Restriction map for
pFN21AE4318
(Linear) (Six-base) MAPSORT of: KIEE4318.seq Check: 443 from: 1 to: 972
KIEE4318.orf
With 182 enzymes: SgfI *
March 22, 2012 17:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 217 972
Size: 217 755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 972
Size: 103 869
AlwNI CAG_nnn'CTG
Cuts at: 0 453 972
Size: 453 519
ApaI G_GGCC'C
Cuts at: 0 786 841 972
Size: 786 55 131
Fragments arranged by size:
786 131 55
ApaLI G'TGCA_C
Cuts at: 0 352 637 972
Size: 352 285 335
Fragments arranged by size:
352 335 285
AvaI C'yCGr_G
Cuts at: 0 595 972
Size: 595 377
BanII G_rGCy'C
Cuts at: 0 119 786 826 841 856 972
Size: 119 667 40 15 15 116
Fragments arranged by size:
667 119 116 40 15 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 685 719 972
Size: 685 34 253
Fragments arranged by size:
685 253 34
BglI GCCn_nnn'nGGC
Cuts at: 0 505 972
Size: 505 467
BlpI GC'TnA_GC
Cuts at: 0 139 972
Size: 139 833
Bme1580I G_kGCm'C
Cuts at: 0 356 641 786 841 972
Size: 356 285 145 55 131
Fragments arranged by size:
356 285 145 131 55
BmgBI CAC'GTC
Cuts at: 0 570 972
Size: 570 402
BmrI ACTGGGnnnn_n'
Cuts at: 0 854 972
Size: 854 118
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 210 972
Size: 210 762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 205 532 580 840 972
Size: 148 57 327 48 260 132
Fragments arranged by size:
327 260 148 132 57 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 972
Size: 319 653
BsaHI Gr'CG_yC
Cuts at: 0 8 972
Size: 8 964
BsaWI w'CCGG_w
Cuts at: 0 944 972
Size: 944 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 865 894 895 924 972
Size: 865 29 1 29 48
Fragments arranged by size:
865 48 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 159 625 907 972
Size: 159 466 282 65
Fragments arranged by size:
466 282 159 65
BseYI C'CCAG_C
Cuts at: 0 530 736 972
Size: 530 206 236
Fragments arranged by size:
530 236 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 972
Size: 829 143
BsiEI CG_ry'CG
Cuts at: 0 695 972
Size: 695 277
BsiHKAI G_wGCw'C
Cuts at: 0 119 335 356 443 641 748 856 972
Size: 119 216 21 87 198 107 108 116
Fragments arranged by size:
216 198 119 116 108 107 87 21
BsmI GAATG_Cn'
Cuts at: 0 268 655 972
Size: 268 387 317
Fragments arranged by size:
387 317 268
BsmBI CGTCTCn'nnnn_
Cuts at: 0 856 972
Size: 856 116
Bsp1286I G_dGCh'C
Cuts at: 0 119 335 356 443 641 748 786 826
Size: 119 216 21 87 198 107 38 40
Cuts at: 826 841 856 972
Size: 15 15 116
Fragments arranged by size:
216 198 119 116 107 87 40 38
21 15 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 217 320 707 972
Size: 217 103 387 265
Fragments arranged by size:
387 265 217 103
BsrBI CCG'CTC
Cuts at: 0 564 600 972
Size: 564 36 372
Fragments arranged by size:
564 372 36
BsrDI GCAATG_nn'
Cuts at: 0 272 972
Size: 272 700
BssSI C'ACGA_G
Cuts at: 0 634 972
Size: 634 338
BstEII G'GTnAC_C
Cuts at: 0 286 972
Size: 286 686
BstXI CCAn_nnnn'nTGG
Cuts at: 0 651 972
Size: 651 321
Bsu36I CC'TnA_GG
Cuts at: 0 890 972
Size: 890 82
BtgI C'CryG_G
Cuts at: 0 218 500 775 833 972
Size: 218 282 275 58 139
Fragments arranged by size:
282 275 218 139 58
BtsI GCAGTG_nn'
Cuts at: 0 496 972
Size: 496 476
EaeI y'GGCC_r
Cuts at: 0 17 692 972
Size: 17 675 280
Fragments arranged by size:
675 280 17
EagI C'GGCC_G
Cuts at: 0 692 972
Size: 692 280
EarI CTCTTCn'nnn_
Cuts at: 0 46 62 107 972
Size: 46 16 45 865
Fragments arranged by size:
865 46 45 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 333 972
Size: 333 639
EcoICRI GAG'CTC
Cuts at: 0 117 854 972
Size: 117 737 118
Fragments arranged by size:
737 118 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 148 205 532 580 840 972
Size: 103 45 57 327 48 260 132
Fragments arranged by size:
327 260 132 103 57 48 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 721 889 972
Size: 721 168 83
Fragments arranged by size:
721 168 83
EcoO109I rG'GnC_Cy
Cuts at: 0 36 309 507 615 726 782 972
Size: 36 273 198 108 111 56 190
Fragments arranged by size:
273 198 190 111 108 56 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 27 972
Size: 27 945
HaeII r_GCGC'y
Cuts at: 0 559 972
Size: 559 413
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 865 897 972
Size: 865 32 75
Fragments arranged by size:
865 75 32
HincII GTy'rAC
Cuts at: 0 585 972
Size: 585 387
MscI TGG'CCA
Cuts at: 0 19 972
Size: 19 953
MslI CAynn'nnrTG
Cuts at: 0 178 805 972
Size: 178 627 167
Fragments arranged by size:
627 178 167
MspA1I CmG'CkG
Cuts at: 0 432 777 972
Size: 432 345 195
Fragments arranged by size:
432 345 195
NspI r_CATG'y
Cuts at: 0 625 972
Size: 625 347
PpuMI rG'GwC_Cy
Cuts at: 0 36 309 726 972
Size: 36 273 417 246
Fragments arranged by size:
417 273 246 36
PspOMI G'GGCC_C
Cuts at: 0 782 837 972
Size: 782 55 135
Fragments arranged by size:
782 135 55
PstI C_TGCA'G
Cuts at: 0 861 972
Size: 861 111
PvuII CAG'CTG
Cuts at: 0 432 972
Size: 432 540
SacI G_AGCT'C
Cuts at: 0 119 856 972
Size: 119 737 116
Fragments arranged by size:
737 119 116
SacII CC_GC'GG
Cuts at: 0 778 972
Size: 778 194
SapI GCTCTTCn'nnn_
Cuts at: 0 107 972
Size: 107 865
ScaI AGT'ACT
Cuts at: 0 898 972
Size: 898 74
SfcI C'TryA_G
Cuts at: 0 465 857 972
Size: 465 392 115
Fragments arranged by size:
465 392 115
SmlI C'TyrA_G
Cuts at: 0 334 972
Size: 334 638
SphI G_CATG'C
Cuts at: 0 625 972
Size: 625 347
StuI AGG'CCT
Cuts at: 0 817 972
Size: 817 155
TatI w'GTAC_w
Cuts at: 0 896 972
Size: 896 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 894 926 972
Size: 894 32 46
Fragments arranged by size:
894 46 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 972
Size: 180 792
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApaLI AvaI BanII BcgI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BssSI BstEII
BstXI Bsu36I BtgI BtsI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
MscI MslI MspA1I NspI PpuMI PspOMI PstI PvuII
SacI SacII SapI ScaI SfcI SmlI SphI StuI
TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BmtI Bpu10I BsaI BsaAI BsaBI
BsiWI BspEI BspHI BsrFI BsrGI BssHII BstAPI BstBI
BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StyI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI
ZraI