Restriction map for
pFN21AE4288
(Linear) (Six-base) MAPSORT of: KIEE4288.seq Check: 1717 from: 1 to: 5049
KIEE4288.orf
With 182 enzymes: SgfI *
July 3, 2012 18:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 525 2053 2107 2890 3365 3550 4192
Size: 346 179 1528 54 783 475 185 642
Cuts at: 4192 4819 5049
Size: 627 230
Fragments arranged by size:
1528 783 642 627 475 346 230 185
179 54
AfeI AGC'GCT
Cuts at: 0 2398 5049
Size: 2398 2651
AflIII A'CryG_T
Cuts at: 0 1324 1326 1852 5049
Size: 1324 2 526 3197
Fragments arranged by size:
3197 1324 526 2
AhdI GACnn_n'nnGTC
Cuts at: 0 1180 4343 5049
Size: 1180 3163 706
Fragments arranged by size:
3163 1180 706
AlwNI CAG_nnn'CTG
Cuts at: 0 447 1642 5049
Size: 447 1195 3407
Fragments arranged by size:
3407 1195 447
ApaLI G'TGCA_C
Cuts at: 0 4852 5049
Size: 4852 197
ApoI r'AATT_y
Cuts at: 0 393 638 690 1080 1106 1288 1598 1864
Size: 393 245 52 390 26 182 310 266
Cuts at: 1864 3451 3946 4660 5049
Size: 1587 495 714 389
Fragments arranged by size:
1587 714 495 393 390 389 310 266
245 182 52 26
AseI AT'TA_AT
Cuts at: 0 2522 5049
Size: 2522 2527
AvaI C'yCGr_G
Cuts at: 0 1207 2412 3863 5049
Size: 1207 1205 1451 1186
Fragments arranged by size:
1451 1207 1205 1186
AvrII C'CTAG_G
Cuts at: 0 4099 5049
Size: 4099 950
BanI G'GyrC_C
Cuts at: 0 3245 5049
Size: 3245 1804
BanII G_rGCy'C
Cuts at: 0 2419 2868 4811 5049
Size: 2419 449 1943 238
Fragments arranged by size:
2419 1943 449 238
BbsI GAAGACnn'nnnn_
Cuts at: 0 3556 5049
Size: 3556 1493
BbvCI CC'TCA_GC
Cuts at: 0 2666 2923 5049
Size: 2666 257 2126
Fragments arranged by size:
2666 2126 257
BciVI GTATCCnnnnn_n'
Cuts at: 0 2924 3248 5049
Size: 2924 324 1801
Fragments arranged by size:
2924 1801 324
BclI T'GATC_A
Cuts at: 0 1998 2156 5049
Size: 1998 158 2893
Fragments arranged by size:
2893 1998 158
BfrBI ATG'CAT
Cuts at: 0 75 4367 5049
Size: 75 4292 682
Fragments arranged by size:
4292 682 75
BlpI GC'TnA_GC
Cuts at: 0 2875 3087 5049
Size: 2875 212 1962
Fragments arranged by size:
2875 1962 212
Bme1580I G_kGCm'C
Cuts at: 0 990 4856 5049
Size: 990 3866 193
Fragments arranged by size:
3866 990 193
BmrI ACTGGGnnnn_n'
Cuts at: 0 2871 5049
Size: 2871 2178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 2531 2765 3661 5049
Size: 11 2520 234 896 1388
Fragments arranged by size:
2520 1388 896 234 11
Bpu10I CC'TnA_GC
Cuts at: 0 2666 2923 5049
Size: 2666 257 2126
Fragments arranged by size:
2666 2126 257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 3304 4241 5049
Size: 699 2605 937 808
Fragments arranged by size:
2605 937 808 699
BsaI GGTCTCn'nnnn_
Cuts at: 0 906 1486 3874 5049
Size: 906 580 2388 1175
Fragments arranged by size:
2388 1175 906 580
BsaAI yAC'GTr
Cuts at: 0 1327 5049
Size: 1327 3722
BsaBI GATnn'nnATC
Cuts at: 0 1679 5049
Size: 1679 3370
BsaHI Gr'CG_yC
Cuts at: 0 2376 5049
Size: 2376 2673
BsaWI w'CCGG_w
Cuts at: 0 789 5049
Size: 789 4260
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2753 2783 4396 4426 5049
Size: 2753 30 1613 30 623
Fragments arranged by size:
2753 1613 623 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 14 3147 3481 3823 4510 5049
Size: 14 3133 334 342 687 539
Fragments arranged by size:
3133 687 539 342 334 14
BseYI C'CCAG_C
Cuts at: 0 2504 3350 5049
Size: 2504 846 1699
Fragments arranged by size:
2504 1699 846
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 976 3391 4735 5049
Size: 359 617 2415 1344 314
Fragments arranged by size:
2415 1344 617 359 314
BsiHKAI G_wGCw'C
Cuts at: 0 24 3078 3314 3852 4308 4856 5049
Size: 24 3054 236 538 456 548 193
Fragments arranged by size:
3054 548 538 456 236 193 24
BsmI GAATG_Cn'
Cuts at: 0 3095 4369 4928 4977 5049
Size: 3095 1274 559 49 72
Fragments arranged by size:
3095 1274 559 72 49
Bsp1286I G_dGCh'C
Cuts at: 0 24 990 2419 2868 3078 3314 3852 4308
Size: 24 966 1429 449 210 236 538 456
Cuts at: 4308 4811 4856 5049
Size: 503 45 193
Fragments arranged by size:
1429 966 538 503 456 449 236 210
193 45 24
BspEI T'CCGG_A
Cuts at: 0 789 5049
Size: 789 4260
BspHI T'CATG_A
Cuts at: 0 719 4014 5049
Size: 719 3295 1035
Fragments arranged by size:
3295 1035 719
BsrDI GCAATG_nn'
Cuts at: 0 2132 2917 4073 4937 5049
Size: 2132 785 1156 864 112
Fragments arranged by size:
2132 1156 864 785 112
BsrGI T'GTAC_A
Cuts at: 0 3137 4884 5049
Size: 3137 1747 165
Fragments arranged by size:
3137 1747 165
BssSI C'ACGA_G
Cuts at: 0 1483 2018 5049
Size: 1483 535 3031
Fragments arranged by size:
3031 1483 535
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4932 5049
Size: 4932 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 876 3000 5049
Size: 876 2124 2049
Fragments arranged by size:
2124 2049 876
BstYI r'GATC_y
Cuts at: 0 2444 2568 3975 4399 4575 5049
Size: 2444 124 1407 424 176 474
Fragments arranged by size:
2444 1407 474 424 176 124
BtgI C'CryG_G
Cuts at: 0 1503 5049
Size: 1503 3546
BtsI GCAGTG_nn'
Cuts at: 0 2112 5049
Size: 2112 2937
ClaI AT'CG_AT
Cuts at: 0 2526 5049
Size: 2526 2523
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3248 3283 5049
Size: 3248 35 1766
Fragments arranged by size:
3248 1766 35
DraI TTT'AAA
Cuts at: 0 4713 5049
Size: 4713 336
DraIII CAC_nnn'GTG
Cuts at: 0 837 5049
Size: 837 4212
EaeI y'GGCC_r
Cuts at: 0 3276 5049
Size: 3276 1773
EarI CTCTTCn'nnn_
Cuts at: 0 153 205 251 365 577 767 1208 1541
Size: 153 52 46 114 212 190 441 333
Cuts at: 1541 2231 2999 3284 3587 5033 5049
Size: 690 768 285 303 1446 16
Fragments arranged by size:
1446 768 690 441 333 303 285 212
190 153 114 52 46 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4109 5049
Size: 4109 940
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 346 525 2053 2107 2531 2765 2890
Size: 11 335 179 1528 54 424 234 125
Cuts at: 2890 3365 3550 3661 4192 4819 5049
Size: 475 185 111 531 627 230
Fragments arranged by size:
1528 627 531 475 424 335 234 230
185 179 125 111 54 11
EcoO109I rG'GnC_Cy
Cuts at: 0 3646 5049
Size: 3646 1403
EcoRI G'AATT_C
Cuts at: 0 1864 5049
Size: 1864 3185
EcoRV GAT'ATC
Cuts at: 0 2349 5049
Size: 2349 2700
HaeII r_GCGC'y
Cuts at: 0 2400 5049
Size: 2400 2649
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 917 949 1155 1166 1187 1198 1364 1396
Size: 917 32 206 11 21 11 166 32
Cuts at: 1396 2148 2180 2519 2551 3695 3727 3745 3777
Size: 752 32 339 32 1144 32 18 32
Cuts at: 3777 4252 4284 4329 4361 4431 4463 4760 4792
Size: 475 32 45 32 70 32 297 32
Cuts at: 4792 5049
Size: 257
Fragments arranged by size:
1144 917 752 475 339 297 257 206
166 70 45 32 32 32 32 32
32 32 32 32 32 21 18 11
11
HincII GTy'rAC
Cuts at: 0 1768 2141 5049
Size: 1768 373 2908
Fragments arranged by size:
2908 1768 373
HindIII A'AGCT_T
Cuts at: 0 1735 3356 3526 5049
Size: 1735 1621 170 1523
Fragments arranged by size:
1735 1621 1523 170
MfeI C'AATT_G
Cuts at: 0 245 936 5049
Size: 245 691 4113
Fragments arranged by size:
4113 691 245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 749 3717 3800 5049
Size: 615 134 2968 83 1249
Fragments arranged by size:
2968 1249 615 134 83
MslI CAynn'nnrTG
Cuts at: 0 874 2998 3970 4507 5049
Size: 874 2124 972 537 542
Fragments arranged by size:
2124 972 874 542 537
MspA1I CmG'CkG
Cuts at: 0 1345 3303 5049
Size: 1345 1958 1746
Fragments arranged by size:
1958 1746 1345
NcoI C'CATG_G
Cuts at: 0 1503 5049
Size: 1503 3546
NsiI A_TGCA'T
Cuts at: 0 77 4369 5049
Size: 77 4292 680
Fragments arranged by size:
4292 680 77
NspI r_CATG'y
Cuts at: 0 1856 2056 2212 5049
Size: 1856 200 156 2837
Fragments arranged by size:
2837 1856 200 156
PacI TTA_AT'TAA
Cuts at: 0 4901 5049
Size: 4901 148
PasI CC'CwG_GG
Cuts at: 0 3250 5049
Size: 3250 1799
PciI A'CATG_T
Cuts at: 0 1852 5049
Size: 1852 3197
PflMI CCAn_nnn'nTGG
Cuts at: 0 1084 2190 2832 5049
Size: 1084 1106 642 2217
Fragments arranged by size:
2217 1106 1084 642
PfoI T'CCnGG_A
Cuts at: 0 331 1705 5049
Size: 331 1374 3344
Fragments arranged by size:
3344 1374 331
PmlI CAC'GTG
Cuts at: 0 1327 5049
Size: 1327 3722
PpuMI rG'GwC_Cy
Cuts at: 0 3646 5049
Size: 3646 1403
PsiI TTA'TAA
Cuts at: 0 1700 5049
Size: 1700 3349
PstI C_TGCA'G
Cuts at: 0 2496 5049
Size: 2496 2553
PvuII CAG'CTG
Cuts at: 0 1345 3303 5049
Size: 1345 1958 1746
Fragments arranged by size:
1958 1746 1345
SapI GCTCTTCn'nnn_
Cuts at: 0 2999 3587 5033 5049
Size: 2999 588 1446 16
Fragments arranged by size:
2999 1446 588 16
SfcI C'TryA_G
Cuts at: 0 2492 2676 4073 5049
Size: 2492 184 1397 976
Fragments arranged by size:
2492 1397 976 184
SmaI CCC'GGG
Cuts at: 0 3865 5049
Size: 3865 1184
SmlI C'TyrA_G
Cuts at: 0 678 3283 4256 5049
Size: 678 2605 973 793
Fragments arranged by size:
2605 973 793 678
SspI AAT'ATT
Cuts at: 0 300 3803 4021 4734 5049
Size: 300 3503 218 713 315
Fragments arranged by size:
3503 713 315 300 218
StuI AGG'CCT
Cuts at: 0 2938 4987 5049
Size: 2938 2049 62
Fragments arranged by size:
2938 2049 62
StyI C'CwwG_G
Cuts at: 0 1503 1591 3122 3418 4099 5049
Size: 1503 88 1531 296 681 950
Fragments arranged by size:
1531 1503 950 681 296 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2178 2963 3263 5049
Size: 2178 785 300 1786
Fragments arranged by size:
2178 1786 785 300
TatI w'GTAC_w
Cuts at: 0 1849 2319 2550 3137 4884 5049
Size: 1849 470 231 587 1747 165
Fragments arranged by size:
1849 1747 587 470 231 165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1124 1156 1205 1237 4396 4428 5049
Size: 1124 32 49 32 3159 32 621
Fragments arranged by size:
3159 1124 621 49 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1510 2512 5049
Size: 1510 1002 2537
Fragments arranged by size:
2537 1510 1002
XmaI C'CCGG_G
Cuts at: 0 3863 5049
Size: 3863 1186
XmnI GAAnn'nnTTC
Cuts at: 0 706 1307 3966 5049
Size: 706 601 2659 1083
Fragments arranged by size:
2659 1083 706 601
Enzymes that do cut:
AcuI AfeI AflIII AhdI AlwNI ApaLI ApoI AseI
AvaI AvrII BanI BanII BbsI BbvCI BciVI BclI
BfrBI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BsrGI BssSI
BstAPI BstXI BstYI BtgI BtsI ClaI CspCI DraI
DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
HaeII Hin4I HincII HindIII MfeI MmeI MslI MspA1I
NcoI NsiI NspI PacI PasI PciI PflMI PfoI
PmlI PpuMI PsiI PstI PvuII SapI SfcI SmaI
SmlI SspI StuI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AlfI AloI ApaI AscI AsiSI BaeI BamHI BbeI
BcgI BglI BglII BmgBI BmtI BplI BsiEI BsiWI
BsmBI BspMI BsrBI BsrFI BssHII BstBI BstEII BstZ17I
Bsu36I BtgZI DrdI EagI EcoICRI EcoNI FalI FseI
FspI FspAI HpaI KasI KpnI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI PmeI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI
XhoI ZraI