Restriction map for
pFN21AE4302
(Linear) (Six-base) MAPSORT of: KIEE4302.seq Check: 2792 from: 1 to: 894
KIEE4302.orf
With 182 enzymes: SgfI *
March 22, 2012 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 31 99 894
Size: 31 68 795
Fragments arranged by size:
795 68 31
AflIII A'CryG_T
Cuts at: 0 533 894
Size: 533 361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 152 733 894
Size: 152 581 161
Fragments arranged by size:
581 161 152
ApoI r'AATT_y
Cuts at: 0 457 894
Size: 457 437
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 561 594 894
Size: 561 33 300
Fragments arranged by size:
561 300 33
BamHI G'GATC_C
Cuts at: 0 609 894
Size: 609 285
BanI G'GyrC_C
Cuts at: 0 361 894
Size: 361 533
BglI GCCn_nnn'nGGC
Cuts at: 0 370 894
Size: 370 524
BglII A'GATC_T
Cuts at: 0 290 488 894
Size: 290 198 406
Fragments arranged by size:
406 290 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 315 894
Size: 315 579
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 894
Size: 464 430
BsaWI w'CCGG_w
Cuts at: 0 606 894
Size: 606 288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 562 894
Size: 133 429 332
Fragments arranged by size:
429 332 133
BsmI GAATG_Cn'
Cuts at: 0 605 894
Size: 605 289
BspEI T'CCGG_A
Cuts at: 0 606 894
Size: 606 288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 31 99 894
Size: 31 68 795
Fragments arranged by size:
795 68 31
BsrBI CCG'CTC
Cuts at: 0 90 894
Size: 90 804
BsrGI T'GTAC_A
Cuts at: 0 749 894
Size: 749 145
BssSI C'ACGA_G
Cuts at: 0 785 894
Size: 785 109
BstEII G'GTnAC_C
Cuts at: 0 217 894
Size: 217 677
BstYI r'GATC_y
Cuts at: 0 290 488 609 894
Size: 290 198 121 285
Fragments arranged by size:
290 285 198 121
BtsI GCAGTG_nn'
Cuts at: 0 134 587 659 894
Size: 134 453 72 235
Fragments arranged by size:
453 235 134 72
DrdI GACnn_nn'nnGTC
Cuts at: 0 172 894
Size: 172 722
EaeI y'GGCC_r
Cuts at: 0 50 224 894
Size: 50 174 670
Fragments arranged by size:
670 174 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 789 894
Size: 789 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 894
Size: 464 430
EcoO109I rG'GnC_Cy
Cuts at: 0 513 894
Size: 513 381
EcoRI G'AATT_C
Cuts at: 0 457 894
Size: 457 437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 309 894
Size: 309 585
HaeII r_GCGC'y
Cuts at: 0 82 333 894
Size: 82 251 561
Fragments arranged by size:
561 251 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 669 701 894
Size: 669 32 193
Fragments arranged by size:
669 193 32
HincII GTy'rAC
Cuts at: 0 677 894
Size: 677 217
HindIII A'AGCT_T
Cuts at: 0 803 894
Size: 803 91
MscI TGG'CCA
Cuts at: 0 226 894
Size: 226 668
MslI CAynn'nnrTG
Cuts at: 0 381 715 894
Size: 381 334 179
Fragments arranged by size:
381 334 179
MspA1I CmG'CkG
Cuts at: 0 13 427 638 894
Size: 13 414 211 256
Fragments arranged by size:
414 256 211 13
NspI r_CATG'y
Cuts at: 0 121 894
Size: 121 773
PstI C_TGCA'G
Cuts at: 0 110 141 894
Size: 110 31 753
Fragments arranged by size:
753 110 31
PvuII CAG'CTG
Cuts at: 0 427 638 894
Size: 427 211 256
Fragments arranged by size:
427 256 211
SfcI C'TryA_G
Cuts at: 0 106 137 894
Size: 106 31 757
Fragments arranged by size:
757 106 31
SphI G_CATG'C
Cuts at: 0 121 894
Size: 121 773
StuI AGG'CCT
Cuts at: 0 467 894
Size: 467 427
StyI C'CwwG_G
Cuts at: 0 26 185 522 894
Size: 26 159 337 372
Fragments arranged by size:
372 337 159 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 289 835 894
Size: 265 24 546 59
Fragments arranged by size:
546 265 59 24
TatI w'GTAC_w
Cuts at: 0 749 887 894
Size: 749 138 7
Fragments arranged by size:
749 138 7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 894
Size: 33 861
Enzymes that do cut:
AarI AflIII AlfI ApoI BaeI BamHI BanI BglI
BglII BmrI BpmI BsaWI BsgI BsmI BspEI BspMI
BsrBI BsrGI BssSI BstEII BstYI BtsI DrdI EaeI
EciI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII MscI MslI MspA1I NspI PstI PvuII SfcI
SphI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BsrDI
BsrFI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII EagI EarI EcoICRI
EcoNI EcoRV FseI FspI FspAI HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI