(Linear) (Six-base) MAPSORT of: KIEE4329.seq  Check: 578  from: 1  to: 1002



KIEE4329.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:45  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     613    1002 
   Size:        613     389
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202    1002 
   Size:        202     800
 
AflII C'TTAA_G

Cuts at:      0      10    1002 
   Size:         10     992
 
AleI CACnn'nnGTG

Cuts at:      0      76    1002 
   Size:         76     926
 
AlwNI CAG_nnn'CTG

Cuts at:      0     554    1002 
   Size:        554     448
 
ApoI r'AATT_y

Cuts at:      0     355    1002 
   Size:        355     647
 
AvaI C'yCGr_G

Cuts at:      0     893    1002 
   Size:        893     109
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     342     855    1002 
   Size:        342     513     147

  Fragments arranged by size: 

                 513     342     147
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     892    1002 
   Size:        892     110
 
BlpI GC'TnA_GC

Cuts at:      0     944    1002 
   Size:        944      58
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     412     759    1002 
   Size:        412     347     243

  Fragments arranged by size: 

                 412     347     243
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619    1002 
   Size:        619     383
 
Bpu10I CC'TnA_GC

Cuts at:      0     868    1002 
   Size:        868     134
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     573     934    1002 
   Size:        573     361      68

  Fragments arranged by size: 

                 573     361      68
 
BsaAI yAC'GTr

Cuts at:      0     434    1002 
   Size:        434     568
 
BseYI C'CCAG_C

Cuts at:      0     547    1002 
   Size:        547     455
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     980    1002 
   Size:        352     628      22

  Fragments arranged by size: 

                 628     352      22
 
BsmI GAATG_Cn'

Cuts at:      0     116     539    1002 
   Size:        116     423     463

  Fragments arranged by size: 

                 463     423     116
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     613    1002 
   Size:        613     389
 
BsrDI GCAATG_nn'

Cuts at:      0      23    1002 
   Size:         23     979
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      34    1002 
   Size:         34     968
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     420    1002 
   Size:        420     582
 
BstYI r'GATC_y

Cuts at:      0     192    1002 
   Size:        192     810
 
BtgI C'CryG_G

Cuts at:      0     558    1002 
   Size:        558     444
 
DraIII CAC_nnn'GTG

Cuts at:      0     474    1002 
   Size:        474     528
 
EarI CTCTTCn'nnn_

Cuts at:      0      38    1002 
   Size:         38     964
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     619    1002 
   Size:        202     417     383

  Fragments arranged by size: 

                 417     383     202
 
EcoO109I rG'GnC_Cy

Cuts at:      0     987    1002 
   Size:        987      15
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     190    1002 
   Size:        190     812
 
HincII GTy'rAC

Cuts at:      0     824    1002 
   Size:        824     178
 
HindIII A'AGCT_T

Cuts at:      0     172    1002 
   Size:        172     830
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     437    1002 
   Size:         66     371     565

  Fragments arranged by size: 

                 565     371      66
 
MslI CAynn'nnrTG

Cuts at:      0      76     403    1002 
   Size:         76     327     599

  Fragments arranged by size: 

                 599     327      76
 
MspA1I CmG'CkG

Cuts at:      0     554    1002 
   Size:        554     448
 
NcoI C'CATG_G

Cuts at:      0     558    1002 
   Size:        558     444
 
PfoI T'CCnGG_A

Cuts at:      0     635     805    1002 
   Size:        635     170     197

  Fragments arranged by size: 

                 635     197     170
 
PpuMI rG'GwC_Cy

Cuts at:      0     987    1002 
   Size:        987      15
 
PstI C_TGCA'G

Cuts at:      0     200     291    1002 
   Size:        200      91     711

  Fragments arranged by size: 

                 711     200      91
 
PvuII CAG'CTG

Cuts at:      0     554    1002 
   Size:        554     448
 
SfcI C'TryA_G

Cuts at:      0      74     196     287    1002 
   Size:         74     122      91     715

  Fragments arranged by size: 

                 715     122      91      74
 
SmaI CCC'GGG

Cuts at:      0     895    1002 
   Size:        895     107
 
SmlI C'TyrA_G

Cuts at:      0      10     588     949    1002 
   Size:         10     578     361      53

  Fragments arranged by size: 

                 578     361      53      10
 
SnaBI TAC'GTA

Cuts at:      0     434    1002 
   Size:        434     568
 
StyI C'CwwG_G

Cuts at:      0     558    1002 
   Size:        558     444
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      70    1002 
   Size:         70     932
 
TatI w'GTAC_w

Cuts at:      0     380    1002 
   Size:        380     622
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     438     470     752     784    1002 
   Size:        438      32     282      32     218

  Fragments arranged by size: 

                 438     282     218      32      32
 
XmaI C'CCGG_G

Cuts at:      0     893    1002 
   Size:        893     109
 

 Enzymes that do cut:

     AarI     AcuI    AflII     AleI    AlwNI     ApoI     AvaI     BbsI
    BciVI     BlpI     BmrI     BpmI   Bpu10I    BpuEI    BsaAI    BseYI
     BsgI     BsmI    BspMI    BsrDI   BstAPI    BstXI    BstYI     BtgI
   DraIII     EarI  Eco57MI EcoO109I     FalI   HincII  HindIII     MmeI
     MslI   MspA1I     NcoI     PfoI    PpuMI     PstI    PvuII     SfcI
     SmaI     SmlI    SnaBI     StyI    TaqII     TatI     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI     BclI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI     BplI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI