Restriction map for
pFN21AE4317
(Linear) (Six-base) MAPSORT of: KIEE4317.seq Check: 6663 from: 1 to: 969
KIEE4317.orf
With 182 enzymes: SgfI *
March 22, 2012 17:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 744 969
Size: 744 225
AatII G_ACGT'C
Cuts at: 0 367 969
Size: 367 602
AccI GT'mk_AC
Cuts at: 0 191 969
Size: 191 778
AfeI AGC'GCT
Cuts at: 0 547 969
Size: 547 422
AflIII A'CryG_T
Cuts at: 0 103 623 969
Size: 103 520 346
Fragments arranged by size:
520 346 103
AleI CACnn'nnGTG
Cuts at: 0 265 969
Size: 265 704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 969
Size: 283 686
ApaI G_GGCC'C
Cuts at: 0 13 969
Size: 13 956
ApaLI G'TGCA_C
Cuts at: 0 580 969
Size: 580 389
AscI GG'CGCG_CC
Cuts at: 0 346 969
Size: 346 623
AvaI C'yCGr_G
Cuts at: 0 5 177 328 969
Size: 5 172 151 641
Fragments arranged by size:
641 172 151 5
BanI G'GyrC_C
Cuts at: 0 493 969
Size: 493 476
BanII G_rGCy'C
Cuts at: 0 13 178 969
Size: 13 165 791
Fragments arranged by size:
791 165 13
BbeI G_GCGC'C
Cuts at: 0 497 969
Size: 497 472
BbvCI CC'TCA_GC
Cuts at: 0 526 969
Size: 526 443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 171 205 969
Size: 171 34 764
Fragments arranged by size:
764 171 34
BclI T'GATC_A
Cuts at: 0 887 969
Size: 887 82
BglI GCCn_nnn'nGGC
Cuts at: 0 140 149 719 874 969
Size: 140 9 570 155 95
Fragments arranged by size:
570 155 140 95 9
Bme1580I G_kGCm'C
Cuts at: 0 13 584 969
Size: 13 571 385
Fragments arranged by size:
571 385 13
BmgBI CAC'GTC
Cuts at: 0 626 648 969
Size: 626 22 321
Fragments arranged by size:
626 321 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 949 969
Size: 949 20
Bpu10I CC'TnA_GC
Cuts at: 0 421 526 969
Size: 421 105 443
Fragments arranged by size:
443 421 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 969
Size: 211 758
BsaAI yAC'GTr
Cuts at: 0 104 969
Size: 104 865
BsaHI Gr'CG_yC
Cuts at: 0 364 494 518 969
Size: 364 130 24 451
Fragments arranged by size:
451 364 130 24
BsaWI w'CCGG_w
Cuts at: 0 896 969
Size: 896 73
BseYI C'CCAG_C
Cuts at: 0 171 969
Size: 171 798
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 150 541 969
Size: 84 66 391 428
Fragments arranged by size:
428 391 84 66
BsiEI CG_ry'CG
Cuts at: 0 86 153 191 274 578 969
Size: 86 67 38 83 304 391
Fragments arranged by size:
391 304 86 83 67 38
BsiHKAI G_wGCw'C
Cuts at: 0 284 584 969
Size: 284 300 385
Fragments arranged by size:
385 300 284
BsmBI CGTCTCn'nnnn_
Cuts at: 0 632 969
Size: 632 337
Bsp1286I G_dGCh'C
Cuts at: 0 13 178 284 584 969
Size: 13 165 106 300 385
Fragments arranged by size:
385 300 165 106 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 479 744 969
Size: 479 265 225
Fragments arranged by size:
479 265 225
BsrFI r'CCGG_y
Cuts at: 0 73 490 640 823 868 969
Size: 73 417 150 183 45 101
Fragments arranged by size:
417 183 150 101 73 45
BssHII G'CGCG_C
Cuts at: 0 167 232 346 969
Size: 167 65 114 623
Fragments arranged by size:
623 167 114 65
BssSI C'ACGA_G
Cuts at: 0 289 969
Size: 289 680
BstEII G'GTnAC_C
Cuts at: 0 69 969
Size: 69 900
BstXI CCAn_nnnn'nTGG
Cuts at: 0 570 969
Size: 570 399
BtgI C'CryG_G
Cuts at: 0 714 969
Size: 714 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 449 674 816 969
Size: 449 225 142 153
Fragments arranged by size:
449 225 153 142
EaeI y'GGCC_r
Cuts at: 0 75 83 150 271 575 875 969
Size: 75 8 67 121 304 300 94
Fragments arranged by size:
304 300 121 94 75 67 8
EagI C'GGCC_G
Cuts at: 0 83 150 271 575 969
Size: 83 67 121 304 394
Fragments arranged by size:
394 304 121 83 67
EarI CTCTTCn'nnn_
Cuts at: 0 541 969
Size: 541 428
EcoO109I rG'GnC_Cy
Cuts at: 0 9 10 969
Size: 9 1 959
Fragments arranged by size:
959 9 1
EcoRV GAT'ATC
Cuts at: 0 654 969
Size: 654 315
FspI TGC'GCA
Cuts at: 0 448 969
Size: 448 521
FspAI rTGC'GCAy
Cuts at: 0 448 969
Size: 448 521
HaeII r_GCGC'y
Cuts at: 0 245 497 549 816 969
Size: 245 252 52 267 153
Fragments arranged by size:
267 252 245 153 52
HincII GTy'rAC
Cuts at: 0 192 969
Size: 192 777
KasI G'GCGC_C
Cuts at: 0 493 969
Size: 493 476
MslI CAynn'nnrTG
Cuts at: 0 265 844 969
Size: 265 579 125
Fragments arranged by size:
579 265 125
MspA1I CmG'CkG
Cuts at: 0 33 45 155 716 969
Size: 33 12 110 561 253
Fragments arranged by size:
561 253 110 33 12
NaeI GCC'GGC
Cuts at: 0 492 825 870 969
Size: 492 333 45 99
Fragments arranged by size:
492 333 99 45
NarI GG'CG_CC
Cuts at: 0 494 969
Size: 494 475
NgoMIV G'CCGG_C
Cuts at: 0 490 823 868 969
Size: 490 333 45 101
Fragments arranged by size:
490 333 101 45
NotI GC'GGCC_GC
Cuts at: 0 150 969
Size: 150 819
PmlI CAC'GTG
Cuts at: 0 104 969
Size: 104 865
PspOMI G'GGCC_C
Cuts at: 0 9 969
Size: 9 960
PstI C_TGCA'G
Cuts at: 0 755 969
Size: 755 214
SacII CC_GC'GG
Cuts at: 0 717 969
Size: 717 252
SalI G'TCGA_C
Cuts at: 0 190 969
Size: 190 779
SapI GCTCTTCn'nnn_
Cuts at: 0 541 969
Size: 541 428
SbfI CC_TGCA'GG
Cuts at: 0 755 969
Size: 755 214
SfcI C'TryA_G
Cuts at: 0 751 969
Size: 751 218
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 149 719 969
Size: 149 570 250
Fragments arranged by size:
570 250 149
SfoI GGC'GCC
Cuts at: 0 495 969
Size: 495 474
SgrAI Cr'CCGG_yG
Cuts at: 0 490 640 969
Size: 490 150 329
Fragments arranged by size:
490 329 150
SmlI C'TyrA_G
Cuts at: 0 226 969
Size: 226 743
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 466 969
Size: 466 503
TatI w'GTAC_w
Cuts at: 0 914 969
Size: 914 55
ZraI GAC'GTC
Cuts at: 0 365 969
Size: 365 604
Enzymes that do cut:
AarI AatII AccI AfeI AflIII AleI AlfI ApaI
ApaLI AscI AvaI BanI BanII BbeI BbvCI BcgI
BclI BglI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaAI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrFI BssHII BssSI BstEII BstXI BtgI BtgZI
EaeI EagI EarI EcoO109I EcoRV FspI FspAI HaeII
HincII KasI MslI MspA1I NaeI NarI NgoMIV NotI
PmlI PspOMI PstI SacII SalI SapI SbfI SfcI
SfiI SfoI SgrAI SmlI TaqII TatI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AflII AgeI AhdI AloI AlwNI
ApoI AseI AsiSI AvrII BaeI BamHI BbsI BciVI
BfrBI BglII BlpI BmtI BplI BpmI BsaI BsaBI
BsaXI BseRI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrGI BstAPI BstBI BstYI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EciI EcoICRI Eco57MI EcoNI
EcoRI FalI FseI Hin4I HindIII HpaI KpnI MfeI
MluI MmeI MscI NcoI NdeI NheI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SanDI ScaI SexAI SgfI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI