Restriction map for
pFN21AE4305
(Linear) (Six-base) MAPSORT of: KIEE4305.seq Check: 983 from: 1 to: 915
KIEE4305.orf
With 182 enzymes: SgfI *
March 22, 2012 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 74 769 915
Size: 74 695 146
Fragments arranged by size:
695 146 74
AccI GT'mk_AC
Cuts at: 0 902 915
Size: 902 13
Acc65I G'GTAC_C
Cuts at: 0 117 592 915
Size: 117 475 323
Fragments arranged by size:
475 323 117
AclI AA'CG_TT
Cuts at: 0 645 915
Size: 645 270
AflII C'TTAA_G
Cuts at: 0 150 915
Size: 150 765
AleI CACnn'nnGTG
Cuts at: 0 152 910 915
Size: 152 758 5
Fragments arranged by size:
758 152 5
ApoI r'AATT_y
Cuts at: 0 520 691 915
Size: 520 171 224
Fragments arranged by size:
520 224 171
AvaI C'yCGr_G
Cuts at: 0 443 915
Size: 443 472
BanI G'GyrC_C
Cuts at: 0 117 592 915
Size: 117 475 323
Fragments arranged by size:
475 323 117
BbsI GAAGACnn'nnnn_
Cuts at: 0 139 915
Size: 139 776
BciVI GTATCCnnnnn_n'
Cuts at: 0 691 915
Size: 691 224
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 772 915
Size: 772 143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 590 767 915
Size: 340 250 177 148
Fragments arranged by size:
340 250 177 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 837 915
Size: 42 795 78
Fragments arranged by size:
795 78 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 731 915
Size: 731 184
BseYI C'CCAG_C
Cuts at: 0 259 475 721 915
Size: 259 216 246 194
Fragments arranged by size:
259 246 216 194
BsiHKAI G_wGCw'C
Cuts at: 0 331 915
Size: 331 584
BsmI GAATG_Cn'
Cuts at: 0 309 361 915
Size: 309 52 554
Fragments arranged by size:
554 309 52
BsmBI CGTCTCn'nnnn_
Cuts at: 0 874 915
Size: 874 41
Bsp1286I G_dGCh'C
Cuts at: 0 331 915
Size: 331 584
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 580 769 915
Size: 74 506 189 146
Fragments arranged by size:
506 189 146 74
BsrDI GCAATG_nn'
Cuts at: 0 108 915
Size: 108 807
BstAPI GCAn_nnn'nTGC
Cuts at: 0 335 915
Size: 335 580
BstXI CCAn_nnnn'nTGG
Cuts at: 0 154 193 915
Size: 154 39 722
Fragments arranged by size:
722 154 39
BstYI r'GATC_y
Cuts at: 0 216 915
Size: 216 699
BtgI C'CryG_G
Cuts at: 0 836 848 915
Size: 836 12 67
Fragments arranged by size:
836 67 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 216 915
Size: 216 699
DraI TTT'AAA
Cuts at: 0 780 915
Size: 780 135
EaeI y'GGCC_r
Cuts at: 0 275 851 884 915
Size: 275 576 33 31
Fragments arranged by size:
576 275 33 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 590 767 915
Size: 340 250 177 148
Fragments arranged by size:
340 250 177 148
EcoNI CCTnn'n_nnAGG
Cuts at: 0 70 915
Size: 70 845
EcoO109I rG'GnC_Cy
Cuts at: 0 11 915
Size: 11 904
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 789 821 915
Size: 789 32 94
Fragments arranged by size:
789 94 32
KpnI G_GTAC'C
Cuts at: 0 121 596 915
Size: 121 475 319
Fragments arranged by size:
475 319 121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 768 915
Size: 274 494 147
Fragments arranged by size:
494 274 147
MscI TGG'CCA
Cuts at: 0 277 853 915
Size: 277 576 62
Fragments arranged by size:
576 277 62
MslI CAynn'nnrTG
Cuts at: 0 152 191 910 915
Size: 152 39 719 5
Fragments arranged by size:
719 152 39 5
MspA1I CmG'CkG
Cuts at: 0 838 915
Size: 838 77
NcoI C'CATG_G
Cuts at: 0 848 915
Size: 848 67
PfoI T'CCnGG_A
Cuts at: 0 7 915
Size: 7 908
PpuMI rG'GwC_Cy
Cuts at: 0 11 915
Size: 11 904
PspXI vC'TCGA_Gb
Cuts at: 0 443 915
Size: 443 472
PstI C_TGCA'G
Cuts at: 0 71 465 915
Size: 71 394 450
Fragments arranged by size:
450 394 71
SacII CC_GC'GG
Cuts at: 0 839 915
Size: 839 76
SfcI C'TryA_G
Cuts at: 0 67 461 899 915
Size: 67 394 438 16
Fragments arranged by size:
438 394 67 16
SmlI C'TyrA_G
Cuts at: 0 57 150 443 816 915
Size: 57 93 293 373 99
Fragments arranged by size:
373 293 99 93 57
StuI AGG'CCT
Cuts at: 0 344 915
Size: 344 571
StyI C'CwwG_G
Cuts at: 0 501 848 915
Size: 501 347 67
Fragments arranged by size:
501 347 67
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 691 915
Size: 691 224
XhoI C'TCGA_G
Cuts at: 0 443 915
Size: 443 472
Enzymes that do cut:
AarI AccI Acc65I AclI AflII AleI ApoI AvaI
BanI BbsI BciVI BplI BpmI BpuEI BsaI BseYI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BstAPI BstXI
BstYI BtgI BtgZI DraI EaeI Eco57MI EcoNI EcoO109I
Hin4I KpnI MmeI MscI MslI MspA1I NcoI PfoI
PpuMI PspXI PstI SacII SfcI SmlI StuI StyI
XcmI XhoI
Enzymes that do not cut:
AatII AcuI AfeI AflIII AgeI AhdI AlfI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BbvCI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraIII DrdI
EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI Tth111I XbaI XmaI
XmnI ZraI