Restriction map for
pFN21AE4325
(Linear) (Six-base) MAPSORT of: KIEE4325.seq Check: 9023 from: 1 to: 984
KIEE4325.orf
With 182 enzymes: SgfI *
March 22, 2012 17:44 ..
AclI AA'CG_TT
Cuts at: 0 940 984
Size: 940 44
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 892 984
Size: 391 501 92
Fragments arranged by size:
501 391 92
AhdI GACnn_n'nnGTC
Cuts at: 0 967 984
Size: 967 17
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 156 984
Size: 156 828
AlwNI CAG_nnn'CTG
Cuts at: 0 240 369 984
Size: 240 129 615
Fragments arranged by size:
615 240 129
ApoI r'AATT_y
Cuts at: 0 678 984
Size: 678 306
AseI AT'TA_AT
Cuts at: 0 136 984
Size: 136 848
AvaI C'yCGr_G
Cuts at: 0 68 105 984
Size: 68 37 879
Fragments arranged by size:
879 68 37
BanI G'GyrC_C
Cuts at: 0 205 984
Size: 205 779
BanII G_rGCy'C
Cuts at: 0 857 984
Size: 857 127
BglII A'GATC_T
Cuts at: 0 413 984
Size: 413 571
Bme1580I G_kGCm'C
Cuts at: 0 208 519 984
Size: 208 311 465
Fragments arranged by size:
465 311 208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 984
Size: 505 479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 984
Size: 572 412
BsaBI GATnn'nnATC
Cuts at: 0 412 984
Size: 412 572
BsaWI w'CCGG_w
Cuts at: 0 88 984
Size: 88 896
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 709 739 984
Size: 709 30 245
Fragments arranged by size:
709 245 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 68 984
Size: 68 916
BseYI C'CCAG_C
Cuts at: 0 865 984
Size: 865 119
Bsp1286I G_dGCh'C
Cuts at: 0 208 519 857 984
Size: 208 311 338 127
Fragments arranged by size:
338 311 208 127
BstEII G'GTnAC_C
Cuts at: 0 696 984
Size: 696 288
BstXI CCAn_nnnn'nTGG
Cuts at: 0 644 984
Size: 644 340
BstYI r'GATC_y
Cuts at: 0 323 407 413 509 984
Size: 323 84 6 96 475
Fragments arranged by size:
475 323 96 84 6
DraI TTT'AAA
Cuts at: 0 500 984
Size: 500 484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 505 892 984
Size: 391 114 387 92
Fragments arranged by size:
391 387 114 92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 175 984
Size: 175 809
EcoO109I rG'GnC_Cy
Cuts at: 0 18 984
Size: 18 966
EcoRI G'AATT_C
Cuts at: 0 678 984
Size: 678 306
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 630 984
Size: 630 354
FspI TGC'GCA
Cuts at: 0 29 984
Size: 29 955
FspAI rTGC'GCAy
Cuts at: 0 29 984
Size: 29 955
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 953 984
Size: 953 31
MspA1I CmG'CkG
Cuts at: 0 313 736 984
Size: 313 423 248
Fragments arranged by size:
423 313 248
PfoI T'CCnGG_A
Cuts at: 0 673 984
Size: 673 311
PshAI GACnn'nnGTC
Cuts at: 0 151 229 984
Size: 151 78 755
Fragments arranged by size:
755 151 78
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 604 636 984
Size: 604 32 348
Fragments arranged by size:
604 348 32
PstI C_TGCA'G
Cuts at: 0 179 984
Size: 179 805
PvuII CAG'CTG
Cuts at: 0 313 984
Size: 313 671
ScaI AGT'ACT
Cuts at: 0 601 984
Size: 601 383
SexAI A'CCwGG_T
Cuts at: 0 643 984
Size: 643 341
SfcI C'TryA_G
Cuts at: 0 175 574 984
Size: 175 399 410
Fragments arranged by size:
410 399 175
SmaI CCC'GGG
Cuts at: 0 107 984
Size: 107 877
SmlI C'TyrA_G
Cuts at: 0 587 984
Size: 587 397
StuI AGG'CCT
Cuts at: 0 913 984
Size: 913 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 132 786 984
Size: 132 654 198
Fragments arranged by size:
654 198 132
TatI w'GTAC_w
Cuts at: 0 599 984
Size: 599 385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 107 139 834 866 984
Size: 107 32 695 32 118
Fragments arranged by size:
695 118 107 32 32
XmaI C'CCGG_G
Cuts at: 0 105 984
Size: 105 879
XmnI GAAnn'nnTTC
Cuts at: 0 601 984
Size: 601 383
Enzymes that do cut:
AclI AcuI AhdI AlfI AlwNI ApoI AseI AvaI
BanI BanII BglII Bme1580I BpmI BpuEI BsaBI BsaWI
BsaXI BseRI BseYI Bsp1286I BstEII BstXI BstYI DraI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI Hin4I
MspA1I PfoI PshAI PsrI PstI PvuII ScaI SexAI
SfcI SmaI SmlI StuI TaqII TatI TstI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AleI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EagI EarI EciI
EcoICRI EcoRV FseI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StyI SwaI Tth111I XbaI
XcmI XhoI ZraI