Restriction map for
pFN21AE4326
(Linear) (Six-base) MAPSORT of: KIEE4326.seq Check: 6326 from: 1 to: 984
KIEE4326.orf
With 182 enzymes: SgfI *
March 22, 2012 17:44 ..
AccI GT'mk_AC
Cuts at: 0 308 984
Size: 308 676
AflIII A'CryG_T
Cuts at: 0 512 984
Size: 512 472
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 109 141 984
Size: 109 32 843
Fragments arranged by size:
843 109 32
AlwNI CAG_nnn'CTG
Cuts at: 0 333 378 699 711 984
Size: 333 45 321 12 273
Fragments arranged by size:
333 321 273 45 12
ApaI G_GGCC'C
Cuts at: 0 173 754 984
Size: 173 581 230
Fragments arranged by size:
581 230 173
ApaLI G'TGCA_C
Cuts at: 0 964 984
Size: 964 20
AvaI C'yCGr_G
Cuts at: 0 148 351 919 984
Size: 148 203 568 65
Fragments arranged by size:
568 203 148 65
BanI G'GyrC_C
Cuts at: 0 217 427 984
Size: 217 210 557
Fragments arranged by size:
557 217 210
BanII G_rGCy'C
Cuts at: 0 173 242 358 653 754 900 984
Size: 173 69 116 295 101 146 84
Fragments arranged by size:
295 173 146 116 101 84 69
BbeI G_GCGC'C
Cuts at: 0 221 431 984
Size: 221 210 553
Fragments arranged by size:
553 221 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 937 971 984
Size: 937 34 13
Fragments arranged by size:
937 34 13
BglI GCCn_nnn'nGGC
Cuts at: 0 199 208 984
Size: 199 9 776
Fragments arranged by size:
776 199 9
Bme1580I G_kGCm'C
Cuts at: 0 173 397 754 968 984
Size: 173 224 357 214 16
Fragments arranged by size:
357 224 214 173 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 91 984
Size: 91 893
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 738 984
Size: 738 246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 664 733 778 984
Size: 307 357 69 45 206
Fragments arranged by size:
357 307 206 69 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 984
Size: 535 449
BsaAI yAC'GTr
Cuts at: 0 495 984
Size: 495 489
BsaHI Gr'CG_yC
Cuts at: 0 218 305 416 428 984
Size: 218 87 111 12 556
Fragments arranged by size:
556 218 111 87 12
BsaWI w'CCGG_w
Cuts at: 0 738 984
Size: 738 246
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 340 370 467 497 754 784
Size: 109 30 201 30 97 30 257 30
Cuts at: 784 844 874 984
Size: 60 30 110
Fragments arranged by size:
257 201 110 109 97 60 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 661 984
Size: 661 323
BseYI C'CCAG_C
Cuts at: 0 971 984
Size: 971 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 760 984
Size: 126 634 224
Fragments arranged by size:
634 224 126
BsiHKAI G_wGCw'C
Cuts at: 0 242 764 968 984
Size: 242 522 204 16
Fragments arranged by size:
522 242 204 16
Bsp1286I G_dGCh'C
Cuts at: 0 173 242 358 397 653 754 764 900
Size: 173 69 116 39 256 101 10 136
Cuts at: 900 968 984
Size: 68 16
Fragments arranged by size:
256 173 136 116 101 69 68 39
16 10
BspEI T'CCGG_A
Cuts at: 0 738 984
Size: 738 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 432 984
Size: 432 552
BsrBI CCG'CTC
Cuts at: 0 717 747 984
Size: 717 30 237
Fragments arranged by size:
717 237 30
BsrFI r'CCGG_y
Cuts at: 0 177 605 984
Size: 177 428 379
Fragments arranged by size:
428 379 177
BsrGI T'GTAC_A
Cuts at: 0 806 984
Size: 806 178
BssHII G'CGCG_C
Cuts at: 0 109 111 984
Size: 109 2 873
Fragments arranged by size:
873 109 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 230 984
Size: 230 754
BstYI r'GATC_y
Cuts at: 0 719 984
Size: 719 265
BtgI C'CryG_G
Cuts at: 0 194 984
Size: 194 790
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 473 984
Size: 473 511
BtsI GCAGTG_nn'
Cuts at: 0 212 537 984
Size: 212 325 447
Fragments arranged by size:
447 325 212
EaeI y'GGCC_r
Cuts at: 0 158 200 209 324 537 572 984
Size: 158 42 9 115 213 35 412
Fragments arranged by size:
412 213 158 115 42 35 9
EarI CTCTTCn'nnn_
Cuts at: 0 937 984
Size: 937 47
EcoICRI GAG'CTC
Cuts at: 0 240 984
Size: 240 744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 664 733 778 984
Size: 307 357 69 45 206
Fragments arranged by size:
357 307 206 69 45
EcoO109I rG'GnC_Cy
Cuts at: 0 170 708 750 751 915 984
Size: 170 538 42 1 164 69
Fragments arranged by size:
538 170 164 69 42 1
HaeII r_GCGC'y
Cuts at: 0 156 221 379 411 431 984
Size: 156 65 158 32 20 553
Fragments arranged by size:
553 158 156 65 32 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 984
Size: 451 32 501
Fragments arranged by size:
501 451 32
KasI G'GCGC_C
Cuts at: 0 217 427 984
Size: 217 210 557
Fragments arranged by size:
557 217 210
MslI CAynn'nnrTG
Cuts at: 0 511 984
Size: 511 473
MspA1I CmG'CkG
Cuts at: 0 33 48 57 66 131 196 975 984
Size: 33 15 9 9 65 65 779 9
Fragments arranged by size:
779 65 65 33 15 9 9 9
NarI GG'CG_CC
Cuts at: 0 218 428 984
Size: 218 210 556
Fragments arranged by size:
556 218 210
NspI r_CATG'y
Cuts at: 0 228 516 984
Size: 228 288 468
Fragments arranged by size:
468 288 228
PciI A'CATG_T
Cuts at: 0 512 984
Size: 512 472
PflMI CCAn_nnn'nTGG
Cuts at: 0 711 984
Size: 711 273
PfoI T'CCnGG_A
Cuts at: 0 245 581 710 984
Size: 245 336 129 274
Fragments arranged by size:
336 274 245 129
PmlI CAC'GTG
Cuts at: 0 495 984
Size: 495 489
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 984
Size: 109 32 843
Fragments arranged by size:
843 109 32
PpuMI rG'GwC_Cy
Cuts at: 0 708 915 984
Size: 708 207 69
Fragments arranged by size:
708 207 69
PspOMI G'GGCC_C
Cuts at: 0 169 750 984
Size: 169 581 234
Fragments arranged by size:
581 234 169
PstI C_TGCA'G
Cuts at: 0 374 984
Size: 374 610
SacI G_AGCT'C
Cuts at: 0 242 984
Size: 242 742
SacII CC_GC'GG
Cuts at: 0 197 984
Size: 197 787
SanDI GG'GwC_CC
Cuts at: 0 915 984
Size: 915 69
SbfI CC_TGCA'GG
Cuts at: 0 374 984
Size: 374 610
SexAI A'CCwGG_T
Cuts at: 0 704 984
Size: 704 280
SfcI C'TryA_G
Cuts at: 0 370 984
Size: 370 614
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 208 984
Size: 208 776
SfoI GGC'GCC
Cuts at: 0 219 429 984
Size: 219 210 555
Fragments arranged by size:
555 219 210
SmaI CCC'GGG
Cuts at: 0 353 984
Size: 353 631
SmlI C'TyrA_G
Cuts at: 0 550 984
Size: 550 434
SphI G_CATG'C
Cuts at: 0 228 984
Size: 228 756
StuI AGG'CCT
Cuts at: 0 479 984
Size: 479 505
StyI C'CwwG_G
Cuts at: 0 636 984
Size: 636 348
TatI w'GTAC_w
Cuts at: 0 806 984
Size: 806 178
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 340 372 467 499 754 786 844 876
Size: 340 32 95 32 255 32 58 32
Cuts at: 876 907 939 984
Size: 31 32 45
Fragments arranged by size:
340 255 95 58 45 32 32 32
32 32 31
Tth111I GACn'n_nGTC
Cuts at: 0 706 984
Size: 706 278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 513 984
Size: 513 471
XmaI C'CCGG_G
Cuts at: 0 351 984
Size: 351 633
Enzymes that do cut:
AccI AflIII AloI AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BcgI BglI Bme1580I BmrI BplI BpmI
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstYI BtgI BtgZI BtsI EaeI EarI EcoICRI
Eco57MI EcoO109I HaeII Hin4I KasI MslI MspA1I NarI
NspI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI
PstI SacI SacII SanDI SbfI SexAI SfcI SfiI
SfoI SmaI SmlI SphI StuI StyI TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI Bpu10I BsaI BsaBI BsiEI BsiWI
BsmI BsmBI BspHI BsrDI BssSI BstBI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SalI SapI ScaI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI TaqII XbaI XhoI XmnI
ZraI