Restriction map for
pFN21AE4319
(Linear) (Six-base) MAPSORT of: KIEE4319.seq Check: 6475 from: 1 to: 975
KIEE4319.orf
With 182 enzymes: SgfI *
March 22, 2012 17:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 540 975
Size: 540 435
Acc65I G'GTAC_C
Cuts at: 0 58 975
Size: 58 917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 796 975
Size: 199 597 179
Fragments arranged by size:
597 199 179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 223 884 975
Size: 223 661 91
Fragments arranged by size:
661 223 91
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 683 715 975
Size: 683 32 260
Fragments arranged by size:
683 260 32
AlwNI CAG_nnn'CTG
Cuts at: 0 474 975
Size: 474 501
AvaI C'yCGr_G
Cuts at: 0 212 262 975
Size: 212 50 713
Fragments arranged by size:
713 212 50
BanI G'GyrC_C
Cuts at: 0 58 207 975
Size: 58 149 768
Fragments arranged by size:
768 149 58
BanII G_rGCy'C
Cuts at: 0 643 975
Size: 643 332
BbsI GAAGACnn'nnnn_
Cuts at: 0 295 975
Size: 295 680
BglI GCCn_nnn'nGGC
Cuts at: 0 638 975
Size: 638 337
BglII A'GATC_T
Cuts at: 0 740 975
Size: 740 235
BmgBI CAC'GTC
Cuts at: 0 189 975
Size: 189 786
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 331 460 634 706 716 975
Size: 289 42 129 174 72 10 259
Fragments arranged by size:
289 259 174 129 72 42 10
Bpu10I CC'TnA_GC
Cuts at: 0 109 455 748 975
Size: 109 346 293 227
Fragments arranged by size:
346 293 227 109
BsaAI yAC'GTr
Cuts at: 0 607 975
Size: 607 368
BsaBI GATnn'nnATC
Cuts at: 0 778 975
Size: 778 197
BsaWI w'CCGG_w
Cuts at: 0 284 975
Size: 284 691
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 208 238 975
Size: 208 30 737
Fragments arranged by size:
737 208 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 633 975
Size: 232 401 342
Fragments arranged by size:
401 342 232
BseYI C'CCAG_C
Cuts at: 0 474 975
Size: 474 501
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 487 975
Size: 7 480 488
Fragments arranged by size:
488 480 7
BsmI GAATG_Cn'
Cuts at: 0 387 562 975
Size: 387 175 413
Fragments arranged by size:
413 387 175
Bsp1286I G_dGCh'C
Cuts at: 0 643 975
Size: 643 332
BspEI T'CCGG_A
Cuts at: 0 284 975
Size: 284 691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 540 747 975
Size: 540 207 228
Fragments arranged by size:
540 228 207
BsrBI CCG'CTC
Cuts at: 0 357 975
Size: 357 618
BsrDI GCAATG_nn'
Cuts at: 0 159 975
Size: 159 816
BstXI CCAn_nnnn'nTGG
Cuts at: 0 607 975
Size: 607 368
BstYI r'GATC_y
Cuts at: 0 740 975
Size: 740 235
BtsI GCAGTG_nn'
Cuts at: 0 594 975
Size: 594 381
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 115 150 975
Size: 115 35 825
Fragments arranged by size:
825 115 35
EaeI y'GGCC_r
Cuts at: 0 723 975
Size: 723 252
EarI CTCTTCn'nnn_
Cuts at: 0 61 845 916 975
Size: 61 784 71 59
Fragments arranged by size:
784 71 61 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 289 331 460 634 706 716 796
Size: 199 90 42 129 174 72 10 80
Cuts at: 796 975
Size: 179
Fragments arranged by size:
199 179 174 129 90 80 72 42
10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 181 685 975
Size: 181 504 290
Fragments arranged by size:
504 290 181
EcoO109I rG'GnC_Cy
Cuts at: 0 174 975
Size: 174 801
HaeII r_GCGC'y
Cuts at: 0 475 975
Size: 475 500
KpnI G_GTAC'C
Cuts at: 0 62 975
Size: 62 913
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 975
Size: 509 466
MslI CAynn'nnrTG
Cuts at: 0 823 975
Size: 823 152
MspA1I CmG'CkG
Cuts at: 0 94 309 541 765 975
Size: 94 215 232 224 210
Fragments arranged by size:
232 224 215 210 94
PflMI CCAn_nnn'nTGG
Cuts at: 0 242 975
Size: 242 733
PmlI CAC'GTG
Cuts at: 0 607 975
Size: 607 368
PvuII CAG'CTG
Cuts at: 0 94 309 541 975
Size: 94 215 232 434
Fragments arranged by size:
434 232 215 94
StyI C'CwwG_G
Cuts at: 0 931 975
Size: 931 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 427 578 975
Size: 427 151 397
Fragments arranged by size:
427 397 151
Enzymes that do cut:
AarI Acc65I AcuI AlfI AloI AlwNI AvaI BanI
BanII BbsI BglI BglII BmgBI BpmI Bpu10I BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I
BspEI BspMI BsrBI BsrDI BstXI BstYI BtsI CspCI
EaeI EarI Eco57MI EcoNI EcoO109I HaeII KpnI MmeI
MslI MspA1I PflMI PmlI PvuII StyI TaqII
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BlpI Bme1580I BmrI BmtI BplI BpuEI BsaI BsaHI
BsiEI BsiHKAI BsiWI BsmBI BspHI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI