Restriction map for
pFN21AE4321
(Linear) (Six-base) MAPSORT of: KIEE4321.seq Check: 822 from: 1 to: 975
KIEE4321.orf
With 182 enzymes: SgfI *
March 22, 2012 17:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 345 975
Size: 345 630
AccI GT'mk_AC
Cuts at: 0 908 975
Size: 908 67
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 975
Size: 961 14
AfeI AGC'GCT
Cuts at: 0 188 975
Size: 188 787
AleI CACnn'nnGTG
Cuts at: 0 670 975
Size: 670 305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 835 975
Size: 835 140
ApaI G_GGCC'C
Cuts at: 0 43 975
Size: 43 932
ApaLI G'TGCA_C
Cuts at: 0 810 975
Size: 810 165
AvaI C'yCGr_G
Cuts at: 0 60 74 100 161 554 975
Size: 60 14 26 61 393 421
Fragments arranged by size:
421 393 61 60 26 14
BanI G'GyrC_C
Cuts at: 0 772 897 975
Size: 772 125 78
Fragments arranged by size:
772 125 78
BanII G_rGCy'C
Cuts at: 0 43 59 869 975
Size: 43 16 810 106
Fragments arranged by size:
810 106 43 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 624 975
Size: 624 351
BbvCI CC'TCA_GC
Cuts at: 0 589 975
Size: 589 386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 366 400 832 866 975
Size: 366 34 432 34 109
Fragments arranged by size:
432 366 109 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 47 975
Size: 47 928
Bme1580I G_kGCm'C
Cuts at: 0 43 75 814 902 975
Size: 43 32 739 88 73
Fragments arranged by size:
739 88 73 43 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 975
Size: 280 695
Bpu10I CC'TnA_GC
Cuts at: 0 434 589 975
Size: 434 155 386
Fragments arranged by size:
434 386 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 975
Size: 853 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 293 694 975
Size: 293 401 281
Fragments arranged by size:
401 293 281
BsaWI w'CCGG_w
Cuts at: 0 329 975
Size: 329 646
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 619 649 975
Size: 619 30 326
Fragments arranged by size:
619 326 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 975
Size: 292 683
BseYI C'CCAG_C
Cuts at: 0 189 274 777 824 975
Size: 189 85 503 47 151
Fragments arranged by size:
503 189 151 85 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 250 975
Size: 108 142 725
Fragments arranged by size:
725 142 108
BsiEI CG_ry'CG
Cuts at: 0 967 975
Size: 967 8
BsiHKAI G_wGCw'C
Cuts at: 0 814 845 975
Size: 814 31 130
Fragments arranged by size:
814 130 31
BsmI GAATG_Cn'
Cuts at: 0 234 975
Size: 234 741
Bsp1286I G_dGCh'C
Cuts at: 0 43 59 75 814 845 869 902 975
Size: 43 16 16 739 31 24 33 73
Fragments arranged by size:
739 73 43 33 31 24 16 16
BspEI T'CCGG_A
Cuts at: 0 329 975
Size: 329 646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 345 975
Size: 345 630
BsrDI GCAATG_nn'
Cuts at: 0 574 722 975
Size: 574 148 253
Fragments arranged by size:
574 253 148
BssHII G'CGCG_C
Cuts at: 0 65 92 975
Size: 65 27 883
Fragments arranged by size:
883 65 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 211 975
Size: 211 764
BstXI CCAn_nnnn'nTGG
Cuts at: 0 258 729 975
Size: 258 471 246
Fragments arranged by size:
471 258 246
Bsu36I CC'TnA_GG
Cuts at: 0 125 975
Size: 125 850
BtgI C'CryG_G
Cuts at: 0 43 224 283 294 728 975
Size: 43 181 59 11 434 247
Fragments arranged by size:
434 247 181 59 43 11
BtsI GCAGTG_nn'
Cuts at: 0 137 676 975
Size: 137 539 299
Fragments arranged by size:
539 299 137
DraIII CAC_nnn'GTG
Cuts at: 0 405 975
Size: 405 570
EaeI y'GGCC_r
Cuts at: 0 221 499 714 964 975
Size: 221 278 215 250 11
Fragments arranged by size:
278 250 221 215 11
EagI C'GGCC_G
Cuts at: 0 964 975
Size: 964 11
EarI CTCTTCn'nnn_
Cuts at: 0 312 937 975
Size: 312 625 38
Fragments arranged by size:
625 312 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 961 975
Size: 280 681 14
Fragments arranged by size:
681 280 14
HaeII r_GCGC'y
Cuts at: 0 190 975
Size: 190 785
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 873 905 975
Size: 873 32 70
Fragments arranged by size:
873 70 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 975
Size: 928 47
MscI TGG'CCA
Cuts at: 0 716 975
Size: 716 259
MslI CAynn'nnrTG
Cuts at: 0 617 670 727 975
Size: 617 53 57 248
Fragments arranged by size:
617 248 57 53
MspA1I CmG'CkG
Cuts at: 0 10 45 975
Size: 10 35 930
Fragments arranged by size:
930 35 10
NcoI C'CATG_G
Cuts at: 0 728 975
Size: 728 247
NspI r_CATG'y
Cuts at: 0 198 975
Size: 198 777
PflMI CCAn_nnn'nTGG
Cuts at: 0 378 405 975
Size: 378 27 570
Fragments arranged by size:
570 378 27
PspOMI G'GGCC_C
Cuts at: 0 39 975
Size: 39 936
PspXI vC'TCGA_Gb
Cuts at: 0 554 975
Size: 554 421
PvuII CAG'CTG
Cuts at: 0 10 975
Size: 10 965
SacII CC_GC'GG
Cuts at: 0 46 975
Size: 46 929
SexAI A'CCwGG_T
Cuts at: 0 401 975
Size: 401 574
SmaI CCC'GGG
Cuts at: 0 62 76 102 975
Size: 62 14 26 873
Fragments arranged by size:
873 62 26 14
SmlI C'TyrA_G
Cuts at: 0 554 868 975
Size: 554 314 107
Fragments arranged by size:
554 314 107
SphI G_CATG'C
Cuts at: 0 198 975
Size: 198 777
SrfI GCCC'GGGC
Cuts at: 0 102 975
Size: 102 873
StyI C'CwwG_G
Cuts at: 0 311 728 800 975
Size: 311 417 72 175
Fragments arranged by size:
417 311 175 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 678 975
Size: 678 297
Tth111I GACn'n_nGTC
Cuts at: 0 628 975
Size: 628 347
XhoI C'TCGA_G
Cuts at: 0 554 975
Size: 554 421
XmaI C'CCGG_G
Cuts at: 0 60 74 100 975
Size: 60 14 26 875
Fragments arranged by size:
875 60 26 14
XmnI GAAnn'nnTTC
Cuts at: 0 511 975
Size: 511 464
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AlfI ApaI ApaLI
AvaI BanI BanII BbsI BbvCI BcgI BglI Bme1580I
BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI
BssHII BstAPI BstXI Bsu36I BtgI BtsI DraIII EaeI
EagI EarI Eco57MI HaeII Hin4I MmeI MscI MslI
MspA1I NcoI NspI PflMI PspOMI PspXI PvuII SacII
SexAI SmaI SmlI SphI SrfI StyI TaqII Tth111I
XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AloI
AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI BsaAI BsaBI BsaHI BsiWI BsmBI BspHI
BsrBI BsrFI BsrGI BssSI BstBI BstEII BstYI BstZ17I
BtgZI ClaI CspCI DraI DrdI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SnaBI
SpeI SspI StuI SwaI TatI TstI XbaI XcmI
ZraI