'nnGTC
Cuts at: 0 509 1014
Size: 509 505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 852 1014
Size: 852 162
AlwNI CAG_nnn'CTG
Cuts at: 0 398 493 531 1014
Size: 398 95 38 483
Fragments arranged by size:
483 398 95 38
ApaLI G'TGCA_C
Cuts at: 0 736 1014
Size: 736 278
ApoI r'AATT_y
Cuts at: 0 175 928 1014
Size: 175 753 86
Fragments arranged by size:
753 175 86
AvaI C'yCGr_G
Cuts at: 0 409 731 1014
Size: 409 322 283
Fragments arranged by size:
409 322 283
BamHI G'GATC_C
Cuts at: 0 704 1014
Size: 704 310
BanI G'GyrC_C
Cuts at: 0 880 1014
Size: 880 134
BanII G_rGCy'C
Cuts at: 0 389 646 984 1014
Size: 389 257 338 30
Fragments arranged by size:
389 338 257 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 508 1014
Size: 508 506
BclI T'GATC_A
Cuts at: 0 350 1014
Size: 350 664
BglI GCCn_nnn'nGGC
Cuts at: 0 50 580 1014
Size: 50 530 434
Fragments arranged by size:
530 434 50
Bme1580I G_kGCm'C
Cuts at: 0 740 883 1014
Size: 740 143 131
Fragments arranged by size:
740 143 131
BmgBI CAC'GTC
Cuts at: 0 873 1014
Size: 873 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 1014
Size: 470 544
BsaHI Gr'CG_yC
Cuts at: 0 505 1014
Size: 505 509
BsaWI w'CCGG_w
Cuts at: 0 821 1014
Size: 821 193
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 628 658 1014
Size: 628 30 356
Fragments arranged by size:
628 356 30
BseYI C'CCAG_C
Cuts at: 0 553 1014
Size: 553 461
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 505 517 1014
Size: 384 121 12 497
Fragments arranged by size:
497 384 121 12
BsiEI CG_ry'CG
Cuts at: 0 54 65 1014
Size: 54 11 949
Fragments arranged by size:
949 54 11
BsiHKAI G_wGCw'C
Cuts at: 0 389 593 626 740 984 1014
Size: 389 204 33 114 244 30
Fragments arranged by size:
389 244 204 114 33 30
Bsp1286I G_dGCh'C
Cuts at: 0 389 593 626 646 740 883 984 1014
Size: 389 204 33 20 94 143 101 30
Fragments arranged by size:
389 204 143 101 94 33 30 20
BspEI T'CCGG_A
Cuts at: 0 821 1014
Size: 821 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 539 1014
Size: 539 475
BstBI TT'CG_AA
Cuts at: 0 319 1014
Size: 319 695
BstXI CCAn_nnnn'nTGG
Cuts at: 0 796 1014
Size: 796 218
BstYI r'GATC_y
Cuts at: 0 704 793 1014
Size: 704 89 221
Fragments arranged by size:
704 221 89
BstZ17I GTA'TAC
Cuts at: 0 137 1014
Size: 137 877
BtgI C'CryG_G
Cuts at: 0 699 789 1014
Size: 699 90 225
Fragments arranged by size:
699 225 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 598 1014
Size: 598 416
DraIII CAC_nnn'GTG
Cuts at: 0 744 1014
Size: 744 270
EaeI y'GGCC_r
Cuts at: 0 51 62 455 1014
Size: 51 11 393 559
Fragments arranged by size:
559 393 51 11
EagI C'GGCC_G
Cuts at: 0 51 62 1014
Size: 51 11 952
Fragments arranged by size:
952 51 11
EarI CTCTTCn'nnn_
Cuts at: 0 394 1014
Size: 394 620
EcoICRI GAG'CTC
Cuts at: 0 387 982 1014
Size: 387 595 32
Fragments arranged by size:
595 387 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 375 470 531 670 1014
Size: 74 301 95 61 139 344
Fragments arranged by size:
344 301 139 95 74 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 523 1014
Size: 523 491
EcoO109I rG'GnC_Cy
Cuts at: 0 528 1014
Size: 528 486
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 405 1014
Size: 405 609
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 495 527 1014
Size: 495 32 487
Fragments arranged by size:
495 487 32
HincII GTy'rAC
Cuts at: 0 687 1014
Size: 687 327
HpaI GTT'AAC
Cuts at: 0 687 1014
Size: 687 327
MscI TGG'CCA
Cuts at: 0 457 1014
Size: 457 557
MslI CAynn'nnrTG
Cuts at: 0 871 1014
Size: 871 143
PasI CC'CwG_GG
Cuts at: 0 813 1014
Size: 813 201
PflMI CCAn_nnn'nTGG
Cuts at: 0 493 1014
Size: 493 521
PfoI T'CCnGG_A
Cuts at: 0 362 371 707 1014
Size: 362 9 336 307
Fragments arranged by size:
362 336 307 9
PpuMI rG'GwC_Cy
Cuts at: 0 528 1014
Size: 528 486
PstI C_TGCA'G
Cuts at: 0 32 268 1014
Size: 32 236 746
Fragments arranged by size:
746 236 32
SacI G_AGCT'C
Cuts at: 0 389 984 1014
Size: 389 595 30
Fragments arranged by size:
595 389 30
SapI GCTCTTCn'nnn_
Cuts at: 0 394 1014
Size: 394 620
SexAI A'CCwGG_T
Cuts at: 0 740 1014
Size: 740 274
SfcI C'TryA_G
Cuts at: 0 28 264 1014
Size: 28 236 750
Fragments arranged by size:
750 236 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 50 1014
Size: 50 964
SmaI CCC'GGG
Cuts at: 0 733 1014
Size: 733 281
SspI AAT'ATT
Cuts at: 0 450 1014
Size: 450 564
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 628 660 1014
Size: 628 32 354
Fragments arranged by size:
628 354 32
XmaI C'CCGG_G
Cuts at: 0 731 1014
Size: 731 283
ZraI GAC'GTC
Cuts at: 0 506 1014
Size: 506 508
Enzymes that do cut:
AatII AccI AcuI AhdI AlfI AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BbsI BclI BglI Bme1580I
BmgBI BpmI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BstBI BstXI BstYI BstZ17I
BtgI BtgZI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI Hin4I HincII HpaI MscI MslI
PasI PflMI PfoI PpuMI PstI SacI SapI SexAI
SfcI SfiI SmaI SspI TstI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AleI
AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BfrBI BglII BlpI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI BsiWI
BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstEII Bsu36I BtsI ClaI CspCI DraI
DrdI EciI EcoRI EcoRV FseI FspI FspAI HaeII
HindIII KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI StuI StyI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmnI