Restriction map for
pFN21AE4323
(Linear) (Six-base) MAPSORT of: KIEE4323.seq Check: 1595 from: 1 to: 978
KIEE4323.orf
With 182 enzymes: SgfI *
July 3, 2012 18:57 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 782 828 978
Size: 782 46 150
Fragments arranged by size:
782 150 46
ApaI G_GGCC'C
Cuts at: 0 253 791 978
Size: 253 538 187
Fragments arranged by size:
538 253 187
AvaI C'yCGr_G
Cuts at: 0 102 522 978
Size: 102 420 456
Fragments arranged by size:
456 420 102
BamHI G'GATC_C
Cuts at: 0 832 978
Size: 832 146
BanI G'GyrC_C
Cuts at: 0 207 709 978
Size: 207 502 269
Fragments arranged by size:
502 269 207
BanII G_rGCy'C
Cuts at: 0 43 100 124 253 791 880 978
Size: 43 57 24 129 538 89 98
Fragments arranged by size:
538 129 98 89 57 43 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 515 885 978
Size: 515 370 93
Fragments arranged by size:
515 370 93
BglI GCCn_nnn'nGGC
Cuts at: 0 164 473 611 875 978
Size: 164 309 138 264 103
Fragments arranged by size:
309 264 164 138 103
BlpI GC'TnA_GC
Cuts at: 0 37 978
Size: 37 941
Bme1580I G_kGCm'C
Cuts at: 0 53 212 253 791 978
Size: 53 159 41 538 187
Fragments arranged by size:
538 187 159 53 41
BmgBI CAC'GTC
Cuts at: 0 48 978
Size: 48 930
BmrI ACTGGGnnnn_n'
Cuts at: 0 733 978
Size: 733 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 132 439 612 630 978
Size: 117 15 307 173 18 348
Fragments arranged by size:
348 307 173 117 18 15
Bpu10I CC'TnA_GC
Cuts at: 0 118 254 978
Size: 118 136 724
Fragments arranged by size:
724 136 118
BsaI GGTCTCn'nnnn_
Cuts at: 0 722 978
Size: 722 256
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 90 978
Size: 90 888
BseYI C'CCAG_C
Cuts at: 0 92 203 920 978
Size: 92 111 717 58
Fragments arranged by size:
717 111 92 58
Bsp1286I G_dGCh'C
Cuts at: 0 43 53 100 124 212 253 791 880
Size: 43 10 47 24 88 41 538 89
Cuts at: 880 978
Size: 98
Fragments arranged by size:
538 98 89 88 47 43 41 24
10
BsrBI CCG'CTC
Cuts at: 0 170 978
Size: 170 808
BsrFI r'CCGG_y
Cuts at: 0 610 978
Size: 610 368
BsrGI T'GTAC_A
Cuts at: 0 819 978
Size: 819 159
BstEII G'GTnAC_C
Cuts at: 0 147 978
Size: 147 831
BstXI CCAn_nnnn'nTGG
Cuts at: 0 183 978
Size: 183 795
BstYI r'GATC_y
Cuts at: 0 3 832 978
Size: 3 829 146
Fragments arranged by size:
829 146 3
BtgI C'CryG_G
Cuts at: 0 66 381 926 978
Size: 66 315 545 52
Fragments arranged by size:
545 315 66 52
BtsI GCAGTG_nn'
Cuts at: 0 281 978
Size: 281 697
DrdI GACnn_nn'nnGTC
Cuts at: 0 889 978
Size: 889 89
EaeI y'GGCC_r
Cuts at: 0 165 576 608 978
Size: 165 411 32 370
Fragments arranged by size:
411 370 165 32
EarI CTCTTCn'nnn_
Cuts at: 0 720 978
Size: 720 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 132 439 612 630 978
Size: 117 15 307 173 18 348
Fragments arranged by size:
348 307 173 117 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 184 759 978
Size: 184 575 219
Fragments arranged by size:
575 219 184
EcoO109I rG'GnC_Cy
Cuts at: 0 106 250 669 787 788 978
Size: 106 144 419 118 1 190
Fragments arranged by size:
419 190 144 118 106 1
FseI GG_CCGG'CC
Cuts at: 0 614 978
Size: 614 364
HaeII r_GCGC'y
Cuts at: 0 351 978
Size: 351 627
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 935 967 978
Size: 935 32 11
Fragments arranged by size:
935 32 11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 152 307 860 978
Size: 86 66 155 553 118
Fragments arranged by size:
553 155 118 86 66
MspA1I CmG'CkG
Cuts at: 0 197 234 464 860 978
Size: 197 37 230 396 118
Fragments arranged by size:
396 230 197 118 37
NaeI GCC'GGC
Cuts at: 0 612 978
Size: 612 366
NcoI C'CATG_G
Cuts at: 0 66 381 978
Size: 66 315 597
Fragments arranged by size:
597 315 66
NgoMIV G'CCGG_C
Cuts at: 0 610 978
Size: 610 368
PasI CC'CwG_GG
Cuts at: 0 828 978
Size: 828 150
PflMI CCAn_nnn'nTGG
Cuts at: 0 220 387 664 927 978
Size: 220 167 277 263 51
Fragments arranged by size:
277 263 220 167 51
PpuMI rG'GwC_Cy
Cuts at: 0 106 669 978
Size: 106 563 309
Fragments arranged by size:
563 309 106
PspOMI G'GGCC_C
Cuts at: 0 249 787 978
Size: 249 538 191
Fragments arranged by size:
538 249 191
PvuII CAG'CTG
Cuts at: 0 197 978
Size: 197 781
SanDI GG'GwC_CC
Cuts at: 0 106 978
Size: 106 872
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 611 978
Size: 611 367
SmaI CCC'GGG
Cuts at: 0 524 978
Size: 524 454
StuI AGG'CCT
Cuts at: 0 605 978
Size: 605 373
StyI C'CwwG_G
Cuts at: 0 66 381 791 978
Size: 66 315 410 187
Fragments arranged by size:
410 315 187 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 195 285 784 978
Size: 195 90 499 194
Fragments arranged by size:
499 195 194 90
TatI w'GTAC_w
Cuts at: 0 819 978
Size: 819 159
Tth111I GACn'n_nGTC
Cuts at: 0 78 978
Size: 78 900
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 379 978
Size: 180 199 599
Fragments arranged by size:
599 199 180
XmaI C'CCGG_G
Cuts at: 0 522 978
Size: 522 456
Enzymes that do cut:
AlwNI ApaI AvaI BamHI BanI BanII BbsI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BseRI
BseYI Bsp1286I BsrBI BsrFI BsrGI BstEII BstXI BstYI
BtgI BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I
FseI HaeII Hin4I MmeI MspA1I NaeI NcoI NgoMIV
PasI PflMI PpuMI PspOMI PvuII SanDI SfiI SmaI
StuI StyI TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BmtI BplI BpuEI BsaAI
BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EagI EciI EcoICRI EcoRI EcoRV FalI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MslI NarI NdeI NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TstI XbaI XhoI XmnI ZraI