(Linear) (Six-base) MAPSORT of: KIEE4309.seq  Check: 3607  from: 1  to: 954



KIEE4309.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 18:57  ..

AleI CACnn'nnGTG

Cuts at:      0     728     954 
   Size:        728     226
 
ApoI r'AATT_y

Cuts at:      0     399     718     954 
   Size:        399     319     236

  Fragments arranged by size: 

                 399     319     236
 
AvaI C'yCGr_G

Cuts at:      0     516     954 
   Size:        516     438
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     121     954 
   Size:        121     833
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     800     852     954 
   Size:        800      52     102

  Fragments arranged by size: 

                 800     102      52
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     798     954 
   Size:        798     156
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     815     954 
   Size:        815     139
 
BsaBI GATnn'nnATC

Cuts at:      0     146     954 
   Size:        146     808
 
BsiHKAI G_wGCw'C

Cuts at:      0     107     954 
   Size:        107     847
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     157     954 
   Size:        157     797
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     954 
   Size:        107     847
 
BspHI T'CATG_A

Cuts at:      0     306     558     954 
   Size:        306     252     396

  Fragments arranged by size: 

                 396     306     252
 
BsrDI GCAATG_nn'

Cuts at:      0     636     954 
   Size:        636     318
 
BstYI r'GATC_y

Cuts at:      0     775     954 
   Size:        775     179
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     921     954 
   Size:        921      33
 
DraI TTT'AAA

Cuts at:      0     120     647     954 
   Size:        120     527     307

  Fragments arranged by size: 

                 527     307     120
 
EarI CTCTTCn'nnn_

Cuts at:      0     889     954 
   Size:        889      65
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     766     954 
   Size:        766     188
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     798     954 
   Size:        798     156
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     345     377     954 
   Size:        345      32     577

  Fragments arranged by size: 

                 577     345      32
 
MslI CAynn'nnrTG

Cuts at:      0     199     311     349     499     728     954 
   Size:        199     112      38     150     229     226

  Fragments arranged by size: 

                 229     226     199     150     112      38
 
MspA1I CmG'CkG

Cuts at:      0      10     954 
   Size:         10     944
 
NspI r_CATG'y

Cuts at:      0     504     867     954 
   Size:        504     363      87

  Fragments arranged by size: 

                 504     363      87
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     912     954 
   Size:        912      42
 
PsiI TTA'TAA

Cuts at:      0     368     954 
   Size:        368     586
 
PstI C_TGCA'G

Cuts at:      0      27     954 
   Size:         27     927
 
SapI GCTCTTCn'nnn_

Cuts at:      0     889     954 
   Size:        889      65
 
SfcI C'TryA_G

Cuts at:      0      23     954 
   Size:         23     931
 
SphI G_CATG'C

Cuts at:      0     504     954 
   Size:        504     450
 
SwaI ATTT'AAAT

Cuts at:      0     647     954 
   Size:        647     307
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     718     954 
   Size:        718     236
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     832     909     954 
   Size:        832      77      45

  Fragments arranged by size: 

                 832      77      45
 

 Enzymes that do cut:

     AleI     ApoI     AvaI    BciVI     BmrI     BpmI     BsaI    BsaBI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BsrDI    BstYI    CspCI     DraI
     EarI     EciI  Eco57MI    Hin4I     MslI   MspA1I     NspI    PflMI
     PsiI     PstI     SapI     SfcI     SphI     SwaI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     StyI     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI