(Linear) (Six-base) MAPSORT of: KIEE4303.seq  Check: 7877  from: 1  to: 894



KIEE4303.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:40  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     745     894 
   Size:        275     470     149

  Fragments arranged by size: 

                 470     275     149
 
AlwNI CAG_nnn'CTG

Cuts at:      0     370     447     894 
   Size:        370      77     447

  Fragments arranged by size: 

                 447     370      77
 
ApoI r'AATT_y

Cuts at:      0     522     535     565     894 
   Size:        522      13      30     329

  Fragments arranged by size: 

                 522     329      30      13
 
AvaI C'yCGr_G

Cuts at:      0     170     894 
   Size:        170     724
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     263     894 
   Size:        263     631
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     646     680     894 
   Size:        646      34     214

  Fragments arranged by size: 

                 646     214      34
 
BmgBI CAC'GTC

Cuts at:      0     672     894 
   Size:        672     222
 
BsaBI GATnn'nnATC

Cuts at:      0     213     894 
   Size:        213     681
 
BseYI C'CCAG_C

Cuts at:      0      22     370     894 
   Size:         22     348     524

  Fragments arranged by size: 

                 524     348      22
 
BsmI GAATG_Cn'

Cuts at:      0     454     792     894 
   Size:        454     338     102

  Fragments arranged by size: 

                 454     338     102
 
BsrDI GCAATG_nn'

Cuts at:      0      58     326     503     894 
   Size:         58     268     177     391

  Fragments arranged by size: 

                 391     268     177      58
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     166     894 
   Size:        166     728
 
BstBI TT'CG_AA

Cuts at:      0      66     894 
   Size:         66     828
 
BstYI r'GATC_y

Cuts at:      0     214     894 
   Size:        214     680
 
BtgI C'CryG_G

Cuts at:      0     767     894 
   Size:        767     127
 
EaeI y'GGCC_r

Cuts at:      0     701     894 
   Size:        701     193
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     745     894 
   Size:        275     470     149

  Fragments arranged by size: 

                 470     275     149
 
EcoO109I rG'GnC_Cy

Cuts at:      0     250     894 
   Size:        250     644
 
EcoRV GAT'ATC

Cuts at:      0      90     243     894 
   Size:         90     153     651

  Fragments arranged by size: 

                 651     153      90
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     403     624     894 
   Size:        403     221     270

  Fragments arranged by size: 

                 403     270     221
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     532     564     894 
   Size:        532      32     330

  Fragments arranged by size: 

                 532     330      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     372     894 
   Size:        372     522
 
MscI TGG'CCA

Cuts at:      0     703     894 
   Size:        703     191
 
MspA1I CmG'CkG

Cuts at:      0     370     894 
   Size:        370     524
 
NcoI C'CATG_G

Cuts at:      0     767     894 
   Size:        767     127
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     447     894 
   Size:        447     447
 
PshAI GACnn'nnGTC

Cuts at:      0     105     894 
   Size:        105     789
 
PstI C_TGCA'G

Cuts at:      0     885     894 
   Size:        885       9
 
PvuII CAG'CTG

Cuts at:      0     370     894 
   Size:        370     524
 
SfcI C'TryA_G

Cuts at:      0     881     894 
   Size:        881      13
 
SpeI A'CTAG_T

Cuts at:      0     739     894 
   Size:        739     155
 
StyI C'CwwG_G

Cuts at:      0     767     894 
   Size:        767     127
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AvaI     BbsI     BcgI    BmgBI    BsaBI
    BseYI     BsmI    BsrDI   BstAPI    BstBI    BstYI     BtgI     EaeI
  Eco57MI EcoO109I    EcoRV     FalI    Hin4I     MmeI     MscI   MspA1I
     NcoI    PflMI    PshAI     PstI    PvuII     SfcI     SpeI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI